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Whole transcriptome analysis of the poultry red mite Dermanyssus gallinae (De Geer, 1778)

Published online by Cambridge University Press:  18 October 2013

SABINE SCHICHT ,
WEIHONG QI ,
LUCY POVEDA  and
CHRISTINA STRUBE
SABINE SCHICHT
Affiliation:
Institute for Parasitology, University of Veterinary Medicine Hannover, Buenteweg 17, 30559 Hannover, Germany
WEIHONG QI
Affiliation:
Functional Genomics Centre Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
LUCY POVEDA
Affiliation:
Functional Genomics Centre Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
CHRISTINA STRUBE*
Affiliation:
Institute for Parasitology, University of Veterinary Medicine Hannover, Buenteweg 17, 30559 Hannover, Germany
*
*Corresponding author: Institute for Parasitology, University of Veterinary Medicine Hannover, Buenteweg 17, 30559 Hannover, Germany. E-mail: christina.strube@tiho-hannover.de
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Summary

Although the poultry red mite Dermanyssus gallinae (De Geer, 1778) is the major parasitic pest in poultry farming causing substantial economic losses every year, nucleotide data are rare in the public databases. Therefore, de novo sequencing covering the transcriptome of D. gallinae was carried out resulting in a dataset of 232 097 singletons and 42 130 contiguous sequences (contigs) which were subsequently clustered into 24 140 isogroups consisting of 35 788 isotigs. After removal of sequences possibly originating from bacteria or the chicken host, 267 464 sequences (231 657 singletons, 56 contigs and 35 751 isotigs) remained, of which 10·3% showed homology to proteins derived from other organisms. The most significant Blast top-hit species was the mite Metaseiulus occidentalis followed by the tick Ixodes scapularis. To gain functional knowledge of D. gallinae transcripts, sequences were mapped to Gene Ontology terms, Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways and parsed to InterProScan. The transcriptome dataset provides new insights in general mite genetics and lays a foundation for future studies on stage-specific transcriptomics as well as genomic, proteomic, and metabolomic explorations and might provide new perspectives to control this parasitic mite by identifying possible drug targets or vaccine candidates. It is also worth noting that in different tested species of the class Arachnida no 28S rRNA was detectable in the rRNA profile, indicating that 28S rRNA might consists of two separate, hydrogen-bonded fragments, whose (heat-induced) disruption may led to co-migration with 18S rRNA.

Keywords

Next generation sequencing454 pyrosequencingde novo assemblytranscriptome analysisparasitic miteAcariDermanyssidae28S rRNA
Type
Research Article
Information
Parasitology , Volume 141 , Issue 3 , March 2014 , pp. 336 - 346
Copyright
Copyright © Cambridge University Press 2013 

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