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studies on Entamoeba invadens Rodhain, 1934, in vitro, and its relationship to some other species of Entamoeba

Published online by Cambridge University Press:  06 April 2009

Elspeth W. McConnachie
Elspeth W. McConnachie
Affiliation:
Molteno Institute, University of Cambridge
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1. Optimum growth of E. invadens occurred in HSre + S in the presence of Bact. coli, at 24–30° C. The amoebae could not be ‘trained’ to grow above 35° C.

2. The amoebae ingested normal rat erythrocytes, but not those of chick or frog.

3. Mass encystation occurred only under conditions which favoured the growth of the amoebae, but was not dependent on an acceleration in growth rate; in certain strains, an increased production of cysts was correlated with the depletion of starch in the medium.

4. The amoebae were always killed by treatment with N/20-HCl for 1 hr. at room temperature, although usually they did not survive after treatment for only 10 min. At room temperature, the cysts survived treatment with N/10-HC1 for at least 6 days, and with 0·002% HgCl2 for at least 4 hr.

5. The amoebae of E. invadens were adversely affected by aerobic conditions.

6. The amoebae could survive for up to 35 days at 8° C. but for not more than 7 days at 37° C. The cysts survived for at least 14 days at 8° C., for 7, but not 11, days at 24° C., and for less than 7 days at 37° C.

7. It is suggested that, in view of the morphological identity of E. aulastomi, E. invadens, E. moshkovskii and E. histolytica, and the fact that the amoebae of these groups form a series growing at increasingly higher temperatures, these four organisms constitute subspecies rather than full species. The taxonomic status of E. ranarum is still open to question.

Type
Research Article
Information
Parasitology , Volume 45 , Issue 3-4 , November 1955 , pp. 452 - 481
Copyright
Copyright © Cambridge University Press 1955

