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Sequence variation of the Cytochrome b gene of various human infecting members of the genus Leishmania and their phylogeny

Published online by Cambridge University Press:  06 May 2004

G. E. LUYO-ACERO ,
H. UEZATO ,
M. OSHIRO ,
K. TAKEI ,
K. KARIYA ,
K. KATAKURA ,
E. GOMEZ-LANDIRES ,
Y. HASHIGUCHI  and
S. NONAKA
G. E. LUYO-ACERO
Affiliation:
Department of Dermatology, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
H. UEZATO
Affiliation:
Department of Dermatology, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
M. OSHIRO
Affiliation:
Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
K. TAKEI
Affiliation:
Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
K. KARIYA
Affiliation:
Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
K. KATAKURA
Affiliation:
Department of Parasitology, Gunma University School of Medicine 3-39-22 Showa-machi, Maebashi 371-8511, Japan
E. GOMEZ-LANDIRES
Affiliation:
Departmento de Medicina Tropical, Facultad de Medicina, Universidad Catolica Santiago de Guayaquil 10833, Guayaquil, Ecuador
Y. HASHIGUCHI
Affiliation:
Department of Parasitology, Kochi Medical School, Nankoku city, Kochi 783-8505, Japan
S. NONAKA
Affiliation:
Department of Dermatology, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
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Abstract

The Cytochrome b (Cyt b) gene has proved to be useful for identification and classification of many mammals and plants. In order to evaluate the utility of this gene for discrimination of Leishmania parasites as well as for exploring their phylogenetic relationships, we determined the nucleotide sequences of the Cyt b gene from 13 human-infecting Leishmania species (14 strains) from the New and Old Worlds. The Cyt b genes, approximately 1080 base pairs, were found to be A/T rich, and their 5′ terminal-editing regions were highly conserved. The nucleotide sequence variation among them was enough to discriminate parasite species; 245 nucleotide positions were polymorphic and 190 positions were parsimony informative. The phylogenetic relationships based on this gene, showed good agreement with the classification of Lainson & Shaw (1987) except for the inclusion of L. (L.) major in the L. (L.) tropica complex and the placement of L. tarentolae in another genus. These data show that the Cyt b gene is useful for phylogenetic study of Leishmania parasites.

Keywords

LeishmaniaCytochrome bphylogeny
Type
Research Article
Information
Parasitology , Volume 128 , Issue 5 , May 2004 , pp. 483 - 491
Copyright
2004 Cambridge University Press

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