Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-27T10:21:45.685Z Has data issue: false hasContentIssue false
  • English
  • Français

Variation in Pathogenicity and Host Range of Bipolaris sp. Causing Leaf Blight Disease on the Invasive Grass Microstegium vimineum

Published online by Cambridge University Press:  20 January 2017

N. M. Kleczewski ,
S. L. Flory  and
K. Clay
N. M. Kleczewski*
Affiliation:
Department of Biology, Indiana University, 1001 East 3rd Street, Bloomington, IN 47405
S. L. Flory
Affiliation:
Department of Biology, Indiana University, 1001 East 3rd Street, Bloomington, IN 47405
K. Clay
Affiliation:
Department of Biology, Indiana University, 1001 East 3rd Street, Bloomington, IN 47405
*
Corresponding author's E-mail: nkleczew@purdue.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

Microstegium vimineum is a widespread invasive grass that poses significant threats to forests and disturbed areas throughout the United States. Often, the large-scale, rapid spread of Microstegium prohibits management by traditional methods. Control of Microstegium may be possible through the use of a pathogen (referred to here as Bipolaris Mv) that causes leaf blight on Microstegium. Members of the fungal genus Bipolaris are known pathogens of many plants, including important agronomic crops. However, little is known about the biology and host range of Bipolaris Mv. We used a series of growth chamber and light bank experiments to determine the variation in Bipolaris Mv from different geographic origins and its ability to cause foliar lesions and chlorosis on Microstegium. We used petri plate and soil infestation assays to determine the effects of Bipolaris Mv on Microstegium emergence from seed, biomass, and root necrosis. Finally, we tested the host range of these fungi on economically and ecologically important plant species. All isolates increased disease on Microstegium foliage relative to controls, although the effects varied among isolates. Isolates increased root necrosis by 97% in petri plate assays and by 4% in soil infestation trials compared to controls. Infestation of soils with Bipolaris Mv reduced emergence of Microstegium from seed by 31% compared to controls, but did not affect root or stand biomass. Bipolaris Mv produced lesions on a range of grasses, including corn, sorghum, rye, and wheat, although lesion size varied with isolate. These results indicate that Bipolaris Mv may be an effective pathogen on Microstegium, but its use as a bioherbicide may be impractical because of its effects on a wide range of grasses.

Keywords

Microstegium (Mary's grass, stiltgrass), Microstegium vimineum (Trin.) A. Camusrimini rye, Secale cereale Lsorghum, Sorghum bicolor (L.) Moench ssp. bicolorwheat, Triticum aestivum Lcorn, Zea mays LBiocontrolfungiinvasive grasspathogenreservoir hosts
Type
Weed Management
Information
Weed Science , Volume 60 , Issue 3 , September 2012 , pp. 486 - 493
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Al-Sadi, A. M. and Deadman, M. L. 2010. Influence of seed-borne Cochliobolus sativus (Anamorph Bipolaris sorokiniana) on crown rot and root rot of barley and wheat. J. Phytopathol. 158:683–690.Google Scholar
Arabi, M. I. E. and Jawhar, M. 2007. Molecular and pathogenic variation identified among isolates of Cochliobolus sativus . Austral. Plant Pathol. 36:17–21.Google Scholar
