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Parasite-induced oxidative stress in liver tissue of fathead minnows exposed to trematode cercariae

Published online by Cambridge University Press:  16 August 2012

A. D. STUMBO ,
C. P. GOATER  and
A. HONTELA
A. D. STUMBO*
Affiliation:
Department of Biological Sciences, Water Institute for Sustainable Environments (WISE), University of Lethbridge, 4401 University Drive West, Lethbridge, AB, CanadaT1 K 3M4
C. P. GOATER
Affiliation:
Department of Biological Sciences, Water Institute for Sustainable Environments (WISE), University of Lethbridge, 4401 University Drive West, Lethbridge, AB, CanadaT1 K 3M4
A. HONTELA
Affiliation:
Department of Biological Sciences, Water Institute for Sustainable Environments (WISE), University of Lethbridge, 4401 University Drive West, Lethbridge, AB, CanadaT1 K 3M4
*
*Corresponding author: Tel:+403 329 2319. E-mail:anthony.stumbo@uleth.ca
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Summary

Although results from field surveys have linked parasites to oxidative stress in their fish hosts, direct evidence involving experimentally infected hosts is lacking. We evaluated the effects of experimental infections with larval trematodes on induction of oxidative stress in fathead minnows, Pimephales promelas. Juvenile fish were exposed in the laboratory to the larvae (cercariae) of 2 species of trematode: Ornithodiplostomum sp. that develops in the liver, and O. ptychocheilus that develops in the brain. For Ornithodiplostomum sp., lipid peroxidation concentration in liver tissue increased 5 days after exposure and remained higher than controls until the end of the experiment at 28 days. For O. ptychocheilus, liver lipid peroxidation concentration was higher than controls at 5 days, but not thereafter. Sustained elevation in lipid peroxidation concentration for the liver trematode may be explained by direct tissue damage caused by developing larvae in the liver, or by an immune response. These experimental results support those from field studies, indicating that the lipid peroxidation assay may be an effective biomonitor for parasite-induced oxidative stress in fish, and that the nature of the oxidative stress response is species and/or tissue specific.

Keywords

lipid peroxidationmetacercariaePimephales promelas
Type
Research Article
Information
Parasitology , Volume 139 , Issue 12 , October 2012 , pp. 1666 - 1671
Copyright
Copyright © Cambridge University Press 2012

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