Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-10T16:30:02.037Z Has data issue: false hasContentIssue false
  • English
  • Français

Acetolactate Synthase Inhibitor–Resistant False Cleavers (Galium spurium) in Western Canada

Published online by Cambridge University Press:  20 January 2017

Hugh J. Beckie ,
Suzanne I. Warwick ,
Connie A. Sauder ,
Gina M. Kelln  and
Chris Lozinski
Hugh J. Beckie*
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada
Suzanne I. Warwick
Affiliation:
AAFC, Eastern Cereal and Oilseed Research Centre, K.W. Neatby Building, Central Experimental Farm, Ottawa, Ontario K1A 0C6, Canada
Connie A. Sauder
Affiliation:
AAFC, Eastern Cereal and Oilseed Research Centre, K.W. Neatby Building, Central Experimental Farm, Ottawa, Ontario K1A 0C6, Canada
Gina M. Kelln
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada
Chris Lozinski
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada
*
Corresponding author's E-mail: hugh.beckie@agr.gc.ca
Get access Rights & Permissions [Opens in a new window]

Abstract

Cleavers species (false cleavers and catchweed bedstraw) are among the top 10 most abundant weeds across the prairie region of western Canada, and are increasing in relative abundance at the fastest rate since the 1970s. In 2008, two false cleavers populations from Tisdale and Choiceland, Saskatchewan, were suspected of acetolactate synthase (ALS) –inhibitor resistance. Dose-response experiments were conducted with the use of imazethapyr and florasulam, both ALS inhibitors, as well as fluroxypyr, a synthetic auxin. Additionally, a 1,954–base-pair region of the ALS gene including sites known to confer ALS resistance were sequenced. Both populations were highly resistant to imazethapyr (resistance factors greater than 100), one population (Tisdale) was highly resistant to florasulam (Choiceland population susceptible, although a second, larger screening of 200 individuals indicated low frequency [2%] florasulam resistance), and both populations were susceptible to fluroxypyr. All sequenced Tisdale individuals screened with imazethapyr posessed the Trp574Leu mutation. In contrast, three point mutations were found for Choiceland individuals sequenced: Ser653Asn, Trp574Leu, and Asp376Glu. These ALS target-site mutations have not been documented previously in this species.

Las especies Galium spurium y Galium aparine están entre las diez malezas más abundantes a lo largo de la región Pradera del occidente de Canadá y están incrementando en abundancia relativa a la tasa más rápida desde los años setenta. En 2008, dos poblaciones de G. spurium de Tisdale y Choiceland, Saskatchewan, fueron sospechosas de ser resistentes a los inhibidores de acetolactate synthase (ALS). Se realizaron experimentos de respuesta a dosis usando imazethapyr y florasulam, ambos inhibidores de ALS, así como también fluroxypyr, una auxina sintética. Adicionalmente, fue secuenciada una región 1954-pb del gen ALS que incluye sitios que se sabe confieren resistencia ALS. Ambas poblaciones fueron altamente resistentes a imazethapyr (factores de resistencia mayores que 100); una población (Tisdale) fue altamente resistente a florasulam. La población Choiceland resultó ser susceptible, aunque una segunda y mayor selección de 200 individuos indicó baja frecuencia de resistencia a florasulam (2%), y ambas poblaciones fueron susceptibles a fluroxypyr. Todos los individuos secuenciados de Tisdale, tratados con imazethapyr, exhibieron la mutación Trp574Leu. En contraste, tres puntos de mutación se encontraron en los individuos secuenciados de Choiceland: Ser653Asn, Trp574Leu y Asp376Glu. Estas mutaciones ALS en el sitio-objetivo no habían sido documentadas previamente en estas especies.