Bashyal, B. M., Chand, R., Prasad, L. C., and Joski, A. K. 2011. Partial resistance components for the management of spot blotch pathogen Bipolaris sorokiniana of barley (Hordeum vulgare L.). Acta Phytopathol. Entomol. Hung. 46:49–57.Google Scholar
Bauer, J. T. and Flory, S. L. 2011. Suppression of the woodland herb Senna hebecarpa by the invasive grass Microstegium vimineum . Am. Midl. Nat. 165:105–115.CrossRefGoogle Scholar
Berbee, M. L., Pirseyedi, M., and Hubbard, S. 1999. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia. 91:964–977.Google Scholar
Bournival, B. L., Ginoza, H. S., Schenck, S., and Moore, P. H. 1994. Characterization of sugarcane response to Bipoalris sacchari: Inoculations and host-specific HC-Toxin. Phytopathology. 84:672–676.Google Scholar
Carvalho, M. P., Rodrigues, F. A., Silveira, P. R., Andrade, C. C. L., Baroni, J. C. P., Paye, H. S., and Loureiro, J. E. 2010. Rice resistance to brown spot mediated by nitrogen and potassium. J. Phytopathol. 158:160–166.Google Scholar
Chang, S. W. and Hwang, B. K. 2003. Effects of plant age, leaf position, inoculum density, and wetness period on Bipolaris coicis infection in adlays of differing resistance. Plant Dis. 87:821–826.Google Scholar
Civitello, D. J., Flory, S. L., and Clay, K. 2008. Exotic grass invasion reduces survival of Amblyomma americanum and Dermacentor variabilis ticks (Acari:Ixodidae). J. Med. Entomol. 45:867–872.Google Scholar
de Lima Nechet, K., Barreto, R. W., and Mizubuti, E. S. G. 2006. Bipolaris euphorbiae as a biological control agent for wild poinsettia (Euphorbia heterophylla): host-specificity and variability in pathogen and host populations. Biocontrol (Dordrecht) 51:259–275.Google Scholar
Dehne, H. W. and Oerke, E. C. 1985. Investigations on the occurrence of Cochliobolus sativus on barley and wheat 2. Infestion, colonization, and damage of stems and leaves. Z. Pflanzenk. Pflanzens. (J. Plant Dis. Prot.) 92:606–617.Google Scholar
DeMeester, J. E. and Richter, D. D. B. 2010. Differences in wetland nitrogen cycling between the invasive grass Microstegium vimineum and a diverse plant community. Ecol. Appl. 20:609–619.Google Scholar
Den Breeyen, A. and Charudattan, R. 2009. biological control of invasive weeds in forests and natural areas by using microbial agents. Pages 189–209 in Inderjit, ed. Management of Invasive Weeds. New York Springer.Google Scholar
Dodd, J. L. 1993. Recent developments in the maize pathogen Bipolaris zeicola (Shoemaker). Maydica. 38:201–204.Google Scholar
Domiciano, G. P., Rodrigues, F. A., Vale, F. X. R., Xavier, M. S., Moreira, W. R., Andrade, C. C. L., and Pereira, S. C. 2010. Wheat resistance to spot blotch potentiated by silicon. J. Phytopathol. 158:334–343.Google Scholar
Duczek, L. J., Jones-Flory, L. L., Reed, S. L., Bailey, K. L., and Lafond, G. P. 1996. Sporulation of Bipolaris sorokiniana on the crowns of crop plants grown in Saskatchewan. Can. J. Plant Sci. 76:861–867.CrossRefGoogle Scholar
Egel, D. S., Harikrishnan, R., , R. D. and Martyn, R. 2005. First report of Fusarium oxysporum f. sp niveum race 2 as causal agent of Fusarium wilt of watermelon in Indiana. Plant Dis. 89:108–108.CrossRefGoogle ScholarPubMed
Ehrenfeld, J. G., Kourtev, P., and Huang, W. Z. 2001. Changes in soil functions following invasions of exotic understory plants in deciduous forests. Ecol. Appl. 11:1287–1300.Google Scholar
Farr, D. F. and Rossman, A. Y. 2011. Fungal Databases, Systemic Mycology and Microbiology Laborotory, ARS-USDA. http://nt.ars-grin.gov/fungaldatabases/fungaldatabases/ Acessed: October 24, 2011.Google Scholar
