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Ultrastructural changes in the follicular epithelium of Ceratophrys cranwelli previtellogenic oocytes

Published online by Cambridge University Press:  01 August 2007

E.I. Villecco ,
M.E. Mónaco  and
S.S. Sánchez
E.I. Villecco
Affiliation:
Departamento de Biología del Desarrollo, Instituto Superior de Investigaciones Biológicas (INSIBIO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) y Universidad Nacional de Tucumán (UNT), Chacabuco 461, 4000, San Miguel de Tucumán, Tucumán, Argentina
M.E. Mónaco
Affiliation:
Departamento de Biología del Desarrollo, Instituto Superior de Investigaciones Biológicas (INSIBIO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) y Universidad Nacional de Tucumán (UNT), Chacabuco 461, 4000, San Miguel de Tucumán, Tucumán, Argentina
S.S. Sánchez*
Affiliation:
Departamento de Biología del Desarrollo, Instituto Superior de Investigaciones Biológicas (INSIBIO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) y Universidad Nacional de Tucumán (UNT), Chacabuco 461, 4000, San Miguel de Tucumán, Tucumán, Argentina
*
All correspondence to: S.S. Sánchez, Departamento de Biología del Desarrollo, INSIBIO (CONICET-UNT), Chacabuco 461, 4000-San Miguel de Tucumán, Argentina. Tel: +54 81 4107214. Fax: +54 81 4247752 ext. 7004. e-mail: ssanchez@fbqf.unt.edu.ar
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Summary

In this work we carried out ultrastructural, autoradiographic and biochemical analyses of the follicular epithelium during C. cranwelli previtellogenesis. This study revealed that the follicular epithelium in early previtellogenesis is constituted of a single layer of squamous homogeneous cells. During mid-previtellogenesis two types of cells develop: dark cells and clear cells. The follicular dark cells are actively involved in the synthesis of RNA, which is transferred to the oocyte through the interface. In late previtellogenesis the dark cells show apoptotic characteristics such as chromatin condensation, DNA fragmentation and cytoplasm shrinkage. This process forms apoptotic bodies that seem to be engulfed by the oocyte. Our results show evidence that, during mid- and late C. cranwelli previtellogenesis, the follicular epithelium undergoes remodelling processes interacting with the oocyte.

Keywords

AmphibianApoptosisFollicular cellsPrevitellogenic oocyte
Type
Research Article
Information
Zygote , Volume 15 , Issue 3 , August 2007 , pp. 273 - 283
Copyright
Copyright © Cambridge University Press 2007

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