Keywords

Florasulamfluroxypyrimazethapyrcatchweed bedstraw, Galium aparine L.false cleavers, Galium spurium L. GALSPALS gene sequencingALS-inhibitor resistanceherbicide resistancetarget-site mutation
Type
Notes
Information
Weed Technology , Volume 26 , Issue 1 , March 2012 , pp. 151 - 155
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Beckie, H. J., Lozinski, C., and Shirriff, S. 2009. Alberta Weed Survey of Herbicide-Resistant Weeds in 2007. Weed Survey Series Publication 09-1. Saskatoon, Canada Agriculture and Agri-Food Canada. 36 p.Google Scholar
Beckie, H. J. and Tardif, F. J. 2011. Herbicide cross resistance in weeds. Crop Prot. In press.Google Scholar
Gomez, K. A. and Gomez, A. A. 1984. Statistical Procedures for Agricultural Research (2nd ed.). New York, NY Wiley. 680 p.Google Scholar
Hall, L. M., Stromme, K. M., Horsman, G. P., and Devine, M. D. 1998. Resistance to acetolactate synthase inhibitors and quinclorac in a biotype of false cleavers (Galium spurium). Weed Sci. 46:390–396.Google Scholar
Horsman, G. P. and Devine, M. D. 2000. Molecular basis of ALS resistance in a biotype of false cleavers (Galium spurium L.). Weed Sci. Soc. Am. Abstr. 40:5.Google Scholar
Leeson, J. Y. and Neeser, C. 2010. A preview of residual weed population shifts in Alberta—1970s to 2010. Proc. 2010 National Mtg. Pinawa, Canada Canadian Weed Science Society, http://www.weedscience.ca. Accessed: September 2010.Google Scholar
Leeson, J. Y., Thomas, A. G., Hall, L. M., Brenzil, C. A., Andrews, T., Brown, K. R., and Van Acker, R. C. 2005. Prairie Weed Surveys of Cereal, Oilseed and Pulse Crops from the 1970s to the 2000s. Weed Survey Series Publication 05-1. Saskatoon, Canada Agriculture and Agri-Food Canada. 395 p.Google Scholar
Malik, N. and Vanden Born, W. H. 1987. False cleavers competition and control in rapeseed. Can. J. Plant Sci. 67:839–844.CrossRefGoogle Scholar
Malik, N. and Vanden Born, W. H. 1988. The biology of Canadian weeds. 86. Galium aparine L. and Galium spurium L. Can. J. Plant Sci. 68:481–499.Google Scholar
Rozen, S. and Skaletsky, H. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132:365–386.Google Scholar
SAS. 1999. SAS/STAT User's Guide. Version 8. Cary, NC Statistical Analysis Systems Institute. 1243 p.Google Scholar
Saskatchewan Ministry of Agriculture. 2011. Guide to Crop Protection: Weeds, Plant Diseases, Insects. Regina, Canada Saskatchewan Ministry of Agriculture. 468 p. http://www.agriculture.gov.sk.ca/Guide_to_Crop_Protection. Accessed: April, 2011.Google Scholar
Sathasivan, K., Haughn, G. W., and Murai, N. 1990. Nucleotide sequence of a mutant acetolactate synthase gene from imidazolinone resistant Arabidopsis thaliana var. Columbia. Nucleic Acids Res. 18:2188.Google Scholar
Steel, G. D. and Torrie, J. H. 1980. Principles and Procedures of Statistics: A Biometrical Approach. 2nd ed. New York, NY McGraw-Hill. 633 p.Google Scholar
Sun, J., Wang, J. X., Zhang, H. J., Liu, J. L., Huan, Z. B., and Jin, T. 2011. Study on mutations in ALS for resistance to tribenuron-methyl in Galium aparine L. Agric. Sci. China 10(1):86–91 (Sci. Agric. Sin. 2010-05).Google Scholar
Yoshimura, Y., Beckie, H. J., and Matsuo, K. 2006. Transgenic oilseed rape along transportation routes and port of Vancouver in western Canada. Environ. Biosafety Res. 5:67–75.CrossRefGoogle ScholarPubMed