Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-13T02:26:40.820Z Has data issue: false hasContentIssue false
  • English
  • Français

The Hybrid II assay: a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle

Published online by Cambridge University Press:  09 September 2011

RONEL PIENAAR ,
FRED T. POTGIETER ,
ABDALLA A. LATIF ,
ORIEL M. M. THEKISOE  and
BEN J. MANS
RONEL PIENAAR
Affiliation:
Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa Parasitology Research Program, Department of Zoology and Entomology, University of the Free State Qwaqwa Campus, Private Bag X13, Phuthaditjhaba, 9866, South Africa
FRED T. POTGIETER
Affiliation:
Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa
ABDALLA A. LATIF
Affiliation:
Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa The Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South Africa
ORIEL M. M. THEKISOE
Affiliation:
Parasitology Research Program, Department of Zoology and Entomology, University of the Free State Qwaqwa Campus, Private Bag X13, Phuthaditjhaba, 9866, South Africa
BEN J. MANS*
Affiliation:
Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa The Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South Africa
*
*Corresponding author: Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort 0110, South Africa. Tel: +27 12 5299200. Fax: +27 12 5299434. E-mail: mansb@arc.agric.za
Get access Rights & Permissions [Opens in a new window]

Summary

Corridor disease is an acute, fatal disease of cattle caused by buffalo-adapted Theileria parva. This is a nationally controlled disease in South Africa and strict control measures apply for the movement of buffalo, which includes mandatory testing for the presence of T. parva and other controlled diseases. Accurate diagnosis of the T. parva carrier state in buffalo using the official real-time hybridization PCR assay (Sibeko et al. 2008), has been shown to be affected by concurrent infection with T. sp. (buffalo)-like parasites. We describe the Hybrid II assay, a real-time hybridization PCR method, which compares well with the official hybridization assay in terms of specificity and sensitivity. It is, however, not influenced by mixed infections of T. sp. (buffalo)-like parasites and is as such a significant improvement on the current hybridization assay.

