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Heteronuclear three-dimensional NMR spectroscopy of isotopically labelled biological macromolecules

Published online by Cambridge University Press:  17 March 2009

Stephen W. Fesik  and
Erik R. P. Zuiderweg
Stephen W. Fesik*
Affiliation:
Pharmaceutical Discovery Division, Abbott Laboratories, D-47G, AP9, Abbott Park, North Chicago, IL 60064, USA
Erik R. P. Zuiderweg
Affiliation:
Pharmaceutical Discovery Division, Abbott Laboratories, D-47G, AP9, Abbott Park, North Chicago, IL 60064, USA
*
*Author to whom correspondence should be addressed.
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Extract

Due to the development of two-dimensional Fourier transformation techniques (for reviews see Bax, 1982; Ernst et al. 1987), NMR spectroscopy has become a powerful tool for determining the 3D structures of small proteins (MW ≤ 10 kDa); for reviews see Wüthrich, 1986; Clore & Gronenborn, 1987. For larger molecules, however, the amount of detailed structural information that can be obtained using homonuclear 2D NMR techniques is limited because of the vast number of overlapping signals. In order to extend the capabilities of NMR to the study of larger systems, new approaches are required.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 23 , Issue 2 , May 1990 , pp. 97 - 131
Copyright
Copyright © Cambridge University Press 1990

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References

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