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Molecular characterization of the Glu-Ay gene from Triticum urartu for its potential use in quality wheat breeding

Published online by Cambridge University Press:  30 March 2011

M. V. Gutiérrez ,
C. Guzmán ,
L. M. Martín  and
J. B. Alvarez
M. V. Gutiérrez
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
C. Guzmán
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
L. M. Martín
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
J. B. Alvarez*
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
*
*Corresponding author. E-mail: jb.alvarez@uco.es
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Abstract

Triticum urartu Thum. ex Gandil. is a wild species identified as A-genome donor for polyploid wheats, which could be used as gene source for wheat breeding. The high-molecular weight glutenin subunits are endosperm storage proteins that are associated with bread-making quality. In T. urartu, these proteins are encoded by the Ax and Ay genes at the Glu-Au1 locus. The Ay gene of 17 Glu-Au1 allelic variants previously detected in this species has been analysed using PCR amplification and digestion of the PCR products with two endonucleases (DdeI and PstI). The combination of two restriction patterns has revealed variations between the active and inactive alleles, and within each type. This variation, especially that detected among the active alleles, could enlarge the high-quality genetic pool of modern wheat and be used for bread-making quality improvement in durum and common wheat.

Keywords

electrophoresisgenetic resourcesgluteninquality breedingwild wheat
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 334 - 337
Copyright
Copyright © NIAB 2011

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