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Molecular characterization of Theileria parasites: application to the epidemiology of theileriosis in Zimbabwe

Published online by Cambridge University Press:  06 April 2009

R. P. Bishop ,
P.R. Spooner ,
G. K. Kanhai ,
J. Kiarie ,
A. A. Latif ,
T. Hove ,
S. Masaka  and
T. T. Dolan
R. P. Bishop
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709 Nairobi, Kenya
P.R. Spooner
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709 Nairobi, Kenya
G. K. Kanhai
Affiliation:
FAO Project, GCP/ZIM/013/DEN, P.O. Box 3730, Harare, Zimbabwe
J. Kiarie
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709 Nairobi, Kenya
A. A. Latif
Affiliation:
FAO Project, GCP/ZIM/013/DEN, P.O. Box 3730, Harare, Zimbabwe
T. Hove
Affiliation:
Veterinary Research Laboratory, P.O. Box 8101, Causeway, Harare, Zimbabwe
S. Masaka
Affiliation:
Veterinary Research Laboratory, P.O. Box 8101, Causeway, Harare, Zimbabwe
T. T. Dolan
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709 Nairobi, Kenya
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Extract

Forty Theileria schizont-infected lymphocyte culture isolates from Zimbabwe were characterized using a panel of antischizont monoclonal antibodies (MAbs) and 4 Theileria parva DNA probes containing cloned extrachromosomal element, Tpr repetitive, ribosomal and telomeric sequences. The Theileria isolates were assigned as T. parva or T. taurotragi on the basis of reactivities with MAbs and restriction fragment length polymorphisms (RFLPs) detected using the extra chromosomal element probe. Cattle-derived T. parva isolates were relatively homogeneous on the basis of reactivities with MAbs and RFLPs detected using Tpr repetitive and ribosomal DNA probes. In contrast to previous results from Kenya, most of the cattle-derived isolates from Zimbabwe exhibited very similar Tpr restriction fragment patterns, although the Tpr genotypes of buffalo-derived isolates were heterogeneous. This suggests that selection for a particular Tpr genotype may be occurring in cattle. Many isolates with similar Tpr genotypes were differentiated by RFLPs detected using the telomeric DNA probe. The T. parva Boleni immunizing stock was distinguished from all other isolates by telomeric RFLPs. The T. parva Boleni Tpr repetitive DNA probe cross-hybridized with T. taurotragi DNA and detected RFLPs between different T. taurotragi isolates.

Keywords

Theileria parvaTheileria taurotragiDNA probemonoclonal antibodySouthern blotRFLPs
Type
Research Article
Information
Parasitology , Volume 109 , Issue 5 , December 1994 , pp. 573 - 581
Copyright
Copyright © Cambridge University Press 1994

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