Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-19T19:37:46.819Z Has data issue: false hasContentIssue false
  • English
  • Français

A rapid techinque for the recovery of strongyloid infective larvae from pasture and soil samples

Published online by Cambridge University Press:  06 April 2009

R. R. Young  and
A. C. Trajstman
R. R. Young
Affiliation:
CSIRO Division of Animal Health,*Animal Health Research Laboratory, Private Bag No. 1, P.O., Parkville, Victoria 3052, Australia
A. C. Trajstman
Affiliation:
CSIRO Division of Mathematics and Statistics, Private Bag No. 1, P.O., Parkville, Victoria 3052, Australia
Get access Rights & Permissions [Opens in a new window]

Summary

A technique is described for the recovery of infective larvae of strongyloid nematodes from sediment resulting from the washing of pasture and soil samples. The process of recovery and counting of larvae is described by a model based on the binomial distribution. Tests of the technique have shown that it will recover approximately 50% of the larvae present in samples of pasture sediment of either 7·5 or 12·5 ml in volume after centrifugation. The efficiency of the technique was independent of the number of larvae present in samples of pasture sediment. The technique was shown to be less efficient and more variable in recovery of larvae from samples of soil sediment. Estimations in efficiency of recovery from soil sediment ranged from 64 to 110·2% and a significant effect of volume of sediment on recovery was detected.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 3 , June 1980 , pp. 425 - 431
Copyright
Copyright © Cambridge University Press 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bawden, R. J. (1969). A rapid technique for the recovery of strongyloid larvae from pasture samples. Australian Veterinary Journal 45, 228–30.Google ScholarPubMed
Crofton, H. D. (1954). The ecology of the immature phases of trichostrongyle parasites. V. The estimation of pasture infestation. Parasitology 44, 313–24.CrossRefGoogle ScholarPubMed
Donald, A. D. (1967). A technique for the recovery of strongyloid infective larvae from small sample units for pasture. Journal of Helminthology 41, 1–10.CrossRefGoogle Scholar
Durie, P. H. (1959). A technique for the recovery of infective strongyle larvae from soil and pasture. Journal of Helminthology 33, 189–96.CrossRefGoogle ScholarPubMed
Heath, D. D. & Major, G. W. (1968). A technique for the recovery of strongyle larvae from masticated herbage. Journal of Helminthology 42, 299–304.CrossRefGoogle ScholarPubMed
Heip, C., Smol, N. & Hautekiet, W. (1974). A rapid method of extracting meiobenthic nematodes and copepods from mud and detritus. Marine Biology 28, 79–81.CrossRefGoogle Scholar
Lancaster, M. B. (1970). The recovery of infective nematode larvae from herbage samples. Journal of Helminthology 44, 219–30.CrossRefGoogle Scholar
Parfitt, J. W. (1955). Two techniques used for the detection and enumeration of the larvae of Dictyocaulus viviparus in faeces and herbage. Laboratory Practice 4, 15–16.Google Scholar
Smeal, M. G. & Hendy, G. A. (1972). A technique for the recovery of strongyloid infective larvae from pasture. Journal of Helminthology 46, 201–11.CrossRefGoogle ScholarPubMed
Taylor, E. L. (1939). Technique for the estimation of pasture infestation by strongyloid larvae. Parasitology 31, 473–8.CrossRefGoogle Scholar