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AFLP assessment of the genetic relationships among 12 Thymus taxa occurring in Portugal

Published online by Cambridge University Press:  27 July 2015

David V. da Silva ,
João M. Duarte ,
Maria G. Miguel  and
José M. Leitão
David V. da Silva
Affiliation:
Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
João M. Duarte
Affiliation:
Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
Maria G. Miguel
Affiliation:
Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
José M. Leitão*
Affiliation:
Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
*
*Corresponding author. E-mail: jleitao@ualg.pt
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Abstract

Thymus is a widely distributed genus in the Mediterranean region with several species endemic to the Iberian Peninsula. The genetic relationships among the 12 major Thymus taxa, T. albicans, T. caespititius, T. camphoratus, T. capitellatus, T. carnosus, T.lotocephalus, T. mastichina L. ssp. mastichina, T. pulegioides, T. villosus ssp. lusitanicus, T. villosus ssp. villosus, T. zygis ssp. sylvestris and T. zygis ssp. zygis, which occur in Portugal were assessed by AFLP (Amplified Fragment Length Polymorphisms) markers. A general agreement was found between the genetic relationships estimated by the AFLP markers and the accepted Thymus taxonomy based on morphological traits and essential oil content. The AFLP markers also supported suggestions for refinement of the taxonomy of this genus.

Keywords

AFLP markersgenetic relationshipsmolecular taxonomyThymeThymus
Type
Short Communications
Information
Plant Genetic Resources , Volume 15 , Issue 1 , February 2017 , pp. 89 - 92
Copyright
Copyright © NIAB 2015 

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References

Ben El Hadj Ali, I, Guetat, A and Boussaid, M (2012a) Genetic diversity of wild Thymus capitatus (Lamiaceae) in Tunisia using molecular markers. Dendrobiology 68: 89–100.Google Scholar
Ben El Hadj Ali, I, Guetat, A and Boussaid, M (2012b) Genetic diversity of North African Thymus algeriensis Boiss. & Reut. in Tunisia: population structure and implication for conservation. Dendrobiology 67: 67–77.Google Scholar
Cabrita, L, Akasoy, U, Hepaksoy, S and Leitão, J (2001) Suitability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones. Scientia Horticulturae 87: 261–273.CrossRefGoogle Scholar
Echeverrigaray, S, Agostin, G, Atti-Serfini, L, Paroul, N, Pauletti, GF and dos Santos, AC (2001) Correlation between the chemical and genetic relationships among commercial thyme cultivars. Journal of Agricultural and Food Chemistry 49: 4220–4223.CrossRefGoogle ScholarPubMed
Elisiário, P, Justo, E and Leitão, J (1999) Identification of mandarin hybrids by isozyme and RAPD analysis. Scientia Horticulturae 81: 287–299.CrossRefGoogle Scholar
Morales, R (2002) The history, botany and taxonomy of the genus Thymus . In: Stahl-Biskup, E and Sáez, F (eds) Thyme: The Genus Thymus. London: Taylor and Francis, pp. 1–43.Google Scholar
Rahimmalek, M, Bahreininejad, B, Khorrami, M and Tabatabaei, BE (2009) Genetic variability and geographic differentiation in Thymus daenensis subsp. daenensis, an endangered medicinal plant, as revealed by inter simple sequence repeat (ISSR) markers. Biochemical Genetics 47: 831–842.CrossRefGoogle ScholarPubMed
Rohlf, FG (2000) NTSYSpc Numerical Taxonomy and Multivariate Analysis System, Version 2.1. New York, USA: Exeter Software.Google Scholar
Rustaiee, AR, Yavari, A, Nazeri, V, Shokrpour, M, Sefidkon, F and Rasouli, M (2013) Genetic diversity and chemical polymorphism of some Thymus species. Chemistry and Biodiversity 10: 1088–1098.CrossRefGoogle ScholarPubMed
Salgueiro, LR (1994) Os tomilhos portugueses e os seus óleos essenciais. PhD Thesis. Universidade de Coimbra (in Portuguese). Google Scholar
Salgueiro, LR, Vila, R, Tomi, F, Figueiredo, C, Barroso, JG, Cañigueral, S, Casanova, J, Proença da Cunha, A and Adzet, T (1997) Variability of essential oils of Thymus caespititius from Portugal. Phytochemistry 45: 307–311.CrossRefGoogle Scholar
Salgueiro, LR, Vila, R, Tomàs, X, Cañigueral, S, Paiva, J, Proença da Cunha, A and Adzet, T (2000a) Essential oil composition and variability of Thymus lotocephalus and Thymus x mourae . Biochemical Systematics and Ecology 28: 457–470.CrossRefGoogle Scholar
Salgueiro, LR, Vila, R, Tomás, X, Cañigueral, S, Paiva, J, Proença da Cunha, A and Adzet, T (2000b) Chemotaxonomic study on Thymus villosus from Portugal. Biochemical Systematics and Ecology 28: 471–482.CrossRefGoogle Scholar
Sostaric, I, Liber, Z, Grdisa, M, Marin, PD, Stevanovic, ZD and Satovic, Z (2012) Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum). Flora 207: 654–661.CrossRefGoogle Scholar
Trindade, H, Costa, MM, Lima, SB, Pedro, LG, Figueiredo, AC and Barroso, JG (2009) A combined approach using RAPD, ISSR and volatile analysis for the characterization of Thymus caespititius from Flores, Corvo and Graciosa islands (Azores, Portugal). Biochemical Systematics and Ecology 37: 670–677.CrossRefGoogle Scholar
Yap, I and Nelson, RJ (1996) WinBoot: a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. In IRRI Discussion Paper Series No. 14. Manila, Philippines: IRRI.Google Scholar
Zarzuelo, A and Crespo, E (2002) The medicinal and non-medicinal use of thyme. In: Stahl-Biskup, E and Sáez, F (eds) Thyme: The Genus Thymus. London: Taylor and Francis, pp. 263–292.Google Scholar
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