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Acetolactate Synthase (ALS) Inhibitor-Resistant Wild Buckwheat (Polygonum convolvulus) in Alberta

Published online by Cambridge University Press:  20 January 2017

Hugh J. Beckie ,
Suzanne I. Warwick  and
Connie A. Sauder
Hugh J. Beckie*
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2
Suzanne I. Warwick
Affiliation:
AAFC, Eastern Cereal and Oilseed Research Centre, K.W. Neatby Building, Central Experimental Farm, Ottawa, Ontario, Canada K1A 0C6
Connie A. Sauder
Affiliation:
AAFC, Eastern Cereal and Oilseed Research Centre, K.W. Neatby Building, Central Experimental Farm, Ottawa, Ontario, Canada K1A 0C6
*
Corresponding author's E-mail: hugh.beckie@agr.gc.ca
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Abstract

Wild buckwheat is the most abundant broadleaf weed across the Prairie region of western Canada. Acetolactate synthase (ALS)-inhibiting herbicides are commonly used to control this species and other broadleaf weeds in cereal crops. A field survey in Alberta in 2007 identified a single population that was putatively resistant to ALS-inhibiting herbicides. In herbicide resistance screening in the greenhouse, all F1 progeny tested were resistant to the ALS-inhibiting herbicides thifensulfuron/tribenuron, a sulfonylurea herbicide, or florasulam, a triazolopyrimidine herbicide; dose response of shoot biomass indicated the population was 10- and 20-fold less sensitive to thifensulfuron/tribenuron and florasulam, respectively, than a susceptible control population. ALS gene sequencing of 24 F1 progeny indicated that the Trp574Leu target-site mutation was responsible for conferring ALS-inhibitor resistance in this biotype, the first global report of ALS-inhibitor resistance for this species. Because this mutation typically endows high-level resistance across all five ALS-inhibitor classes, this wild buckwheat biotype may only be controlled by a different site-of-action herbicide.

Polygonum convolvulus es la maleza de hoja ancha más abundante a lo largo de la región Pradera del occidente de Canadá. Los herbicidas inhibidores de la Acetolactate synthase (ALS) son comúnmente usados para controlar esta especie y otras malezas de hoja ancha en el cultivo de cereales. Una investigación de campo realizada en Alberta en 2007, identificó una sola población que era putativamente resistente a los herbicidas inhibidores ALS. En la evaluación de resistencia a los herbicidas en el invernadero, toda la progenie F1 evaluada fue resistente a los herbicidas inhibidores ALS thifensulfuron/tribenuron, un herbicida sulfonylurea, o florasulam, un herbicida triazolopyrimidine. La respuesta a dosis de la biomasa de la parte aérea de las plantas, indicó que la población fue 10 y 20 veces menos sensible a thifensulfuron/tribenuron y florasulam, respectivamente, que una población susceptible testigo. La secuencia genética ALS de 24 descendientes F1 indicó que la mutación del sitio de acción -Trp574Leu fue responsable de conferir la resistencia al inhibidor ALS para este biotipo, siendo este el primer reporte global de resistencia al inhibidor ALS para esta especie. Ya que esta mutación, típicamente confiere un nivel alto de resistencia entre todas las cinco clases de inhibidores ALS, este biotipo de P. convolvulus podría ser controlado únicamente por un herbicida con un sitio de acción diferente.

Keywords

Florasulamthifensulfurontribenuronwild buckwheat, Polygonum convolvulus L. POLCOALS gene sequencingALS-inhibitor resistanceherbicide resistancetarget-site mutation
Type
Notes
Information
Weed Technology , Volume 26 , Issue 1 , March 2012 , pp. 156 - 160
Copyright
Copyright © Weed Science Society of America 

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