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Schistosoma mansoni: signal transduction processes during the development of the reproductive organs

Published online by Cambridge University Press:  18 February 2010

S. BECKMANN ,
T. QUACK ,
C. BURMEISTER ,
C. BURO ,
T. LONG ,
C. DISSOUS  and
C. G. GREVELDING
S. BECKMANN
Affiliation:
Institute for Parasitology, Justus-Liebig-University, 35392Giessen, Germany
T. QUACK
Affiliation:
Institute for Parasitology, Justus-Liebig-University, 35392Giessen, Germany
C. BURMEISTER
Affiliation:
Institute for Parasitology, Justus-Liebig-University, 35392Giessen, Germany
C. BURO
Affiliation:
Institute for Parasitology, Justus-Liebig-University, 35392Giessen, Germany
T. LONG
Affiliation:
Unité Inserm 547, Institut Pasteur, 1 rue du Pr Calmette, 59019LILLECedex, France
C. DISSOUS
Affiliation:
Unité Inserm 547, Institut Pasteur, 1 rue du Pr Calmette, 59019LILLECedex, France
C. G. GREVELDING*
Affiliation:
Institute for Parasitology, Justus-Liebig-University, 35392Giessen, Germany
*
*Corresponding author: Christoph G. Grevelding, Justus-Liebig-University Giessen, Institute for Parasitology, Rudolf-Buchheim-Str. 2, 35392Giessen, Germany. Fax: +49 641 99 38469. E-mail: Christoph.Grevelding@vetmed.uni-giessen.de
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Summary

Among the topics of considerable interest concerning our understanding of the unusual biology of schistosomes is the sexual maturation of the female. The identification of genes coding for signal transduction proteins controlling essential steps of the pairing-dependent differentiation of the reproductive organs, vitellarium and ovary will help to substantiate our knowledge about this unique parasite. Furthermore, such signalling proteins could be potential targets to interfere with the development of this parasite to combat schistosomiasis since its pathology is caused by the eggs. This review summarises first post-genomic steps to elucidate the function of gonad-specific signalling molecules which were identified by homology-based cloning strategies, by in silico identification or by yeast two-hybrid interaction analyses, using a combination of novel techniques. These include the in vitro culture of adult schistosomes, their treatment with chemical inhibitors to block enzyme activity, the use of RNAi to silence gene function post-transcriptionally, and confocal laser scanning microscopy to study the morphological consequences of these experimental approaches. Finally, we propose a first model of protein networks that are active in the ovary regulating mitogenic activity and differentiation. Some of these molecules are also active in the testes of males, probably fulfilling similar roles as in the ovary.

Keywords

Schistosoma mansonimale-female interactionvitellariumovarytestessignal transductiongonad morphology
Type
Research Article
Information
Parasitology , Volume 137 , Special Issue 3: Development and applications of cestode and trematode laboratory models , March 2010 , pp. 497 - 520
Copyright
Copyright © Cambridge University Press 2010

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