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Morphological and molecular characterization of tetraphyllidean merocercoids (Platyhelminthes: Cestoda) of striped dolphins (Stenella coeruleoalba) from the Western Mediterranean

Published online by Cambridge University Press:  18 November 2004

C. AGUSTÍ ,
F. J. AZNAR ,
P. D. OLSON ,
D. T. J. LITTLEWOOD ,
A. KOSTADINOVA  and
J. A. RAGA
C. AGUSTÍ
Affiliation:
Marine Zoology Unit, Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, P.O. Box 22085, 46071 Valencia, Spain
F. J. AZNAR
Affiliation:
Marine Zoology Unit, Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, P.O. Box 22085, 46071 Valencia, Spain
P. D. OLSON
Affiliation:
Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
D. T. J. LITTLEWOOD
Affiliation:
Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
A. KOSTADINOVA
Affiliation:
Marine Zoology Unit, Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, P.O. Box 22085, 46071 Valencia, Spain Department of Biodiversity, Central Laboratory of General Ecology, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria
J. A. RAGA
Affiliation:
Marine Zoology Unit, Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, P.O. Box 22085, 46071 Valencia, Spain
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Abstract

Two types of tetraphyllidean merocercoids, Phyllobothrium delphini and Monorygma grimaldii, are well known from most cetaceans world-wide. The role of cetaceans in the life-cycle of these merocercoids is unclear because their specific identity is as yet unknown. The problem is compounded by poor descriptions of both merocercoids. We used light and scanning electron microscopy, and histological techniques to provide a thorough description of merocercoids collected from 11 striped dolphins, Stenella coeruleoalba, from the Spanish Mediterranean. We also described, for the first time, specimens of P. delphini with immature proglottides. Our merocercoids were morphologically similar to those described previously, except in the structure of the apical organ. Intra- and inter-sample variability in the morphology of the apical organ suggested that it degenerates during larval development. A subsample of 16 specimens of P. delphini and M. grimaldii was characterized for the D2 variable region of the large subunit ribosomal RNA gene (LSU) and compared with published tetraphyllidean cestode LSU sequences. P. delphini showed 2 unique signatures that differed from one another by a single base, whereas all sequences of M. grimaldii were identical. This suggests that each type may represent a single species, contrary to previous speculations based on morphological data. All merocercoid specimens formed a clade together with Clistobothrium montaukensis. Based on the low degree of divergence, all specimens of this clade are predicted to be congeneric.

Keywords

TetraphyllideamerocercoidPhyllobothrium delphiniMonorygma grimaldiiClistobothrium montaukensisstriped dolphinmolecular diagnostics
Type
Research Article
Information
Parasitology , Volume 130 , Issue 4 , April 2005 , pp. 461 - 474
Copyright
2005 Cambridge University Press

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