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Comparisons Between X-ray Film- and Phosphorescence Imaging-Based Autoradiography for the Visualization of Herbicide Translocation

Published online by Cambridge University Press:  20 January 2017

Glenn Wehtje ,
Michael E. Miller ,
Timothy L. Grey  and
William R. Brawner Jr
Glenn Wehtje*
Affiliation:
Agronomy and Soils, Auburn University, Auburn, AL 36849
Michael E. Miller
Affiliation:
Department of Biological Sciences, Auburn University, Auburn, AL 36849
Timothy L. Grey
Affiliation:
Crop and Soil Science, University of Georgia, Tifton, GA 31794
William R. Brawner Jr
Affiliation:
Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL 36849
*
Corresponding author's E-mail: wehtjgr@auburn.edu
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Abstract

Autoradiography is a radioisotope-based technique that allows absorbed and translocated herbicide to be visualized. Autoradiographs are traditionally produced with X-ray film and exposure times of several weeks. Phosphorescence imaging (PI) was investigated as an alternative autoradiography procedure. Smallflower morningglory plants were root-exposed to a series of 14C-atrazine concentrations, producing a series of increasing foliar radioactivity concentrations (i.e., dosage) that ranged from marginal to excessive with respect to autoradiography. Autoradiographs were subsequently produced from these 14C-atrazine-dosed plants using both the X-ray film and the PI technique. Autoradiographs from both techniques were of excellent quality and nearly identical when the dosage was ∼20 to 70 Bq/mg. However, PI produces an acceptable image in dosages either above or below this optimum range. A 1-d exposure time was sufficient with PI, and longer exposure times were not detrimental to image quality. In contrast, a 3-wk exposure time was required with X-ray film. Autoradiographs of selected herbicides are presented to further demonstrate the utility of PI.

Keywords

Atrazinesmallflower morningglory, Jacquemontia tamnifolia (L.) Griseb IAQTADigital imagingradioisotopes
Type
Teaching/Education
Information
Weed Technology , Volume 21 , Issue 4 , December 2007 , pp. 1109 - 1114
Copyright
Copyright © Weed Science Society of America 

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