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References

REFERENCES

Balamuth, W. (1951). Biological studies on Entamoeba histolytica. III. Induced encystation in several mediums, including an account of a new procedure. J. Infect. Dis. 88, 230.CrossRefGoogle ScholarPubMed
Balamuth, W. & Howard, B. (1946). Biological studies on Endamoeba histolytica. I. The growth cycle of population in a mixed bacterial flora. Amer. J. Trop. Med. 26, 771.CrossRefGoogle Scholar
Balamuth, W. & Sandza, J. G. (1944). Simple, standardized culture medium for physiological studies on Entamoeba histolytica. Proc. Soc. Exp. Biol., N.Y., 57, 161.CrossRefGoogle Scholar
Balamuth, W. & Wieboldt, M. L. (1951). Comparative growth cycles of Endamoeba histolytica with different combinations of bacteria. Amer. J. Trop. Med. 31, 192.CrossRefGoogle ScholarPubMed
Baud, C. A. & Borer, F. (1952). Caractères ultrastructuraux et classification systématique de deux amibes parasites. C.R. Soc. Biol., Paris, 146, 834.Google Scholar
Bishop, A. (1932). Entamoeba aulastomi Nöller. Cultivation, morphology and method of division; and cultivation of Hexamita sp. Parasitology, 24, 225.CrossRefGoogle Scholar
Boeck, W. C. & Drbohlav, J. (1925). The cultivation of Endamoeba histolytica. Amer. J. Hyg. 5, 371.Google Scholar
Brumpt, E. (1939). Une dysenterie ambienne expérimentale, fatale pour les Vipères, pourrant probablement ětre utilisée pour la destruction de ces Reptiles. C.R. Acad. Sci., Paris, 209, 362.Google Scholar
Chang, S. L. (1942). Studies on Entamoeba histolytica. I. Effect of hydrogen-ion concentration on encystation of E. histolytica in culture. Amer. J. Trop. Med. 22, 471.CrossRefGoogle Scholar
Chang, S. L. (1946). Studies on Entamoeba histolytica. IV. The relation of oxidation-reduction potentials to the growth, encystation and excystation of Entamoeba histolytica in culture. Parasitology, 37, 101.CrossRefGoogle Scholar
Chinn, B. D., Jacobs, L., Reardon, L. V. & Rees, C. W. (1942). The influence of the bacterial flora on the cultivation of Endamoeba histolytica. Amer. J. Trop. Med. 22, 137.CrossRefGoogle Scholar
Cleveland, L. R. & Sanders, E. P. (1930). Encystation, multiple fission without encystment, excystation, metacystic development and variation in a pure line of Entamoeba histolytica. Arch. Protistenk. 70, 223.Google Scholar
Cunha, A. M. Da & Fonseca, O. Da (1917). Sobre uma nova entameba, Entamoeba serpentis, n.sp. Brazil-Med. 31, 279.Google Scholar
Cunha, A. M. Da & Fonseca, O. Da (1918). Sobre a Entamoeba serpentis. Mem.Inst. Oswaldo Cruz, 10, 95.CrossRefGoogle Scholar
Dobell, C. (1918). Are Entamoeba histolytica and Entamoeba ranarum the same species? Parasitology, 10, 294.CrossRefGoogle Scholar
Dobell, C. (1927). Further observations and experiments on the cultivation of Entamoeba histolytica from cysts. Parasitology, 19, 288.CrossRefGoogle Scholar
Dobell, C. (1928). Researches on the intestinal protozoa of monkeys and man. I. General introduction, and II. Description of the whole life-history of Entamoeba histolytica in cultures. Parasitology, 20, 357.CrossRefGoogle Scholar
Dobell, C. (1947). An improved method for testing the action of emetine and other chemicals on Entamoeba histolytica in cultures. Ann. Soc. beige Méd. trop. (Liber jubilaris J. Rodhain), 27, Suppl., 201.Google Scholar
Dobell, C. (with Neal, R. A. & Hoabe, C. A.) (1952). Researches on the intestinal protozoa of monkeys and man. XII. Bacterial factors influencing the life history of Entamoeba histolytica in cultures. Parasitology, 42, 16.CrossRefGoogle ScholarPubMed
Dobell, C. & Laidlaw, P. P. (1926). On the cultivation of Entamoeba histolytica and some other entozoic amoebae. Parasitology, 18, 283.CrossRefGoogle Scholar
Drbohlav, J. J. (1925). Culture d'Entamoeba aulastomi Nöller, 1919. Ann. Parasit. hum. comp. 3, 367.CrossRefGoogle Scholar
Everritt, M. G. (1950). The relationship of population growth to in vitro encystation of Endamoeba histolytica. J. Parasit. 36, 586.CrossRefGoogle ScholarPubMed
Fantham, H. B. & Porter, A. (1950). The endoparasites of certain South African snakes, together with some remarks on their structure and effects on their hosts. Proc. Zool. Soc. Lond. 120, 599.CrossRefGoogle Scholar
Ferris, G. F. (1928). The Principles of Systematic Entomology. California: Stanford University Press.Google Scholar
Geiman, Q. M. & Ratcliffe, H. L. (1936). Morphology and life-cycle of an amoeba producing amoebiasis in reptiles. Parasitology, 28, 208.CrossRefGoogle Scholar
Hill, W. C. Osman & Neal, R. A. (1954). An epizootic due to Entamoeba invadens at the gardens of the Zoological Society of London. Proc. Zool. Soc. Lond. 123, 731.CrossRefGoogle Scholar
Hoare, C. A. (1943). Biological races in parasitic protozoa. Biol. Rev. 18, 137.CrossRefGoogle Scholar
Hoare, C. A. (1952). The taxonomic status of biological races in parasitic protozoa. Proc. Linn. Soc., Lond., 163, 44.CrossRefGoogle Scholar
McConnachie, E. W. (1954). The action of amoebicidal drugs on Entamoeba invadens Rodhain, 1934 in vitro. Parasitology, 44, 132.CrossRefGoogle ScholarPubMed
Neal, R. A. (1952). Experimental production of pure mixed strains of Entamoeba histolytica. Parasitology, 42, 40.CrossRefGoogle ScholarPubMed
Neal, R. A. (1953). Studies on the morphology and biology of Entamoeba moshkovskii Tshalaia, 1941. Parasitology, 43, 253.CrossRefGoogle ScholarPubMed
Paulson, M. (1932). An accurate method for the numerical determination of Endamoeba histolytica in vitro and its possible use with other intestinal protozoa; suggested clinical application. Amer. J. Trop. Med. 12, 387.CrossRefGoogle Scholar
Rao, V. Gopal (1951). Sterilization of cysts of Entamoeba histolytica by chemical disinfectants, and initiation and maintenance of pure cultures in association with single species of bacteria. Trans. Roy. Soc. Trop. Med. Hyg. 44, 593.Google ScholarPubMed
Ratcliffe, H. L. (1934). Gastric mucin as a culture medium for intestinal protozoa. Proc. Soc. Exp. Biol., N.Y., 31, 602.CrossRefGoogle Scholar
Ratcliffe, H. L. & Geiman, Q. M. (1933). Eleven cases of amoebiasis in reptiles. J. Parasit. 20, 139.Google Scholar
Ratcliffe, H. L. & Geiman, Q. M. (1934). Amebiasis in reptiles. Science, 79, 324.CrossRefGoogle ScholarPubMed
Ratcliffe, H. L. & Geiman, Q. M. (1938). Spontaneous and experimental amebic infection in reptiles. Arch. Path. 25, 160.Google Scholar
Rodhain, J. (1934). Entamoeba invadens n.sp., parasite de serpents. C.R. Soc. Biol., Paris, 117, 1195.Google Scholar
Rodhain, J. & Van Hoof, M. T. (1934). Note préliminaire sur le rôle pathogène possible d'Entamoeba invadens chez les serpents. C.R. Soc. Biol., Paris, 117, 1199.Google Scholar
Rodhain, J. & Van Hoof, M. T. (1935). Sur le rôle pathogène d'Entamoeba invadens. C.R. Soc. Biol., Paris, 118, 1646.Google Scholar
Rodhain, J. & Van Hoof, M. T. (1936). Sur le pouvoir hématophage de Entamoeba invadens. C.R. Soc. Biol., Paris, 123, 138.Google Scholar
Sanders, E. P. (1931). The life-cycle of Entamoeba ranarum, Grassi (1879). Arch. Protistenk. 74, 365.Google Scholar
Spingarn, C. L. & Edelman, M. H. (1950). The effect of rice powder on the growth of cultures of Endamoeba histolytica. Amer. J. Trop. Med. 30, 629.CrossRefGoogle ScholarPubMed