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Bovine somatic cell nuclear transfer using mitomycin C-mediated chemical oocyte enucleation

Published online by Cambridge University Press:  30 April 2019

M.T. Moura Open the ORCID record for M.T. Moura [Opens in a new window] ,
R.V. Sousa ,
C.M. Lucci  and
R. Rumpf
M.T. Moura*
Affiliation:
Embrapa Recursos Genéticos e Biotecnologia, 70770–917, Brasília-DF, Brazil Universidade de Brasília, 70910–900, Brasília-DF, Brazil
R.V. Sousa
Affiliation:
Embrapa Recursos Genéticos e Biotecnologia, 70770–917, Brasília-DF, Brazil
C.M. Lucci
Affiliation:
Universidade de Brasília, 70910–900, Brasília-DF, Brazil
R. Rumpf
Affiliation:
Embrapa Recursos Genéticos e Biotecnologia, 70770–917, Brasília-DF, Brazil Universidade de Brasília, 70910–900, Brasília-DF, Brazil
*
Address for correspondence: Marcelo Tigre Moura. Federal University of São Paulo-UNIFESP, Diadema-SP, Brazil. Tel: +55 11 4044 0500 extension 3474. E-mail: marcelotmoura@gmail.com
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Summary

Chemical oocyte enucleation holds the potential to ease somatic cell nuclear transfer (SCNT), although high enucleation rates remain limited to micromanipulation-based approaches. Therefore, this study aimed to test mitomycin C (MMC) for use in bovine functional chemical oocyte enucleation. Incubation of denuded eggs in 10 µg ml−1 MMC for different periods did not affect most maturation rates (0.5 h: 85.78%A, 1.0 h: 72.77%B, 1.5 h: 83.87%A, and 2.0 h: 82.05%A) in comparison with non-treated controls (CTL; 85.77%A). Parthenogenetic development arrest by MMC was efficient at cleavage (CTL: 72.93%A, 0.5 h: 64.92%A,B, 1.0 h: 60.39%B,C, 1.5 h: 66.35%A,B, and 2.0 h: 53.84%C) and blastocyst stages (CTL: 33.94%A, 0.5 h: 7.58%B, 1.0 h: 2.47%C, 1.5 h: 0.46%C, and 2.0 h: 0.51%C). Blastocysts were obtained after nuclear transfer (NT) using MMC enucleation [NT(MMC): 4.54%B] but at lower rates than for the SCNT control [NT(CTL): 26.31%A]. The removal of the meiotic spindle after MMC incubation fully restored SCNT blastocyst development [NT(MMC+SR): 24.74%A]. Early pregnancies were obtained by the transfer of NT(MMC) and NT(MMC+SR) blastocysts to synchronized recipients. In conclusion, MMC leads to functional chemical oocyte enucleation during SCNT and further suggests its potential for application towards technical improvements.

Keywords

CattleCytoplastDNA damageLivestockNuclear transplantation
Type
Research Article
Information
Zygote , Volume 27 , Issue 3 , June 2019 , pp. 137 - 142
Copyright
© Cambridge University Press 2019 

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Footnotes

†

Current address: Federal University of São Paulo-UNIFESP, 09972–270, Diadema-SP, Brazil.

‡

Current address: Geneal Genética e Biotecnologia Animal, 38020-970, Uberaba-MG, Brazil.

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