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Characterization of high anthocyanin-producing tetraploid potato cultivars selected for breeding using morphological traits and microsatellite markers

Published online by Cambridge University Press:  06 November 2015

Roberto Tierno  and
Jose Ignacio Ruiz de Galarreta
Roberto Tierno
Affiliation:
NEIKER-Tecnalia, The Basque Institute for Agricultural Research and Development, PO Box 46, E-01080Vitoria, Spain
Jose Ignacio Ruiz de Galarreta*
Affiliation:
NEIKER-Tecnalia, The Basque Institute for Agricultural Research and Development, PO Box 46, E-01080Vitoria, Spain
*
*Corresponding author. E-mail: jiruiz@neiker.net
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Abstract

Purple- and red-fleshed potato cultivars constitute a great source of phenolic compounds, which may promote human health. Since the characterization of potato germplasm is a key step during the breeding process, the classification of high anthocyanin-producing tetraploid genotypes may facilitate the incorporation of phenolic-related traits in a potato breeding programme. A set of 18 high anthocyanin-producing underutilized tetraploid cultivars, which have been previously classified in terms of phytochemical content, have been characterized by both microsatellite markers (simple sequence repeat, SSR) and morphological descriptors. A wide genetic variability was found using 11 highly discriminatory SSR markers. The collection also displayed a large amount of variation for most morphological traits. The neighbour-joining trees defined by SSR markers and morphological descriptors revealed genetic and phenotypic relatedness of the potato genotypes. Despite the complexity of tetrasomic inheritance, high anthocyanin-producing tetraploid cultivars should be considered for potato breeding since they are adapted to long-day conditions and do not present undesirable characteristics that are found in native accessions or landraces.

Keywords

clusteringparental linephytochemicalsprincipal component analysisSolanum tuberosum LSSR
Type
Research Article
Information
Plant Genetic Resources , Volume 15 , Issue 2 , April 2017 , pp. 147 - 156
Copyright
Copyright © NIAB 2015 

