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Evaluation of Epicuticular Wax Removal from Whole Leaves with Chloroform

Published online by Cambridge University Press:  12 June 2017

Thomas A. Bewick ,
Donn G. Shilling  and
Robert Querns
Thomas A. Bewick
Affiliation:
Hortic. Sci. Dep., Univ. Fla., Gainesville, 32611
Donn G. Shilling
Affiliation:
Agron. Dep., Univ. Fla., Gainesville, 32611
Robert Querns
Affiliation:
Agron. Dep., Univ. Fla., Gainesville, 32611
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Abstract

Leaves of torpedograss and American black nightshade were extracted with chloroform at room temperature. A 2-s dip was sufficient to remove most of the epicuticular wax from torpedograss. However, epicuticular hydrocarbon weight represented only 6.4% of the total extract weight and 6.94 μg g−1 fresh weight of chlorophyll were found in the 2-s extract. This represented 25% of the chlorophyll detected in the 232-h extract. In American black nightshade, epicuticular hydrocarbons continued to be removed from the leaf surface up to 6 h of extraction. Epicuticular hydrocarbons represented 0.6% of total extract weight. In the 6-h extract, 4.02 μg g−1 fresh weight of chlorophyll were found. This represented 17% of the chlorophyll detected in the 232-h extract. Evaluation of leaf surfaces using scanning electron microscopy indicated that epicuticular wax was being removed from torpedograss leaves up to 1 h. However, there was little visible evidence for wax extraction from the surface of American black nightshade leaves.

Keywords

American black nightshade, Solanum americanum Mill., # SOLAMtorpedograss, Panicum repens L., # PANRECuticle removalsolvent removalPanicum repensSolanum americanumPANRESOLAM
Type
Research
Information
Weed Technology , Volume 7 , Issue 3 , September 1993 , pp. 706 - 716
Copyright
Copyright © 1993 by the Weed Science Society of America 

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