Flory, S. L. 2010. Management of Microstegium vimineum invasions and recovery of resident plant communities. Restor. Ecol. 18:103–112.Google Scholar
Flory, S. L. and Clay, K. 2010a. Non-native grass invasion alters native plant composition in experimental communities. Biol. Invasions. 12:1285–1294.CrossRefGoogle Scholar
Flory, S. L. and Clay, K. 2010b. Non-native grass invasion suppresses forest succession. Oecologia. 164:1029–1038.CrossRefGoogle ScholarPubMed
Flory, S. L., Kleczewski, N. M., and Clay, K. 2011. Ecological consequences of pathogen accumulation on an invasive grass. Ecosphere. 2(10):120.Google Scholar
Fradkin, A. and Patrick, Z. A. 1985. Effect of mathric potential, pH, temperature, and clay minerals on bacterial colonization of conidia of Cochliobolus sativus and their survival in soil. Can. J. Plant Pathol.–Rev. Can. Phytopathol. 7:19–27.Google Scholar
Ghazvini, H. and Tekauz, A. 2007. Virulence diversity in the population of Bipolaris sorokiniana . Plant Dis. 91:814–821.Google Scholar
Ghazvini, H. and Tekauz, A. 2008. Host–pathogen interactions among barley genotypes and Bipolaris sorokiniana isolates. Plant Dis. 92:225–233.Google Scholar
Hallett, S. G. 2005. Where are the bioherbicides? Weed Sci. 53:404–415.Google Scholar
Heil, M. 2010. Plastic defence expression in plants. Evol. Ecol. 24:555–569.Google Scholar
Hodges, C. F. and Watschke, G. A. 1975. Pathogenicity of soil-borne Bipolairs sorokiniana on seed and roots of 3 perennial grasses. Phytopathology. 65:398–400.Google Scholar
Horsfall, J. G. and Barratt, R. W. 1945. An improved grading system for measuring plant diseases. Phytopathology. 35:655.Google Scholar
Jawhar, M. and Arabi, M. I. E. 2009. Heterogeneity in the internal transcribed spacers of the ribosomal DNA in Cochliobolus sativus as revealed by IRAP. J. Plant Pathol. 91:123–127.Google Scholar
Kaur, S., Singh, K., Satija, D. R., and Singh, P. 2004. Quantitative estimates of host response in wheat genotypes against leaf blight pathogen. Crop Improv. 31:48–56.Google Scholar
Kleczewski, N. M. and Flory, S. L. 2010. Leaf blight disease on the invasive grass Microstegium vimineum caused by a Bipolaris sp. Plant Dis. 94:807–811.Google Scholar
Knight, T. M., Dunn, J. L., Smith, L. A., Davis, J., and Kalisz, S. 2009. Deer facilitate invasive plant success in a Pennsylvania forest understory. Nat. Areas J. 29:110–116.CrossRefGoogle Scholar
Kumar, J., Schafer, P., Huckelhoven, R., Langen, G., Baltruschat, H., Stein, E., Nagarajan, S., and Kogel, K. H. 2002. Bipolaris sorokiniana, a cereal pathogen of global concern: cytological and molecular approaches towards better control. Mol. Plant Pathol. 3:185–195.Google Scholar
Lakshmanan, P., Jeyarajan, R., and Vidhyasekaran, P. 1990. Leaf and stem blight of Euphorbia geniculata incited by Bipolaris zeicola . Phytoparasitica. 18:353–355.Google Scholar
Liljeroth, E., Franzon-Almgren, I., and Gunnarsson, T. 1996. Root colonization by Bipolaris sorokiniana in different cereals and relations to lesion development and natural root cortical cell death. J. Phytopathol.–Phytopathol. Z. 144:301–307.Google Scholar
Liljeroth, E., Jansson, H. B., and Schafer, W. 1993. Transformation of Bipolaris sorokiniana with the GUS gene and use for studying fungal colonization of barley roots. Phytopathology. 83:1484–1489.Google Scholar
Martinez, A. D., Franzener, G., and Stangarlin, J. R. 2010. Damages caused by Bipolaris maydis in Panicum maximum cv. Tanzania. Semin. Cienc. Agrar. 31:863–870.Google Scholar
