Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-10T11:38:39.044Z Has data issue: false hasContentIssue false
  • English
  • Français

Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences

Published online by Cambridge University Press:  06 April 2009

B. A. Allsopp ,
H. A. Baylis ,
M. T. E. P. Allsoppi ,
T. Cavalier-Smith ,
R. P. Bishop ,
D. M. Carrington ,
B. Sohanpal  and
P. Spooner
B. A. Allsopp*
Affiliation:
University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1QW, UK
H. A. Baylis*
Affiliation:
University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1QW, UK
M. T. E. P. Allsoppi*
Affiliation:
Kings College London, Department of Biophysics, 26–29 Drury Lane, London WC2B 5RL, UK
T. Cavalier-Smith*
Affiliation:
Kings College London, Department of Biophysics, 26–29 Drury Lane, London WC2B 5RL, UK
R. P. Bishop
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
D. M. Carrington
Affiliation:
University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1QW, UK
B. Sohanpal
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
P. Spooner
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
*
*Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa (address for reprint requests and correspondence).
†University of York, Department of Biology, Heslington, York YO1 5DD, UK.
*Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa (address for reprint requests and correspondence).
‡University of British Columbia, Department of Botany, 6270 University Boulevard, Vancouver V6T 1Z4, Canada.
Get access Rights & Permissions [Opens in a new window]

Summary

The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central (variable) region of the srRNA genes of T. annulata, T. taurotragi, T. mutans and two unidentified parasites referred to as Theileria sp. (buffalo) and Theileria sp. (Marula) were obtained. An alignment of the sequences was generated from which 6 oligonucleotide probes, corresponding to species-specific regions, were designed. These probes were demonstrated to provide unequivocal identification of each of the 6 species either by direct detection of parasite srRNA or by hybridization to amplified parasite srRNA genes. The probes were not able to distinguish buffalo-derived T. parva, the causal agent of Corridor disease, from cattle-derived T. parva, the causal agent of East Coast fever.

