Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-10T11:03:32.107Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector

Published online by Cambridge University Press:  01 January 1999

R. J. DILLON  and
R. P. LANE
R. J. DILLON
Affiliation:
Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY
R. P. LANE
Affiliation:
The Wellcome Trust, 183 Euston Rd, London NW1 2BE
Get access Rights & Permissions [Opens in a new window]

Abstract

Binding to the midgut microvillar surface in the sandfly Phlebotomus papatasi is a prerequisite for successful development of Leishmania major within the gut of the vector. This paper describes a method for detecting microvillar-associated proteins which act as ligands for the parasite surface glycoconjugate lipophosphoglycan (LPG). Adhesion of LPG to midgut proteins was visualized by probing midgut extracts with LPG using a Western ligand blotting technique. Procyclic L. major LPG bound to a microvillar polypeptide band of 65 kDa (estimated in the non-reduced state) and bound variably to several lower molecular weight bands, probably degradation products or subunits of the primary binding polypeptides. Specificity of binding was confirmed by co-incubating biotinylated LPG with an LPG-specific mAb which resulted in a great reduction in binding.

Keywords

Leishmania majorlipophosphoglycanPhlebotomus papatasimicrovillimidgut
Type
Research Article
Information
Parasitology , Volume 118 , Issue 1 , January 1999 , pp. 27 - 32
Copyright
1999 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)