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Glutamatergic cerebellar granule neurons synthesize and secrete reelin in vitro

Published online by Cambridge University Press:  13 August 2009

Mélanie Sinagra ,
Cecilia Gonzalez Campo ,
Danièle Verrier ,
Olivier Moustié ,
Olivier J. Manzoni  and
Pascale Chavis
Mélanie Sinagra
Affiliation:
INSERM U862, Neurocentre Magendie, Pathophysiology of Synaptic Plasticity, 146 rue Léo Saignat, Bordeaux, F-33000, France Université de Bordeaux, Bordeaux, F-33000, France
Cecilia Gonzalez Campo
Affiliation:
INSERM U862, Neurocentre Magendie, Pathophysiology of Synaptic Plasticity, 146 rue Léo Saignat, Bordeaux, F-33000, France Université de Bordeaux, Bordeaux, F-33000, France
Danièle Verrier
Affiliation:
INSERM U862, Neurocentre Magendie, Pathophysiology of Synaptic Plasticity, 146 rue Léo Saignat, Bordeaux, F-33000, France Université de Bordeaux, Bordeaux, F-33000, France
Olivier Moustié
Affiliation:
INSERM U862, Neurocentre Magendie, Pathophysiology of Synaptic Plasticity, 146 rue Léo Saignat, Bordeaux, F-33000, France
Olivier J. Manzoni
Affiliation:
INSERM U862, Neurocentre Magendie, Pathophysiology of Synaptic Plasticity, 146 rue Léo Saignat, Bordeaux, F-33000, France Université de Bordeaux, Bordeaux, F-33000, France
Pascale Chavis*
Affiliation:
INSERM U862, Neurocentre Magendie, Pathophysiology of Synaptic Plasticity, 146 rue Léo Saignat, Bordeaux, F-33000, France Université de Bordeaux, Bordeaux, F-33000, France
*
Correspondence should be addressed to: Pascale Chavis, INSERM U862, Neurocentre Magendie, Pathophysiology of Synaptic Plasticity, 146 rue Léo Saignat, Bordeaux, F-33000, France phone: (+33) 5 57573771 email: pascale.chavis@inserm.fr
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Abstract

In the postnatal forebrain, the extracellular matrix protein reelin is expressed and secreted by subsets of GABAergic neurons, whereas in the cerebellum reelin is detected in glutamatergic cells of the granule cell layer. Thus, various regions of the postnatal brain present different patterns of reelin expression, whose significance remains unknown. We combined immunocytochemical and pharmacological approaches to characterize the phenotypic and temporal profiles of reelin expression in dissociated cultures of cerebellar granule neurons. A single type of reelin immunoreactivity, identified by a punctate labelling, was present in the somata of the majority of neurons. This immunoreactivity was observed throughout maturation and was exclusively present in glutamatergic neurons expressing the vesicular glutamate transporter 1. Neurons containing the reelin receptors apolipoprotein E receptor 2 (Apoer2) and very low-density lipoprotein receptor (Vldlr) represented about 80% of cerebellar neurons. The vast majority of reelin-positive neurons coexpressed Apoer2, suggesting that reelin immunoreactivity resulted in part from receptor-bound reelin. Inhibition of protein synthesis with cycloheximide completely abolished reelin immunoreactivity. In contrast, blocking protein secretion with brefeldin A did not affect the proportion of punctate neurons but revealed a subpopulation of neurons characterized by a solid reelin staining. These data show for the first time that a homogeneous population of glutamatergic neurons can synthesize and secrete reelin in cerebellar granule cells in vitro.

Keywords

Reelinextracellular matrixglutamatergic neuronssecretioncerebellar granule cells
Type
Research Article
Information
Neuron Glia Biology , Volume 4 , Special Issue 3: Cell Adhesion and Extracellular Matrix Molecules in Synaptic Plasticity , August 2008 , pp. 189 - 196
Copyright
Copyright © Cambridge University Press 2009

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