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Characterization of a novel barley β-amylase gene expressed only during early grain development

Published online by Cambridge University Press:  22 February 2007

Woosuk Jung ,
Ronald W. Skadsen  and
David M. Peterson
Woosuk Jung*
Affiliation:
Department of Agronomy, University of Wisconsin-Madison, Madison, WI 53706;
Ronald W. Skadsen
Affiliation:
Cereal Crops Research Unit, USDA-ARS, 501 Walnut St., Madison, WI 53705, USA
David M. Peterson
Affiliation:
Department of Agronomy, University of Wisconsin-Madison, Madison, WI 53706; Cereal Crops Research Unit, USDA-ARS, 501 Walnut St., Madison, WI 53705, USA
*
*Correspondence Fax.: 301-504-5728 Email: jungw@ba.ars.usda.gov
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Abstract

A developmentally regulated, novel β-amylase gene (ESDbamy) from Hordeum vulgare L. has 76% amino acid similarity with a previously described barley β-amylase. However, the 3' -end sequences of these β-amylases were different. The glycine-rich repeats, which are signature sequences of endosperm-type β-amylases, are absent in this novel ESDbamy gene. ESDbamy is highly expressed in the caryopsis during early development; however, it is not expressed in other tissues. Early seed development-specific (ESD) β-amylase mRNA levels and enzyme activities were higher in a shrunken endosperm mutant, seg8, than in the wild type. Seg8 has defective endosperm development, reduced starch synthesis and elevated monosaccharide concentrations

Keywords

β-amylasebarley (Hordeum vulgare L.)grain developmentshrunken endosperm mutant
Type
Research Article
Information
Seed Science Research , Volume 11 , Issue 4 , December 2001 , pp. 325 - 333
Copyright
Copyright © Cambridge University Press 2001

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