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Trypanosoma (Nannomonas) godfreyi sp. nov. from tsetse flies in The Gambia: biological and biochemical characterization

Published online by Cambridge University Press:  06 April 2009

J. J. McNamara ,
G. Mohammed  and
W. C. Gibson
J. J. McNamara
Affiliation:
MRC Trypanosomiasis Research Group, University of Bristol, Churchill Building, Langford, Bristol BS18 7DY, UK
G. Mohammed
Affiliation:
Department of Veterinary Surgery and Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Samaru, Zaria, Nigeria Department of Pathology and Microbiology, University of Bristol, Churchill Building, Langford, Bristol BS18 7DY, UK
W. C. Gibson
Affiliation:
Department of Pathology and Microbiology, University of Bristol, Churchill Building, Langford, Bristol BS18 7DY, UK
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Summary

We provide evidence from isoenzyme analysis, hybridization with repetitive DNA probes, behavioural studies and morphometrics that 4 trypanosome isolates from Glossina morsitans submorsitans in The Gambia constitute a new species now named Trypanosoma (Nannomonas) godfreyi. The bloodstream trypomastigotes of T. (N.) godfreyi are relatively small with a mean length of 13·7 μm (range: 9·1–21·8 μm) and a mean width of 1·65 μm (range: 0·65–2·69 μm). There is no free flagellum and the marginal kinetoplast is subterminal to a rounded posterior end; the undulating membrane is usually conspicuous. As with other Nannomonas, T. godfreyi developed in the midgut and proboscis of Glossina and infections matured in 21–28 days in laboratory G. m. morsitans. In The Gambia the normal vertebrate host appears to be the warthog, Phacochoerus aethiopicus, although elsewhere other wild and domestic suids may also be implicated in the life-cycle. T. godfreyi was identified unequivocally using a 380 bp DNA probe specific for a major genomic repeat sequence; its isoenzyme profile distinguished it clearly from T. simiae and three strain groups of T. congolense: savannah, riverine-forest and kilifi.

Keywords

trypanosomeNannomonasTrypanosoma godfreyiporcine trypanosomiasis
Type
Research Article
Information
Parasitology , Volume 109 , Issue 4 , November 1994 , pp. 497 - 509
Copyright
Copyright © Cambridge University Press 1994

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