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High prevalence of Schistosoma haematobium × Schistosoma bovis hybrids in schoolchildren in Côte d'Ivoire

Published online by Cambridge University Press:  21 November 2019

Etienne K. Angora Open the ORCID record for Etienne K. Angora [Opens in a new window] ,
Jean-François Allienne ,
Olivier Rey ,
Hervé Menan ,
André O. Touré ,
Jean T. Coulibaly ,
Giovanna Raso ,
William Yavo ,
Eliézer K. N'Goran  and
Jürg Utzinger
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Etienne K. Angora
Affiliation:
Swiss Tropical and Public Health Institute, Basel, Switzerland University of Basel, Basel, Switzerland Unité de Formation et de Recherche Sciences Pharmaceutiques et Biologiques, Université Félix Houphouët-Boigny, Abidjan, Côte d'Ivoire
Jean-François Allienne
Affiliation:
IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Perpignan, France
Olivier Rey
Affiliation:
IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Perpignan, France
Hervé Menan
Affiliation:
Unité de Formation et de Recherche Sciences Pharmaceutiques et Biologiques, Université Félix Houphouët-Boigny, Abidjan, Côte d'Ivoire
André O. Touré
Affiliation:
Institut Pasteur de Côte d'Ivoire, Abidjan, Côte d'Ivoire
Jean T. Coulibaly
Affiliation:
Swiss Tropical and Public Health Institute, Basel, Switzerland University of Basel, Basel, Switzerland Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d'Ivoire Centre Suisse de Recherches Scientifiques en Côte d'Ivoire, Abidjan, Côte d'Ivoire
Giovanna Raso
Affiliation:
Swiss Tropical and Public Health Institute, Basel, Switzerland University of Basel, Basel, Switzerland
William Yavo
Affiliation:
Unité de Formation et de Recherche Sciences Pharmaceutiques et Biologiques, Université Félix Houphouët-Boigny, Abidjan, Côte d'Ivoire
Eliézer K. N'Goran
Affiliation:
Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d'Ivoire Centre Suisse de Recherches Scientifiques en Côte d'Ivoire, Abidjan, Côte d'Ivoire
Jürg Utzinger
Affiliation:
Swiss Tropical and Public Health Institute, Basel, Switzerland University of Basel, Basel, Switzerland
Oliver Balmer
Affiliation:
Swiss Tropical and Public Health Institute, Basel, Switzerland University of Basel, Basel, Switzerland
Jérôme Boissier*
Affiliation:
IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Perpignan, France
*
Author for correspondence: Jérôme Boissier, E-mail: boissier@univ-perp.fr
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Abstract

Schistosomiasis is a neglected tropical disease, though it is highly prevalent in many parts of sub-Saharan Africa. While Schistosoma haematobium-bovis hybrids have been reported in West Africa, no data about Schistosoma hybrids in humans are available from Côte d'Ivoire. This study aimed to identify and quantify S. haematobium-bovis hybrids among schoolchildren in four localities of Côte d'Ivoire. Urine samples were collected and examined by filtration to detect Schistosoma eggs. Eggs were hatched and 503 miracidia were individually collected and stored on Whatman® FTA cards for molecular analysis. Individual miracidia were molecularly characterized by analysis of mitochondrial cox1 and nuclear internal transcribed spacer 2 (ITS 2) DNA regions. A mitochondrial cox1-based diagnostic polymerase chain reaction was performed on 459 miracidia, with 239 (52.1%) exhibiting the typical band for S. haematobium and 220 (47.9%) the S. bovis band. The cox1 and ITS 2 amplicons were Sanger sequenced from 40 randomly selected miracidia to confirm species and hybrids status. Among the 33 cox1 sequences analysed, we identified 15 S. haematobium sequences (45.5%) belonging to seven haplotypes and 18 S. bovis sequences (54.5%) belonging to 12 haplotypes. Of 40 ITS 2 sequences analysed, 31 (77.5%) were assigned to pure S. haematobium, four (10.0%) to pure S. bovis and five (12.5%) to S. haematobium-bovis hybrids. Our findings suggest that S. haematobium-bovis hybrids are common in Côte d'Ivoire. Hence, intense prospection of domestic and wild animals is warranted to determine whether zoonotic transmission occurs.

Keywords

Côte d'Ivoirehybridsmolecular analysisSchistosoma bovisSchistosoma haematobium
Type
Research Article
Information
Parasitology , Volume 147 , Issue 3 , March 2020 , pp. 287 - 294
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Copyright
Copyright © Cambridge University Press 2019

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Footnotes

*

These authors contributed equally to this work.

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