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Chromosomes of mouse primary spermatocytes undergo meiotic divisions after incorporation into homologous immature oocytes

Published online by Cambridge University Press:  26 September 2008

Atsuo Ogura ,
Teruhiko Wakayama ,
Osamu Suzuki ,
Tae-Young Shin ,
Junichiro Matsuda  and
Yoshiro Kobayashi
Atsuo Ogura*
Affiliation:
Department of Veterinary Science, National Institute of Health, Tokyo, Japan
Teruhiko Wakayama
Affiliation:
Department of Veterinary Science, National Institute of Health, Tokyo, Japan
Osamu Suzuki
Affiliation:
Department of Veterinary Science, National Institute of Health, Tokyo, Japan
Tae-Young Shin
Affiliation:
Department of Veterinary Science, National Institute of Health, Tokyo, Japan
Junichiro Matsuda
Affiliation:
Department of Veterinary Science, National Institute of Health, Tokyo, Japan
Yoshiro Kobayashi
Affiliation:
Department of Veterinary Science, National Institute of Health, Tokyo, Japan
*
Atsuo Ogura, Department of Veterinary Science, National Institute of Health, 1–23–1, Toyama, Shinjuku, Tokyo 162, Japan. Fax: +81-3-5285-1150. e-mail: aogxura@nih.go.jp.
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Summary

The primary spermatocytes used were male germ cells at prophase I. The present study was undertaken to see whether bivalent chromosomes of mouse primary spermatocytes can undergo meiotic divisions within maturing oocytes and participate in subsequent embryonic development. Primary spermatocytes (pachytene to diplotene) freshly collected from the testes of mature males were electrofused with immature oocytes shortly before or after germinal vesicle breakdown. After culture in MEM-α medium for 15 h, most (> 90%) of the oocytes containing spermatocyte chromosomes underwent maturation and arrested at metaphase II (Mil). Among 23 Mil oocytes examined, 17 (74%) had one group of chromosomes and one polar body, indicating that male chromosomes had intermingled with those of the females and completed the first meiotic division. Chromosome analyses of these Mil oocytes demonstrated their diploidy. The metaphase chromosomes were transferred to enucleated Mil oocytes freshly recovered from superovulated mice. After artificial activation, the reconstructed Mil oocytes resumed meiosis and developed to the morula/blastocyst stage. However, no pups were born following embryo transfer into recipient females. These findings indicate that the chromosomes of primary spermatocytes undergo meiotic divisions in maturing oocytes and participate in the formation of diploid embryos.

Keywords

ElectrofusionMeiosisMouseSpermatocyte
Type
Article
Information
Zygote , Volume 5 , Issue 2 , May 1997 , pp. 177 - 182
Copyright
Copyright © Cambridge University Press 1997

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