Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-13T01:50:52.875Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of the synthetic plant-growth substance, 2, 4-dichlorophenoxyacetic acid, on the host–parasite relationships of some plant-parasitic nematodes in monoxenic callus culture

Published online by Cambridge University Press:  06 April 2009

J. M. Webster  and
D. Lowe
J. M. Webster
Affiliation:
Nematology Department, Rothamsted Experimental Station, Harpenden
D. Lowe
Affiliation:
Nematology Department, Rothamsted Experimental Station, Harpenden
Get access Rights & Permissions [Opens in a new window]

Extract

Some callus tissues induced by 2,4–D and derived from plants resistant to plant-parasitic nematodes lost their resistance to the nematodes. Red clover callus supported a large population of A. ritzemabosi, whereas red clover seedlings did not. Six races of D. dipsaci were cultured on lucerne and red clover callus tissues and reproduced rapidly, although races usually multiplied more on callus from susceptible than from resistant plants. A. ritzemabosi, normally only a foliage parasite, reproduced equally well in stem and root callus. H. rostochiensis did not reproduce in callus culture.

Nematodes multiplied most in callus that grew fastest; both reproduction of A. ritzemabosi and the growth of callus were greatest with 0.125 mg/1 of 2,4–D. Reproduction was inhibited by 5.0 mg/100 ml of 2,4–D. 2,4–D influenced nematode reproduction indirectly by making callus, which provides a better environment for nematode feeding and reproduction.

Nematode extracts added to an agar medium caused callus formation on red clover seedlings and nematodes feeding on these tissues reproduced faster than on normal seedlings. Hence, the substances secreted by a nematode into a plant may act on the tissues in a manner similar to 2,4–D. The host–parasite relationship is probably partially controlled by the host's plant-growth substances and the effect on them of the nematode's secretions.

We thank Mr C. T. Drakes for assistance, and Mr K. Smith of Imperial Chemical Industries for advice with the initial callus culture.

Type
Research Article
Information
Parasitology , Volume 56 , Issue 2 , May 1966 , pp. 313 - 322
Copyright
Copyright © Cambridge University Press 1966

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Audus, L. J. (1959). Plant Growth Substances. London: Leonard Hill (Books) Ltd.Google Scholar
Dolliver, J. S., Hildebrandt, A. C. & Riker, A. J. (1962). Studies of reproduction of Aphelenchoides ritzemabosi (Schwartz) on plant tissues in culture. Nematologica, 7, 294–300.Google Scholar
Hildebrandt, A. C., Riker, A. J. & Duggar, B. M. (1946). The influence of the composition of the medium on growth in vitro of excised tobacco and sunflower tissue cultures. Am. J Bot. 33, 591–97.CrossRefGoogle Scholar
Jones, F. G. W. & Pawelska, K. (1963). The behaviour of populations of potato-root eelworm (Heterodera rostochiensis Woll.) towards some resistant tuberous and other Solanum species. Ann. appl. Biol. 51, 277–94.CrossRefGoogle Scholar
Kassanis, B., Tinsley, T. W. & Quak, F. (1958). The inoculation of tobacco callus tissue with tobacco mosaic virus. Ann. appl. Biol. 46, 11–19.CrossRefGoogle Scholar
Krusberg, L. R. (1961). Studies on the culturing and parasitism of plant parasitic nematodes, in particular Ditylenchus dipsaci and Aphelenchoides ritzemabosi on alfalfa tissues. Nematologica, 6, 181–200.CrossRefGoogle Scholar
Krusberg, L. R. (1962). Biology of plant-parasitic nematodes. J. Parasit. 48, 826–29.CrossRefGoogle ScholarPubMed
Krusberg, L. R. & Blickenstaff, M. L. (1964). Influence of plant growth regulating substances on reproduction of Ditylenchus dipsaci, Pratylenchus penetrans and Pratylenchuszeae on alfalfa tissue cultures. Nematologica, 10, 145–50.CrossRefGoogle Scholar
Lingappa, Y. (1957). Tissue cultures of Solanum tuberosum and Ipomoea pandurata. Am. J. Bot. 44, 419–23.CrossRefGoogle Scholar
Oostenbrink, M. (1954). Een doelmatige methode voor het toetsen van aaltjesbestrijdings-middelen in grond met Hoplolaimus uniformis als proefdier. Meded. LandbHoogesch. OpzoekStns Gent, 19, 377–408.Google Scholar
Palo, A. V. (1962). Translocation and development of stem eelworm, Ditylenchus dipsaci (Kühn), in lucerne, Medicago sativa L. Nematologica, 7, 122–32.CrossRefGoogle Scholar
Peacock, F. C. (1959). The development of a technique for studying the host/parasite relationship of the root-knot nematode Meloidogyne incognita under controlled conditions. Nematologica, 4, 43–55.CrossRefGoogle Scholar
Sandstedt, R. & Schuster, M. L. (1963). Nematode-induced callus on carrot discs grown in vitro. Phytopathology, 53, 1309–12.Google Scholar
Seinhorst, J. W. (1956). Biologische rassen van het stengelaaltje Ditylenchus dipsaci (Kühn) Filipjev on hun waardplanten. 1. Reacties van vatbare en resistente planten op aantasting en verschillende vormen van resistentie. Tijdschr. PIZiekt. 62, 179–88.Google Scholar
Webster, J. M. (1962). The quantitative extraction of Ditylenchus dipsaci (Kühn) from plant tissues by a modified Seinhorst mistifier. Nematologica, 8, 245–51.CrossRefGoogle Scholar
Webster, J. M. (1964). Biological races in species of plant parasitic nematodes. Parasitology, 54, 8P.Google Scholar
Yu, P. K. & Viglierchio, D. R. (1964). Plant growth substances and parasitic nematodes. 1. Root-knot nematodes and tomato. Expl Parasit. 15, 242–48.CrossRefGoogle Scholar