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Applicability of chromosome-specific SSR wheat markers for the introgression of Triticum urartu in durum wheat breeding programmes

Published online by Cambridge University Press:  20 April 2011

C. Rodríguez-Suárez ,
M. C. Ramírez ,
A. Martín  and
S. G. Atienza
C. Rodríguez-Suárez*
Affiliation:
Instituto de Agricultura Sostenible, IAS-CSIC, Apdo. 4084, 14080Córdoba, Spain
M. C. Ramírez
Affiliation:
Instituto de Agricultura Sostenible, IAS-CSIC, Apdo. 4084, 14080Córdoba, Spain
A. Martín
Affiliation:
Instituto de Agricultura Sostenible, IAS-CSIC, Apdo. 4084, 14080Córdoba, Spain
S. G. Atienza
Affiliation:
Instituto de Agricultura Sostenible, IAS-CSIC, Apdo. 4084, 14080Córdoba, Spain
*
*Corresponding author. E-mail: crodriguez@ias.csic.es
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Abstract

Triticum urartu, the A-genome donor of tetraploid and hexaploid wheats, is a potential source of novel alleles for crop improvement. A fertile amphiploid between T. urartu (2n = 2x = 14; AuAu) and durum wheat cv ‘Yavaros’ (Triticum turgidum ssp. durum; 2n = 4x = 28, AABB) was obtained as a first step to making the genetic variability of the wild ancestor available to durum wheat breeding. The amphiploid was backcrossed with ‘Yavaros’ and the offspring from this cross was selfed. A plant from this progeny (founder line) with 28 chromosomes and active x and y subunits of the Glu-A1 locus of T. urartu was selfed, which resulted in the obtaining of 98 pre-introgression lines (pre-ILs). In this work, a set of 78 wheat chromosome-specific microsatellite markers (simple sequence repeats, SSR), uniformly distributed over the A genome, was used for marker-assisted selection of T. urartu in a durum wheat background. A total of 57 SSRs allowed a clear discrimination between T. urartu and ‘Yavaros’. This set of markers was further used for characterizing the pre-ILs, identifying and defining the T. urartu introgressed regions. The applicability of these markers is discussed.

Keywords

introgressions linesmarker assisted selectionsimple sequence repeatsTriticum urartu
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 3 , August 2011 , pp. 439 - 444
Copyright
Copyright © NIAB 2011

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