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Effects of Trifluralin Metabolites on Goosegrass (Eleusine indica) Root Meristems

Published online by Cambridge University Press:  12 June 2017

Kevin C. Vaughn  and
William C. Koskinen
Kevin C. Vaughn
Affiliation:
South. Weed Sci. Lab., USDA-ARS, P.O. Box 225, Stoneville, MS 38776
William C. Koskinen
Affiliation:
South. Weed Sci. Lab., USDA-ARS, P.O. Box 225, Stoneville, MS 38776
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Abstract

The effects of treatment with twelve metabolites of trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl) benzenamine] on the gross morphology and ultrastructure of trifluralin-susceptible (S) and -resistant (R) biotypes of goosegrass [Eleusine indica (L.) Gaertner. # ELEIN] root meristems were examined. Unlike trifluralin, ten of the twelve metabolites including the major metabolites of trifluralin caused no root tip swelling even at water-saturated concentrations. Two of the minor metabolites, 3-methoxy-2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl)benzenamine (TR-36M) and N-(2,6-dinitro-4-(trifluoromethyl)phenyl)-N-propylpropanamide (TR-40), caused root swelling when applied as a saturated solution. The most effective metabolite, TR-36M, caused root swelling at 10 μM or greater concentrations. Because trifluralin caused swelling of the root tips even at a 0.01 μM concentration, trifluralin is 1000-fold more effective than the most herbicidally active metabolite. Both herbicidally active metabolites are also much less effective than trifluralin at increasing the mitotic index. Structural studies indicate that the two herbicidally active metabolites arrest mitosis at prometaphase due to the loss of spindle microtubules, similar to trifluralin. Cortical microtubules are also affected by the highest concentrations of these two metabolites. A resistant biotype, in which mitosis is unaffected by trifluralin, was unaffected by any of the trifluralin metabolites.

Keywords

Dinitroaniline herbicidesherbicide resistanceELEIN
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 35 , Issue 1 , January 1987 , pp. 36 - 44
Copyright
Copyright © 1987 by the Weed Science Society of America 

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References

Literature Cited

1. Ashton, F. M. and Crafts, A. S. 1981. Dinitroanilines. Pages 201–223 in Mode of Action of Herbicides. John Wiley and Sons, New York.Google Scholar
2. Golab, T., Althaus, W. A., and Wooten, H. L. 1979. Fate of [14C]-trifluralin in soil. J. Agric. Food Chem. 27:163–179.Google Scholar
3. Hacskaylo, T. and Amato, V. A. 1968. Effect of trifluralin on roots of corn and cotton. Weed Sci. 16:513–515.Google Scholar
4. Hansch, C., Leo, A., Unger, S. H., Kim, K. H., Nikaitani, D., and Lier, E. J. 1973. “Aromatic” substituent constants for structure-activity correlations. J. Med. Chem. 16:1207–1216.Google Scholar
5. Hess, D. and Bayer, D. E. 1974. The effect of trifluralin on the ultrastructure of dividing cells of the root meristem of cotton (Gossypium hirsutum) L. 'Acala 4-42′). J. Cell Sci. 15:429–441.Google Scholar
6. Hess, D. and Bayer, D. E. 1977. Binding of the herbicide trifluralin to Chlamydamonas flagellar tubulin. J. Cell Sci. 24:351–360.CrossRefGoogle Scholar
7. Koskinen, W. L., Oliver, J. E., Kearney, P. C., and McWhorter, C. G. 1984. Effect of trifluralin soil metabolites on cotton growth and yield. J. Agric. Food Chem. 32:1246–1248.Google Scholar
8. Mudge, L. C., Gossett, B. T., and Murphy, T. R. 1984. Resistance of goosegrass (Eleusine indica) to dinitroaniline herbicides. Weed Sci. 32:591–594.Google Scholar
9. Murray, D. S., Santelmann, P. W., and Greer, H.A.L. 1973. Differential phytotoxicity of several dinitroaniline herbicides. Agron. J. 65:34–36.Google Scholar
10. Probst, G. W., Golab, T., Herberg, R. J., Holzer, F. T., Parka, S. T., Van Der Schans, C., and Tepe, J. B. 1967. Fate of trifluralin in soils and plants. J. Agric. Food Chem. 15:592–599.Google Scholar
11. Vaughn, K. C. 1986. Dinitroaniline resistance to goosegrass [Eleusine indica (L.) Gaertnr.] is due to an altered tubulin. Abstr. Weed Sci. Soc. Am. Page 77.Google Scholar
12. Vaughn, K. C. 1986. Cytological studies of dinitroaniline-resistant Eleusine . Pestic. Biochem. Physiol. 26:66–74.Google Scholar