Keywords

small ribosomal RNA genesgene amplification (PCR)sequence homologyoligonucleotide probesSporozoa genetics.
Type
Research Article
Information
Parasitology , Volume 107 , Issue 2 , August 1993 , pp. 157 - 165
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Allsopp, B. A. & Allsopp, M. T. E. P. (1988). Theileria parva: genomic DNA studies reveal intra-specific sequence diversity. Molecular and Biochemical Parasitology 28, 77–84.CrossRefGoogle ScholarPubMed
Allsopp, B. A., Carrington, D. M., Baylis, H. A., Sohal, S. K., Dolan, T. T. & Iams, K. (1989). Improved characterization of Theileria parva isolates using the polymerase chain reaction and oligonucleotide probes. Molecular and Biochemical Parasitology 35, 137–48.CrossRefGoogle ScholarPubMed
Anon. (1989). In Theileriosis in Eastern, Central and Southern Africa (ed. Dolan, T. T.), pp. 182–6. Nairobi: ILRAD.Google Scholar
Baylis, H. A., Sohal, S. K., Carrington, D. M., Bishop, R. P. & Allsopp, B. A. (1991). An unusual repetitive gene family in Theileria parva which is stage- specifically transcribed. Molecular and Biochemical Parasitology 49, 133–42.CrossRefGoogle ScholarPubMed
Bishop, R. P., Sohanpal, B., Kariuki, D. P., Young, A. S., Nene, Va., Baylis, H. A., Allsopp, B. A., Spooner, P. R., Dolan, T. T. & Morzaria, S. P. (1992). Detection of a carrier state in Theileria parva-infected cattle using the polymerase chain reaction. Parasitology 104, 215–32.CrossRefGoogle ScholarPubMed
Brocklesby, D. W. & Hawking, F. (1958). Growth of Theileria annulata and T. parva in tissue cultures. Transactions of the Royal Society of Tropical Medicine and Hygiene 52, 414–20.CrossRefGoogle Scholar
Brocklesby, D. W., Barnett, S. F. & Scott, G. R. (1961). Morbidity and mortality rates in East Coast fever (Theileria parva infection) and their application to drug screening procedures. British Veterinary Journal 117, 529–31.CrossRefGoogle Scholar
Church, G. M. & Gilbert, W. (1984). Genomic sequencing. Proceedings of the National Academy of Sciences, USA 81, 1991–5.CrossRefGoogle ScholarPubMed
Conrad, P. A., Iams, K., Brown, W. C., Sohanpal, B. & Ole-Moi Yoi, O. (1987 a). DNA probes detect genomic diversity in Theileria parva stocks. Molecular and Biochemical Parasitology 25, 213–26.CrossRefGoogle ScholarPubMed
Conrad, P. A., Stagg, D. A., Grootenhuis, J. G., Irvin, A. D., Newson, J., Njamunggeh, R. E. G., Rossiter, P. B. & Young, A. S. (1987 b). Isolation of Theileria parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies. Parasitology 94, 413–23.CrossRefGoogle ScholarPubMed
Conrad, P. A., Ole-Moi Yoi, O. K., Baldwin, C. L., Dolan, T. T., O'callaghan, C. J., Njamunggeh, R. E. G., Grootenhuis, J. G., Stagg, D. A., Leitch, B. L. & Young, A. S. (1989). Characterization of buffalo- derived theilerial parasites with monoclonal antibodies and DNA probes. Parasitology 98, 179–88.CrossRefGoogle ScholarPubMed
De Vos, A. J. (1982). The identity of bovine Theileria spp. in South Africa. M. Vet. Med. thesis, University of Pretoria.Google Scholar
Elwood, H. J., Olsen, G. J. & Sogin, M. L. (1985). The small subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata. Molecular Biology and Evolution 5, 399–410.Google Scholar
Gajadhar, A. A., Marquardt, W. C., Hall, R., Gunderson, J., Arizita-Carmona, E. V. & Sogin, M. L. (1991). Ribosomal RNA sequences of Sarcocystis muris, Crypthecodinium cohnii and Theileria annulata reveal evolutionary relationships among apicomplexans, dinofiagellates and ciliates. Molecular and Biochemical Parasitology 45, 147–54.CrossRefGoogle Scholar
Grootenhuis, J. G., Young, A. S., Dolan, T. T. & Stagg, D. A. (1979). Characteristics of Theileria species (eland) in eland and cattle. Research in Veterinary Science 27, 59–68.CrossRefGoogle ScholarPubMed
Higgins, D. G. & Sharp, P. M. (1989). Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5, 151–3.Google ScholarPubMed
Irvin, A. D. (1987). Characterization of species and strains of Theileria. Advances in Parasitology 26, 145–79.CrossRefGoogle ScholarPubMed
Irvin, A. D., Purnell, R. E., Brown, C. G. D., Cunningham, M. P., Ledger, M. A. & Payne, R. (1974). The applications of an indirect method of infecting ticks with piroplasms for use in the isolation of field infections. British Veterinary Journal 130, 280–7.CrossRefGoogle ScholarPubMed
Jura, W. G., Brown, C. G. D. & Kelly, B. (1983). Fine structure and invasive behaviour of the early developmental stages of Theileria annulata in vitro. Veterinary Parasitology 12, 31–44.CrossRefGoogle ScholarPubMed
Koch, H. T., Ocama, J. G., Munatswa, F. C., Byrom, B., Norval, R. A., Spooner, P. R., Conrad, P. A. & Irvin, A. D. (1988). Isolation and characterization of bovine Theileria parasites in Zimbabwe. Veterinary Parasitology 28, 19–32.CrossRefGoogle ScholarPubMed
Kreitmann, M. & Landweber, L. F. (1989). A strategy for producing single-stranded DNA in the polymerase chain reaction: a direct method for genomic sequencing. Gene Analysis Techniques 6, 84–8.CrossRefGoogle Scholar
Morzaria, S. P., Spooner, S. P., Bishop, R. P., Musoke, A. J. & Young, A. S. (1990). SfiI and NotI polymorphisms in Theileria stocks detected by pulsed field gel electrophoresis. Molecular and Biochemical Parasitology 40, 203–12.CrossRefGoogle ScholarPubMed
Neitz, W. O. (1948). Studies on East Coast fever. South African Science 1, 133–5.Google Scholar
Neitz, W. O. (1955). Corridor disease: a fatal form of bovine theileriosis encountered in Zululand. Bulletin of Epizootic Diseases of Africa 3, 121–3.Google Scholar
Potgieter, F. T., Stoltsz, W. H., Blouin, E. F. & Roos, J. A. (1988). Corridor disease in South Africa: a review of the current status. Journal of the South African Veterinary Association 59, 155–60.Google ScholarPubMed
Purnell, R. E. (1977). East Coast fever: some recent research in East Africa. Advances in Parasitology 15, 82–132.Google ScholarPubMed
Saiki, R. K., Bugawan, T. L., Horn, G. T., Mullis, K. B. & Erlich, H. A. (1986). Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele- specific oligonucleotide probes. Nature, London 324, 163–6.CrossRefGoogle Scholar
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. & Erlich, H. A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–91.CrossRefGoogle ScholarPubMed
Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74, 5463–7.CrossRefGoogle ScholarPubMed
Schreuder, B. E., Uilenberg, G. & Tondeur, W. (1977). Studies on Theileriidae (Sporozoa) in Tanzania. VIII. Experiments with African buffalo (Syncerus caffer). Zeitschrift für Tropenmedizin und Parasitologie 28, 367–71.Google ScholarPubMed
Sogin, M. L. (1990). Amplification of ribosomal RNA genes for molecular evolution studies. In PCR Protocols (ed. Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. H.), pp. 307–322. San Diego: Academic Press.Google Scholar
Stagg, D. A., Young, A. S., Leitch, B. L., Grootenhuis, J. G. & Dolan, T. T. (1983). Infection of mammalian cells with Theileria species. Parasitology 86, 243–54.CrossRefGoogle ScholarPubMed
Uilenberg, G. (1981). Theilerial species of domestic livestock. In Advances in the Control of Theileriosis (ed. Irvin, A. D., Cunningham, M. P. & Young, A. S.), pp. 4–37. The Hague: Martinus Nijhoff.CrossRefGoogle Scholar
Waters, A. P. & McCutchan, T. F. (1989). Rapid, sensitive diagnosis of malaria based on ribosomal RNA. Lancet 1 (8651), 1343–6.CrossRefGoogle ScholarPubMed
Young, A. S., Brown, C. G. D., Burridge, M. J., Grootenhuis, J. G., Kanhai, G. K., Purnell, R. E. & Stagg, D. A. (1978). The incidence of theilerial parasites in East African buffalo (Syncerus caffer). Zeitschrift für Tropenmedizin und Parasitologie 29, 281–8.Google ScholarPubMed
Young, A. S., Mutugi, J. J., Kariuki, D. P., Lampard, D., Maritim, A. C., Ngumi, P. N., Linyoni, A., Leitch, B. L., Ndungu, S. G., Lesan, A. C., Mining, S. K., Grootenhuis, J. G., Orinda, G. O. & Wesonga, D. (1992). Immunisation of cattle against theileriosis in Nakuru District of Kenya by infection and treatment and the introduction of unconventional tick control. Veterinary Parasitology 42, 225–40.CrossRefGoogle ScholarPubMed