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References

Ashkenazi, V, Chani, E, Lavi, U, Levy, D, Hillel, J and Veilleux, RE (2001) Development of microsatellite markers in potato and their use in phylogenetic and fingerprinting analyses. Genome 44: 50–62.Google Scholar
Barandalla, L, Ruiz de Galarreta, JI, Ríos, D and Ritter, E (2006) Molecular analysis of local potato cultivars from Tenerife Island using microsatellite markers. Euphytica 152: 283–291.Google Scholar
Bornet, B, Goraguer, G, Joly, G and Branchard, B (2002) Genetic diversity in European and Argentinian cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeats (ISSRs). Genome 45: 481–484.CrossRefGoogle ScholarPubMed
Brown, CR (2005) Antioxidants in potato. American Journal of Potato Research 82: 163–182.Google Scholar
Cadima, X, Veramendi, S and Gabriel, J (2013) Uso de marcadores moleculares microsatélite para el análisis de la diversidad genética de papa nativa de Bolivia. Journal of the Selva Andina Research Society 4: 18–30.Google Scholar
Côté, MJ, Leduc, L and Reid, A (2013) Evaluation of simple sequence repeat (SSR) markers established in Europe as a method for the identification of potato varieties grown in Canada. American Journal of Potato Research 90: 340–350.Google Scholar
Dice, LR (1945) Measures of the amount of ecologic association between species. Ecology 26: 297–302.Google Scholar
European Cultivated Potato Database (2015) Available at http://www.europotato.org/ (last retrieved 18 August 2015)..Google Scholar
Gavrilenko, T, Antonova, O, Ovchinnikova, A, Novikova, L, Krylova, E, Mironenko, N, Pendinen, G, Islamshina, A, Shvachko, N, Kiru, S, Kostina, L, Afanasenko, O and Spooner, D (2010) A microsatellite and morphological assessment of the Russian National cultivated potato collection. Genetic Resources and Crop Evolution 57: 1151–1164.Google Scholar
Ghislain, M, Zhang, D, Fajardo, D, Huamán, Z and Hijmans, RJ (1999) Marker-assisted sampling of the cultivated Andean potato Solanum phureja collection using RAPD markers. Genetic Resources and Crop Evolution 46: 547–555.Google Scholar
Ghislain, M, Spooner, DM, Rodríguez, F, Villamón, F, Núñez, J, Vásquez, C, Waugh, R and Bonierbale, M (2004) Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato. Theoretical and Applied Genetics 108: 881–890.Google Scholar
Görg, R, Schachtschabel, U, Ritter, E, Salamini, F and Gebhardt, C (1992) Discrimination among 136 tetraploid potato varieties by fingerprints using highly polymorphic DNA markers. Crop Science 32: 815–819.Google Scholar
Hamouz, K, Lachman, J, Hejtmánková, K, Pazderů, K, Čížek, M and Dvořák, P (2010) Effect of natural and growing conditions on the content of phenolics in potatoes with different flesh colour. Plant, Soil and Environment 56: 368–374.CrossRefGoogle Scholar
Hardigan, MA, Bamberg, J, Buell, CR and Douches, DS (2015) Taxonomy and genetic differentiation among wild and cultivated germplasm of Solanum sct. Petota . Plant Genome 8: 1–16.Google Scholar
Hejtmánková, K, Pivec, V, Trnková, E, Hamouz, K and Lachman, J (2009) Quality of coloured varieties of potatoes. Czech Journal of Food Science 27: S310–S313.Google Scholar
Jarque, CM and Bera, AK (1987) A test for normality of observations and regression residuals. International Statistical Review 55: 163–182.Google Scholar
Kawchuk, L, Lynch, D, Thomas, J, Penner, B, Sillito, D and Kulcsar, F (1996) Characterization of Solanum tuberosum simple sequence repeats and application to potato cultivar identification. American Potato Journal 73: 325–335.Google Scholar
Kim, JH, Joung, H, Kim, HY and Lim, YP (1998) Estimation of genetic variation and relationship in potato (Solanum tuberosum L.) cultivars using AFLP markers. American Journal of Potato Research 75: 107–112.Google Scholar
Lachman, J, Hamouz, K, Orák, M, Pivec, V and Dvorak, P (2008) Differences in phenolic content and antioxidant activity in yellow and purple fleshed potatoes grown in the Czech Republic. Plant, soil and Environment 54: 1–6.Google Scholar
Larkin, PJ and Scowcroft, WR (1981) Somaclonal variation, a novel source of variability from cell cultures for plant improvement. Theoretical and Applied Genetics 60: 197–214.Google Scholar
Lindhout, P, Meijer, D, Schotte, T, Hutten, RCB, Visser, RGF and van Eck, HJ (2011) Towards F1 hybrid seed potato breeding. Potato Research 54: 301–312.Google Scholar
