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Specific systemic IgG1, IgG2 and IgM responses in pigs immunized with infective eggs or selected antigens of Ascaris suum

Published online by Cambridge University Press:  09 October 2003

E. FRONTERA ,
A. CARRÓN ,
F. J. SERRANO ,
A. ROEPSTORFF ,
D. REINA  and
I. NAVARRETE
E. FRONTERA
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
A. CARRÓN
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
F. J. SERRANO
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
A. ROEPSTORFF
Affiliation:
Danish Center for Experimental Parasitology, The Royal Veterinary and Agricultural University, Dyerlaegevej 100, DK-1870 Frederiksberg C, Copenhagen, Denmark
D. REINA
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
I. NAVARRETE
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
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Abstract

A total of 35 pigs aged 15 weeks old, and 21 pigs aged 8 weeks old were divided into 7 groups. Groups 1 and 2 were uninfected and challenge control groups, respectively. Groups 3 and 4 were infected weekly with 6 increasing doses of Ascaris suum eggs, and group 4 was additionally treated with pyrantel. Groups 5, 6, and 7 were immunized weekly with the 14, 42, or 97 kDa fractions from adult worms, respectively. Animals of groups 2–7 were challenged with 10 000 A. suum eggs 7 days after the last infection/immunization. Serum was sampled weekly and specific IgG1, IgG2, and IgM responses were measured. Pigs of groups 5, 6, and 7 showed high IgG1 and IgG2 responses especially against adult worms antigens, while infected groups had high IgG1 and IgM responses, especially against larva. The IgG1 responses were negatively correlated to the numbers of larvae in the lungs, and positively associated with the liver white spot numbers. There was a positive correlation between IgG2 and the numbers of white spots and lung larvae, while IgM was negatively correlated with these parasitological measures. These findings are discussed and it is suggested that acquired resistance against A. suum larvae is correlated with the induction of IgG1 and IgM, and not with IgG2, and that future vaccination protocols may focus on inducing the Th2 activity.

Keywords

Ascaris suumantigensIgG1IgG2IgMpigs
Type
Research Article
Information
Parasitology , Volume 127 , Issue 3 , September 2003 , pp. 291 - 298
Copyright
© 2003 Cambridge University Press

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