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Histochemical demonstration of acetylcholinesterase in sporocysts of Schistosoma mansoni (Trematoda)

Published online by Cambridge University Press:  06 April 2009

Joseph J. Diconza  and
Paul F. Basch
Joseph J. Diconza
Affiliation:
Department of Family, Community and Preventive Medicine, Stanford University, Stanford, California94305
Paul F. Basch
Affiliation:
Department of Family, Community and Preventive Medicine, Stanford University, Stanford, California94305
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Extract

The distribution of acetylcholinesterase in mother and daughter sporocysts of Schistosoma mansoni was studied histochemically. In young mother sporocysts derived from miracidia cultured in vitro the miracidial neural mass and flame cells were shown to persist. The nerve trunks and commissures, as well as papillae, are apparently lost in the transformation process. In young daughter sporocysts freshly dissected from mother sporocysts there was little enzyme activity except for a sparse distribution in the tegument. After cultivation, intense enzyme activity was associated with developing cercarial embryos. A similar distribution of activity was observed in older daughter sporocysts obtained from the digestive gland of snails. No evidence of flame cells, neural mass, or commissures was detected in daughter sporocysts by the methods employed.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 2 , October 1975 , pp. 305 - 310
Copyright
Copyright © Cambridge University Press 1975

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References

Basch, P. F. & DiConza, J. J. (1973). Primary cultures of embryonic cells from the snail Biomphalaria glabrata. The American Journal of Tropical Medicine and Hygiene 22, 805–13.CrossRefGoogle ScholarPubMed
Basch, P. F. & DiConza, J. J. (1974). The miracidium-sporocyst transition in Schistosoma mansoni: Surface changes in vitro with ultrastructural correlation. The Journal of Parasitology 60, 935–41.CrossRefGoogle ScholarPubMed
Bruckner, D. A. & Voge, M. (1974). The nervous system of larval Schistosoma mansoni as revealed by acetylcholinesterase staining. The Journal of Parasitology 60, 437–46.CrossRefGoogle ScholarPubMed
Bueding, E., Schiller, E. L. & Bourgeois, J. G. (1967). Some physiological, biochemical, and morphologic effects of tris (p-aminophenyl) carbonium salts (TAC) on Schistosoma mansoni. The American Journal of Tropical Medicine and Hygiene 16, 500–15.CrossRefGoogle ScholarPubMed
Cheng, T. C. & Bier, J. W. (1972). Studies on molluscan schistosomiasis: An analysis of the development of the cercaria of Schistosoma mansoni. Parasitology 64, 129–41.CrossRefGoogle ScholarPubMed
DiConza, J. J. & Basch, P. F. (1974). Axenic cultivation of Schistosoma mansoni daughter sporocysts. The Journal of Parasitology 60, 757–63.CrossRefGoogle ScholarPubMed
Friedenberg, R. M. & Seligman, A. M. (1972). Acetylcholinesterase at the myoneural junction: cytochemical ultrastructure and some biochemical considerations. Journal of Histochemistry and Cytochemistry 20, 771–92.CrossRefGoogle ScholarPubMed
Fripp, P. J. (1967). Histochemical localization of esterase activity in schistosomes. Experimental Parasitology 21, 380–90.CrossRefGoogle ScholarPubMed
Krvavica, S., Lui, A. & Bečejac, S. (1967). Acetylcholinesterase and butyrylcholinesterase in the liver fluke (Fasciola hepatica). Experimental Parasitology 21, 240–8.CrossRefGoogle Scholar
Lewert, R. M. & Hopkins, D. R. (1965). Cholinesterase activity in Schistosoma mansoni cercariae. The Journal of Parasitology 51, 616.CrossRefGoogle Scholar
Pearse, A. G. E. (1960). Histochemistry Theoretical and Applied, 2nd edn.Boston: Little, Brown and Company.Google Scholar
Pearson, B. & Grose, F. (1959). Further histochemical studies of esterases by 5-bromoindoxylacetate. Archives of Pathology 67, 324–32.Google Scholar
Pepler, W. J. (1958). Histochemical demonstration of an acetylcholinesterase in the ova of Schistosoma mansoni. The Journal of Histochemistry and Cytochemistry 6, 139–41.CrossRefGoogle ScholarPubMed
Rybicka, K. (1967). Embryogenesis in Hymenolepis diminuta V. Acetylcholinesterase in embryos. Experimental Parasitology 20, 263–6.CrossRefGoogle ScholarPubMed
Schwabe, C. W., Koussa, M. & Acra, A. N. (1961). Host-parasite relationships in Echinococcosis IV. Acetylcholinesterase and permeability regulation in the hydatid cyst wall. Comparative Biochemistry and Physiology 2, 161–72.CrossRefGoogle ScholarPubMed