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Role of reactive oxygen species in expulsion of Nippostrongylus brasiliensis from rats

Published online by Cambridge University Press:  06 April 2009

S. Batra ,
J. K. Srivastrava ,
S. Gupta ,
J. C. Katiyar  and
V. M. L. Srivastava
S. Batra
Affiliation:
Divisions of Biochemistry, Central Drug Research Institute, Lucknow-226001, India
J. K. Srivastrava
Affiliation:
Divisions of Parasitology, Central Drug Research Institute, Lucknow-226001, India
S. Gupta
Affiliation:
Divisions of Parasitology, Central Drug Research Institute, Lucknow-226001, India
J. C. Katiyar
Affiliation:
Divisions of Parasitology, Central Drug Research Institute, Lucknow-226001, India
V. M. L. Srivastava*
Affiliation:
Divisions of Biochemistry, Central Drug Research Institute, Lucknow-226001, India
*
*Reprint requests to Dr V. M. L. Srivastava, Division of Biochemistry, Central Drug Research Institute, Lucknow-226001, India.
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Summary

To understand the mechanism for the expulsion of Nippostrongylus brasiliensis from rats, age-dependent variations in the metabolism of reactive oxygen species in the parasite and the host intestines were examined. N. brasiliensis showed an age-dependent increase in its susceptibility to xanthine-xanthine oxidase and t−butyl hydroperoxide generated oxidants as well as to H2O2. Protection obtained with several scavengers suggested that the worms were damaged by the combined action of oxidants generated by the in vitro systems employed. The level of superoxide dismutase in the nematode and its release into the surroundings exhibited a marked depression with advancement of age. No such alteration was, however, recorded for catalase and glutathione peroxidase. An appreciable decrease in the level of reduced glutathione in older N. brasiliensis appears to render them prone to oxidant attack. The rat intestines, on the other hand, exhibited an appreciable depression in catalase and a reduced glutathione content with progress of the infection. Vitamin E levels were elevated. The release of O27 and H2O2 by the intestines was also found to be greater during later stages of the infection. The combined effect of the changes observed in N. brasiliensis and in the rat intestines may be at least partly responsible for expulsion of the nematode from the rats after day 10.

Keywords

Nippostrongylus brasiliensisreactive oxygen speciessuperoxide dismutasevitamin Eglutathione.
Type
Research Article
Information
Parasitology , Volume 106 , Issue 2 , February 1993 , pp. 185 - 192
Copyright
Copyright © Cambridge University Press 1993

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