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Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes

Published online by Cambridge University Press:  15 February 2019

A.A.A. Silva ,
M.N.P. Silva ,
L.B.F. Figueiredo ,
J.D. Gonçalves ,
M.J.S. Silva ,
M.L.G. Loiola ,
B.D.M. Bastos ,
R.A. Oliveira ,
L.G.M. Ribeiro  and
R.S Barberino
...Show all authors
A.A.A. Silva*
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
M.N.P. Silva
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
L.B.F. Figueiredo
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
J.D. Gonçalves
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
M.J.S. Silva
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
M.L.G. Loiola
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
B.D.M. Bastos
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
R.A. Oliveira
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
L.G.M. Ribeiro
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
R.S Barberino
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, Brazil
B.B. Gouveia
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, Brazil
A.P.O. Monte
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, Brazil
D.M. Nogueira
Affiliation:
Brazilian Agricultural Research Corporation, Embrapa Tropical Semiarid, Brazil
M.F. Cordeiro
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
M.H.T. Matos
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, Brazil
E.S. Lopes Júnior
Affiliation:
Laboratory of Physiology and Biotechnology of Animal Reproduction, Federal University of San Francisco Valley, Petrolina, Brazil
*
Address for correspondence: E.S. Lopes Júnior. Universidade Federal do Vale do São Francisco (UNIVASF). Colegiado de Medicina Veterinária - Laboratório de Fisiologia e Biotecnologia da Reprodução Animal. Rodovia BR 407 Km 12, Lote 543, Projeto de Irrigação Nilo Coelho, Petrolina, PE 56300–990, Brasil. Tel: +558721014816. Fax: +558721014845. E-mail: edilson.lopes@univasf.edu.br
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Summary

The present study aimed to investigate the effect of quercetin as an alternative antioxidant to cysteamine on in vitro maturation. Oocytes were collected from goat ovaries, destined for in vitro maturation and distributed into three groups: CIS group, oocytes were immersed in MIV base medium; in Groups Q4 and Q8, oocytes were immersed in the medium of the CIS group, adding 4 μM or 8 μM of quercetin, respectively, and cultured for 24 h at 38.5°C with 5% CO2. The CIS and Q4 groups presented the same percentage of expanded cumulus cells, but the per cent in the Q8 group was significantly lower than that of the other groups (P<0.05). The oocyte retraction rate in the Q8 group was higher (P<0.05) than in the CIS and Q4 groups. Treatment with 8 μM of quercetin presented a lower proportion of expanded oocytes than the CIS group and 4 μM of quercetin (P<0.05). The percentage of MII oocytes was higher in the Q4 group than in the CIS group (P<0.05), but the percentages in the CIS and Q8 groups were similar. The rate of apoptosis was higher in the CIS group than in the other groups (P<0.05). In addition, oocytes matured with 4 μM quercetin showed higher mitochondrial activity than matured oocytes in the CIS and Q8 groups (P<0.05). In conclusion, 4 μM of quercetin can be used as an alternative to cysteamine in the in vitro maturation of goat oocytes.

Keywords

AntioxidantFlavonoidGSHOvary
Type
Research Article
Information
Zygote , Volume 26 , Issue 6 , December 2018 , pp. 465 - 470
Copyright
© Cambridge University Press 2019 

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