Mantel, NA (1967) The detection of disease clustering and generalized regression approach. Cancer Research 27: 209–220.Google Scholar
Milbourne, D, Meyer, R, Bradshaw, JE, Baird, E, Bonar, N, Provan, J, Powell, W and Waugh, R (1997) Comparison of PCR-based marker system for the analysis of genetic relationships in cultivated potato. Molecular Breeding 3: 127–136.Google Scholar
Nei, M (1973) Analyses of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences 70: 3321–3323.Google Scholar
Nei, M and Saitou, N (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425.Google Scholar
Nováková, A, Šimáčková, K, Bárta, J and Čurn, V (2010) Utilization of DNA markers based on microsatellite polymorphism for identification of potato varieties cultivated in the Czech Republic. Journal of Central European Agriculture 4: 415–422.Google Scholar
Perrier, X and Jacquemoud-Collet, JP (2006) DARwin software. Available at http://darwin.cirad.fr/.Google Scholar
Powell, W, Machray, GC and Provan, J (1996) Polymorphism revealed by simple sequence repeats. Trends in Plant Science 1: 215–222.Google Scholar
Reid, A, Hof, L, Esselink, D and Vosman, B (2009) Potato cultivar genome analysis. In: Burns, R (ed.) Methods in Molecular Biology, Plant Pathology. vol. 508 Chapter 23. New York: Humana Press, pp. 295–308.Google Scholar
Reid, A, Hof, L, Felix, G, Ruecker, B, Tams, S, Milczynska, E, Esselink, D, Uenk, G, Vosman, B and Weitz, A (2011) Construction of an integrated microsatellite and key morphological characteristic database of potato varieties on the EU Common Catalogue. Euphytica 182: 239–249.Google Scholar
Reyes, LF, Miller, JC and Cisneros-Zevallos, L (2005) Antioxidant capacity, anthocyanins and total phenolics in purple- and red-flesh potato (Solanum tuberosum L.) genotypes. American Journal of Potato Research 82: 271–277.Google Scholar
Rohlf, FJ (2001) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Setauket, NY: Version 2.1 Exeter Software.Google Scholar
Ruiz de Galarreta, JI, Barandalla, L, Lorenzo, R, González, J, Ríos, DJ and Ritter, E (2007) Microsatellite variation in potato landraces from the island of La Palma. Spanish Journal of Agricultural Research 5: 186–192.Google Scholar
Ruiz de Galarreta, JI, Barandalla, L, Ríos, DJ, López, R and Ritter, E (2011) Genetic relationships among local potato cultivars from Spain using SSR markers. Genetic Resources and Crop Evolution 58: 383–395.Google Scholar
SAS(2011) SAS/STAT 9.3. User'Guides Survey Datas Analysis. Cary, NC: SAS Institute Inc.Google Scholar
Spooner, DM, Núñez, J, Trujillo, G, Herrera, MR, Guzmán, F and Ghislain, M (2007) Extensive simple sequence repeat genotyping of potato landraces supports a major reevaluation of their gene pool structure and classification. Proceedings of the National Academy of Sciences 104: 19398–19403.Google Scholar
The Kenosha Potato Collection Catalogue(2015) Available at http://www.curzio.com/N/PotatoCatalog.htm (last retrieved 18 August 2015).Google Scholar
Tierno, R, Riga, P and Ruiz de Galarreta, JI (2014) Dietary minerals and bioactive compounds in a set of purple and red fleshed potato genitors for breeding. In: Goffart, JP, Rolot, JL, Demeulemeester, K and Goeminne, M (eds) Proceedings of the 19th Triennial Conference EAPR 2014: Tuber Quality. EAPR, Brussels (Belgium), vol. 281, p. 306.Google Scholar
UPOV(1984) Industrial property and plant breeders rights (background paper: SYMP/1984/4). In: Anon., (ed.) Industrial Patents and Plant Breeders’ Rights – Their Proper Fields and Possibilities for Their Demarcation [Records of a Symposium held on the Occasion of the 18th Session of the Council of UPOV, 18 October 1984] . Geneva, Switzerland: UPOV, pp. 73–95.Google Scholar
Veilleux, RE, Shen, LY and Paz, MM (1995) Analysis of the genetic composition of anther-derived potato by randomly amplified polymorphic DNA and simple sequence repeats. Genome 38: 1153–1162.Google Scholar
Visser, RGF, Bachem, CWB, Boer, JM, Bryan, GJ, Chakrabati, SK, Feingold, S, Gromadka, R, Ham, RCHJ, Huang, S, Jacobs, JME, Kuznetsov, B, Melo, PE, Milbourne, D, Orjeda, G, Sagredo, B and Tang, X (2009) Sequencing the potato genome: outline and first results to come from the elucidation of the sequence of the world's third most important food crop. American Journal of Potato Research 86: 418–429.Google Scholar