Mirabolfathy, M. and Ershad, D. 2006. Bipolaris, Curvularia, Drechslera and Exserohilum diseases of turfgrass in Iran. Iran. J. Plant Pathol. 42:83–85.Google Scholar
Nelson, R. R. 1960. Evolution of sexuality and pathogenicity 1. Interspecific crosses in the genus Helminthosporium . Phytopathology. 50:375–377.Google Scholar
Nelson, R. R. and Kline, D. M. 1964. Evolution of sexuality and pathogenicity IV. Effects of geographic origin and host association on the pathogenicity of isolates of Helminthosporium with similar conidial morphology. Phytopathology. 54:1207–1209.Google Scholar
Nyvall, R. F. and Hu, A. 1997. Laboratory evaluation of indigenous North American fungi for biological control of purple loosestrife. Biol. Control. 8:37–42.Google Scholar
Scardaci, S. C. and Webster, R. K. 1981. Antagonism between the cereal root rot pathogens Fusarium graminearum and Bipolaris sorokiniana . Plant Dis. 65:965–967.Google Scholar
Simao, M. C. M., Flory, S. L., and Rudgers, J. A. 2010. Experimental plant invasion reduces arthropod abundance and richness across multiple trophic levels. Oikos. 119:1553–1562.Google Scholar
Smiley, R. W., Backhouse, D., Lucas, P., and Paulitz, T. C. 2009. Diseases which Challenge global wheat production—root, crown, and culm rots. Pages 125–153 in Carter, B. F., ed. Wheat: Science and Trade. Ames, IA Blackwell Publishing.Google Scholar
Stack, R. W. 1992. Bipolaris . Pages 94–99 in Singleton, L. L., Mihail, J. D., and Rush, C. M., eds. Methods for Research on Soilborne Pathogenic Fungi. St. Paul, MN APS Press.Google Scholar
Strickland, M. S., Callaham, M. A., Davies, C. A., Lauber, C. L., Ramirez, K., Richter, D. D., Fierer, N., and Bradford, M. A. 2010. Rates of in situ carbon mineralization in relation to land-use, microbial community and edaphic characteristics. Soil Biol. Biochem. 42:260–269.Google Scholar
Traut, E. J. and Warren, H. L. 1993. Expansion of lesions induced by races 1, 2, and 3 of Bipolaris zeicola . Maydica. 38:215–221.Google Scholar
Trevathan, L. E. 1992. Seedling emergence, plant height, and root mass of tall fescue grown in soil infested with Cochliobolus sativus . Plant Dis. 76:270–273.Google Scholar
Trevathan, L. E. 1996. Performance of endophyte-free and endophyte-infected tall fescue seedlings in soil infested with Cochliobolus sativus . Can. J. Plant Pathol.–Rev. Can. Phytopathol. 18:415–418.Google Scholar
Tscharntke, T., Bommarco, R., Clough, Y., Crist, T. O., Kleijn, D., Rand, T. A., Tylianakis, J. M., van Nouhuys, S., and Vidal, S. 2007. Conservation biological control and enemy diversity on a landscape scale. Biol. Control. 43:294–309.CrossRefGoogle Scholar
Upadhyay, M. K., Jain, D., Tiwari, K., Singh, A. and Verma, H. N. 2011. Exploitation of Fungi: A potential approach for the management of weeds. Proc. Nat. Acad. Sci. India Sect. B–Biol. Sci. 81:69–75.Google Scholar
[USDA-NRCS] U.S. Department of Agriculture–Natural Resources Conservation Service. 2011. The PLANTS Database. http://plants.usda.gov. Accessed: December 22, 2011. Greensboro, NC: National Plant Data Team.Google Scholar
Winder, R. S. and Vandyke, C. G. 1990. The pathogenicity, virulence, and biocontrol potential of 2 Bipolaris species on johnsongrass (Sorghum haplepense). Weed Sci. 38:89–94.Google Scholar
Yandoc, C. B. and Charudattan, R. 2004. Suppression of cogongrass (Imperata cylindrica) by a bioherbicidal fungus and plant competition. Weed Sci. 52:649–653.Google Scholar