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Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens

Published online by Cambridge University Press:  29 November 2016

M. A. CORRAL ,
F. M. PAULA ,
D. M. C. L. MEISEL ,
V. L. P. CASTILHO ,
E. M. N. GONÇALVES ,
D. LEVY ,
S. P. BYDLOWSKI ,
P. P. CHIEFFI ,
W. CASTRO-BORGES  and
R. C. B. GRYSCHEK
M. A. CORRAL
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, SP, Brazil
F. M. PAULA
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, SP, Brazil Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo SP, Brazil
D. M. C. L. MEISEL
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, SP, Brazil
V. L. P. CASTILHO
Affiliation:
Divisão de Laboratório Central, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil
E. M. N. GONÇALVES
Affiliation:
Divisão de Laboratório Central, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil
D. LEVY
Affiliation:
Laboratório de Investigação Médica (LIM-31), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil
S. P. BYDLOWSKI
Affiliation:
Laboratório de Investigação Médica (LIM-31), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil
P. P. CHIEFFI
Affiliation:
Faculdade de Ciências Médicas, Santa Casa, São Paulo, SP, Brazil
W. CASTRO-BORGES
Affiliation:
Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil
R. C. B. GRYSCHEK*
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, SP, Brazil Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo SP, Brazil
*
*Corresponding author: Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, USP, São Paulo. Instituto de Medicina Tropical, prédio II, 2° andar, Av. Dr Enéas de Carvalho Aguiar, 470, Cerqueira Cesar, 05403-000, São Paulo, SP, Brazil. E-mail: rcbgry@usp.br
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Summary

Strongyloides venezuelensis is a parasitic nematode of rodents that is frequently used to obtain heterologous antigens for immunological diagnosis of human strongyloidiasis. The aim of this study was to identify antigens from filariform larvae of S. venezuelensis for immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from filariform larvae of S. venezuelensis were obtained in phosphate saline (SS and SM) and in Tris–HCl buffer (TS and TM), and were analysed by Western blotting. Different antigenic components were recognized by IgG antibodies from the sera of strongyloidiasis patients. Highest recognition was observed for a 30–40 kDa mass range present in all antigenic fractions. The band encompassing this mass range was then excised and subjected to mass spectrometry for protein identification. Immunoreactive proteins identified in the soluble fractions corresponded to metabolic enzymes, whereas cytoskeletal proteins and galectins were more abundant in the membrane fractions. These results represent the first approach towards identification of S. venezuelensis antigens for use in immunodiagnostic assays for human strongyloidiasis.

Keywords

Strongyloides venezuelensisimmunodiagnosticantigensactingalectin
Type
Research Article
Information
Parasitology , Volume 144 , Issue 2 , February 2017 , pp. 124 - 130
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Copyright
Copyright © Cambridge University Press 2016 

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