Keywords

Corridor diseasediagnostic assayreal-time hybridizationSyncerus cafferTheileria parva
Type
Research Article
Information
Parasitology , Volume 138 , Issue 14 , December 2011 , pp. 1935 - 1944
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Allsopp, M. T., Theron, J., Coetzee, M. L., Dunsterville, M. T. and Allsopp, B. A. (1999). The occurrence of Theileria and Cowdria parasites in African buffalo (Syncerus caffer) and their associated Amblyomma hebraeum ticks. Onderstepoort Journal of Veterinary Research 66, 245–249.Google ScholarPubMed
Barnett, S. F. and Brocklesby, D. W. (1966). The passage of “Theileria lawrencei (Kenya)” through cattle. British Veterinary Journal 122, 396–409.CrossRefGoogle Scholar
Barratt, K. and Mackay, J. F. (2002). Improving real-time PCR genotyping assays by asymmetric amplification. Journal of Clinical Microbiology 40, 1571–1572.CrossRefGoogle ScholarPubMed
Burridge, M. J. and Kimber, C. D. (1972). The indirect fluorescent antibody test for experimental East Coast fever (Theileria parva infection of cattle). Evaluation of a cell culture schizont antigen. Research in Veterinary Science 13, 451–455.CrossRefGoogle ScholarPubMed
Collins, N. E., Allsopp, M. T. and Allsopp, B. A. (2002). Molecular diagnosis of theileriosis and heartwater in bovines in Africa. Transactions of the Royal Society of Tropical Medicine and Hygiene 96, S217–S224.CrossRefGoogle ScholarPubMed
De Vos, A. J. and Potgieter, F. T. (1983). The effect of tick control on the epidemiology of bovine babesiosis. Onderstepoort Journal of Veterinary Research 50, 3–5.Google ScholarPubMed
Estrada-Peña, A. (2003). Climate change decreases habitat suitability for some tick species (Acari: Ixodidae) in South Africa. Onderstepoort Journal of Veterinary Research 70, 79–93.Google ScholarPubMed
Gubbels, J. M., de Vos, A. P., van der Weide, M., Viseras, J., Schouls, L. M., de Vries, E. and Jongejan, F. (1999). Simultaneous detection of bovine Theileria and Babesia species by reverse line blot hybridization. Journal of Clinical Microbiology 37, 1782–1789.CrossRefGoogle ScholarPubMed
Koch, H. T., Norval, R. A. I., Ocama, J. G. R. and Munatswa, F. C. (1992). A study on Theileria parva bovis carrier state. Preventive Veterinary Medicine 12, 197–203.CrossRefGoogle Scholar
Lawrence, J. A. (1979). The differential diagnosis of the bovine theilerias of southern Africa. Journal of the South African Veterinary Association 50, 311–313.Google ScholarPubMed
Lawrence, J. A., Norval, R. A. and Uilenberg, G. (1983). Rhipicephalus zambeziensis as a vector of bovine Theileriae. Tropical Animal Health and Production 15, 39–42.CrossRefGoogle ScholarPubMed
Mamabolo, M. V., Ntantiso, L., Latif, A. and Majiwa, P. A. (2009). Natural infection of cattle and tsetse flies in South Africa with two genotypic groups of Trypanosoma congolense. Parasitology 136, 425–431.CrossRefGoogle ScholarPubMed
Mans, B. J., Pienaar, R., Latif, A. A. and Potgieter, F. T. (2011). Diversity in the 18S SSU rRNA V4 hyper-variable region of Theileria in bovines and African buffalo (Syncerus caffer) from southern Africa. Parasitology 138, 766–779.CrossRefGoogle Scholar
Neitz, W. O. (1957). Theileriosis, gonderiosis and cytauxzoonoses: a review. Onderstepoort Journal of Veterinary Research 27, 275–430.Google Scholar
Neitz, W. O. (1958). Can Corridor disease-recovered cattle serve as reservoirs of Gonderia lawrencei? Bulletin of Epizootic Diseases of Africa 6, 151–154.Google Scholar
Neitz, W. O., Canham, A. S. and Kluge, E. (1955). Corridor disease: A fatal form of bovine theileriosis encountered in Zululand. Journal of the South African Veterinary Association 26, 79–87.Google Scholar
Norval, R. A., Lawrence, J. A., Young, A. S., Perry, B. D., Dolan, T. T. and Scott, J. (1991). Theileria parva: influence of vector, parasite and host relationships on the epidemiology of theileriosis in southern Africa. Parasitology 102, 347–356.CrossRefGoogle ScholarPubMed
Norval, R. A. I., Perry, B. D., Young, A. S. (1992). The Epidemiology of Theileriosis in Africa. Academic Press, London, UK.Google Scholar
Odongo, D. O., Sunter, J. D., Kiara, H. K., Skilton, R. A. and Bishop, R. P. (2010). A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals. Parasitology Research 106, 357–365.CrossRefGoogle ScholarPubMed
Papli, N., Landt, O., Fleischer, C., Koekemoer, J. O., Mans, B. J., Pienaar, R., Josemans, A., Zweygarth, E., Potgieter, F. and Latif, A. A. (2011). Evaluation of a TaqMan real-time PCR for the detection of Theileria parva in buffalo and cattle. Veterinary Parasitology 175, 356–359.CrossRefGoogle Scholar
Pfaffl, M. W. (2004). Quantification strategies in real-time PCR. In A-Z of Quantitative PCR (ed. Bustin, S. A.), pp. 87–112. International University Line (IUL), La Jolla, CA, USA.Google Scholar
Pienaar, R., Potgieter, F. T., Latif, A. A., Thekisoe, O. M. M. and Mans, B. J. (2011). Mixed Theileria infections in free-ranging buffalo herds: Implications for diagnosing Theileria parva infections in Cape buffalo (Syncerus caffer). Parasitology 138, 884–895. doi: 10.1017/S0031182011000187.CrossRefGoogle ScholarPubMed
Potgieter, F. T., Stoltsz, W. H., Blouin, E. F., Roos, J. A. (1988). Corridor disease in South Africa: a review of the current status. Journal of the South African Veterinary Association 59, 155–160.Google ScholarPubMed
Sibeko, K. P., Geysen, D., Oosthuizen, M. C., Matthee, C. A., Troskie, M., Potgieter, F. T., Coetzer, J. A. and Collins, N. E. (2010). Four p67 alleles identified in South African Theileria parva field samples. Veterinary Parasitology 167, 244–254.CrossRefGoogle ScholarPubMed
Sibeko, K. P., Oosthuizen, M. C., Collins, N. E., Geysen, D., Rambritch, N. E., Latif, A. A., Groeneveld, H. T., Potgieter, F. T. and Coetzer, J. A. (2008). Development and evaluation of a real-time polymerase chain reaction test for the detection of Theileria parva infections in Cape buffalo (Syncerus caffer) and cattle. Veterinary Parasitology 155, 37–48.CrossRefGoogle ScholarPubMed
Stoltsz, W. H. (1989). Theileriosis in South Africa: A brief review. Revue Scientifique et Technique de L Office International des Épizooties 8, 93–102.CrossRefGoogle ScholarPubMed
Theiler, A. (1904). Rhodesian tick fever. Transvaal Agricultural Journal 2, 421–438.Google Scholar
Thekisoe, O. M., Rambritch, N. E., Nakao, R., Bazie, R. S., Mbati, P., Namangala, B., Malele, I., Skilton, R. A., Jongejan, F., Sugimoto, C., Kawazu, S. and Inoue, N. (2010). Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes. International Journal for Parasitology 40, 55–61.CrossRefGoogle ScholarPubMed
Thompson, B. E., Latif, A. A., Oosthuizen, M. C., Troskie, M. and Penzhorn, B. L. (2008). Occurrence of Theileria parva infection in cattle on a farm in the Ladysmith district, KwaZulu-Natal, South Africa. Journal of the South African Veterinary Association 79, 31–35.CrossRefGoogle ScholarPubMed
Uilenberg, G. (1976). Tick-borne livestock diseases and their vectors. 2. Epizootiology of tick-borne diseases. World Animal Review 17, 8–15.Google Scholar
Walker, J. B., Norval, R. A. and Corwin, M. D. (1981). Rhipicephalus zambeziensis sp. Nov., a new tick from eastern and southern africa, together with a redescription of Rhipicephalus appendiculatus Neumann, 1901 (Acarina, Ixodidae). Onderstepoort Journal of Veterinary Research 48, 87–104.Google Scholar
Young, A. S., Leitch, B. L., Newson, R. M. and Cunningham, M. P. (1986). Maintenance of Theileria parva parva infection in an endemic area of Kenya. Parasitology 93, 9–16.CrossRefGoogle Scholar
Yusufmia, S. B., Collins, N. E., Nkuna, R., Troskie, M., Van den Bossche, P. and Penzhorn, B. L. (2010). Occurrence of Theileria parva and other haemoprotozoa in cattle at the edge of Hluhluwe-iMfolozi Park, KwaZulu-Natal, South Africa. Journal of the South African Veterinary Association 81, 45–49.CrossRefGoogle ScholarPubMed