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Diet-induced obesity promotes systemic inflammation and increased susceptibility to murine visceral leishmaniasis

Published online by Cambridge University Press:  21 July 2016

GLÊNIA DAROS SARNÁGLIA ,
LUCIANA POLACO COVRE ,
FAUSTO EDMUNDO LIMA PEREIRA ,
HERBERT LEONEL DE MATOS GUEDES ,
ANA MARIA CAETANO FARIA ,
REYNALDO DIETZE ,
RODRIGO RIBEIRO RODRIGUES ,
TATIANI UCELI MAIOLI  and
DANIEL CLÁUDIO OLIVIERA GOMES
GLÊNIA DAROS SARNÁGLIA
Affiliation:
Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo – UFES, Vitória, Brazil
LUCIANA POLACO COVRE
Affiliation:
Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo – UFES, Vitória, Brazil
FAUSTO EDMUNDO LIMA PEREIRA
Affiliation:
Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo – UFES, Vitória, Brazil
HERBERT LEONEL DE MATOS GUEDES
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
ANA MARIA CAETANO FARIA
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
REYNALDO DIETZE
Affiliation:
Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo – UFES, Vitória, Brazil
RODRIGO RIBEIRO RODRIGUES
Affiliation:
Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo – UFES, Vitória, Brazil
TATIANI UCELI MAIOLI
Affiliation:
Departamento de Nutrição, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
DANIEL CLÁUDIO OLIVIERA GOMES*
Affiliation:
Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo – UFES, Vitória, Brazil Núcleo de Biotecnologia, Universidade Federal do Espírito Santo – UFES, Vitória, Brazil
*
*Corresponding author. Laboratório de Imunobiologia, Núcleo de Doenças Infecciosas/Núcleo de Biotecnologia, Universidade Federal do Espirito Santo – UFES, Av. Marechal Campos, 1468 – Maruípe, Vitória – ES, Cep 29040-091, Brazil. E-mail: dgomes@ndi.ufes.br
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Summary

Obesity is the main causal factor for metabolic syndrome and chronic systemic inflammation, which impacts on immune function and increases susceptibility to pathogens. Here, we investigated the effect of obesity on the outcome of visceral leishmaniasis caused by Leishmaniasis infantum chagasi. C57BL/6 mice fed with high-sugar and butter diet (HSB) showed a significant increase in body weight, adiposity index and morphological changes in adipocyte. To investigate the consequences of obesity on the specific immunity against Leishmania, both control and HSB diet groups were infected with 107L. infantum chagasi promastigotes in the eighth-week after diet started and euthanized 4 weeks later. HSB-diet fed mice exhibited a significantly higher parasite burden in both liver and spleen compared with control- diet group. Gonadal adipocyte tissue from HSB-diet mice showed increased TNF-α, IL-6 and leptin and diminished IL-10 production compared with control. Cytokines production analysis in the spleen and liver from these animals also demonstrated higher production of IFN-γ, TNF-α, IL-6 and nitric oxide and diminished production of IL-10 and TGF-β, which correlate with inflammatory foci and the cell hyperplasia observed. Taken together, obesity can interfere with responses to pathogen-derived signals and impair the development of protective anti-Leishmania immunity.

Keywords

Leishmania infantum chagasihigh-sugar and butter dietexperimental obesity
Type
Research Article
Information
Parasitology , Volume 143 , Issue 12 , October 2016 , pp. 1647 - 1655
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Copyright
Copyright © Cambridge University Press 2016 

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