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Genome sizes of the entomopathogenic nematodes Steinernema carpocapsae and Heterorhabditis bacteriophora (Nematoda: Rhabditida)

Published online by Cambridge University Press:  01 May 1997

E. GRENIER ,
F. M. CATZEFLIS  and
P. ABAD
E. GRENIER
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, 123 bd Francis Meilland, BP 2078, 06606 Antibes cedex, France
F. M. CATZEFLIS
Affiliation:
Laboratoire de Paléontologie, ISEM – UMR 5554 CNRS, Université Montpellier II, place Eugène Bataillon, 34095 Montpellier cedex 5, France
P. ABAD
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, 123 bd Francis Meilland, BP 2078, 06606 Antibes cedex, France
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Abstract

There is currently no information on the genome size and complexity in the entomoparasitic nematodes Steinernema and Heterorhabditis. DNA reassociation kinetics were used to determine the genome size and complexity in 2 species: Steinernema carpocapsae and Heterorhabditis bacteriophora. C0t curves derived from renaturation kinetics of denatured DNA indicate that the genomes of these entomoparasitic rhabditids are different both in size and complexity. Genome sizes were estimated at 2·3 × 108 bp for S. carpocapsae and 3·9 × 107 bp for H. bacteriophora and repetitive DNA contents were found to represent 39% and 51% of these respective genomes.

Keywords

Steinernema carpocapsaeHeterorhabditis bacteriophoraDNA complexityC0t curves
Type
Research Article
Information
Parasitology , Volume 114 , Issue 5 , May 1997 , pp. 497 - 501
Copyright
1997 Cambridge University Press

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