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The effects of magnetic separation on cryopreserved bovine spermatozoa motility, viability and cryo-capacitation status

Published online by Cambridge University Press:  14 December 2012

S.S.M. Faezah ,
F.M.Y Zuraina ,
J.H.F. Farah ,
O. Khairul ,
N.I. Hilwani ,
M.I. Iswadi ,
C.N. Fang ,
I. Zawawi ,
O.M. Abas  and
S.I. Fatimah
S.S.M. Faezah
Affiliation:
Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor D.E., Malaysia.
F.M.Y Zuraina
Affiliation:
Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor D.E., Malaysia.
J.H.F. Farah
Affiliation:
Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
O. Khairul
Affiliation:
Forensic Science Programme, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
N.I. Hilwani
Affiliation:
Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor D.E., Malaysia.
M.I. Iswadi
Affiliation:
Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
C.N. Fang
Affiliation:
Department of Biomedical Sciences, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
I. Zawawi
Affiliation:
Institut Bioteknologi Veterinar Kebangsaan, Bukit Dinding, 27000 Jerantut, Pahang, Malaysia.
O.M. Abas
Affiliation:
Agro-Biotechnology Institute, Malaysia, d/o MARDI Headquarters, 43400 Serdang, Selangor D.E., Malaysia.
S.I. Fatimah*
Affiliation:
Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
*
All correspondence to S.I. Fatimah. Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. Tel: +603 9289 7641. Fax: +603 2693 9687. E-mail: timi@medic.ukm.my
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Summary

Cryopreservation is a technique used to preserve cells for long-time storage. It is widely used in agriculture to store male gametes in liquid nitrogen. The aim of this study was to determine the optimum thawing temperature and time for samples subjected to annexin V magnetic-activated cell sorting (AnMACS) as the sperm preparation technique. Pooled semen samples from three ejaculates were divided into two groups. The treatment group was subjected both to AnMACS and to being cryopreserved, whilst the control group was cryopreserved directly without MACS. Post-thaw analysis was carried out for samples thawed at either 20°C for 13 s, 37°C for 30 s, 40°C for 7 s, 60°C for 6 s or 80°C for 5 s. Sperm kinematics, viability and capacitation status were determined for samples subjected to all thawing temperatures described. Results showed that thawing at 37°C for 13 s for MACS-processed samples was a superior option compared with other thawing procedures; there was a significant difference in P < 0.05 values for curvilinear velocity (VCL μm/s) and sperm straightness (STR %) when samples were thawed at 40°C for 7 s, with fewer capacitated spermatozoa (P < 0.05) when samples were thawed at 37°C for 30 s, 40°C for 7 s or 60°C for 6 s. Hence, we can speculate that the use of AnMACS as the sperm preparation technique can somehow enhance sperm cryosurvival rate after cryopreservation, however the fertilization potential of these cells has yet to be determined.

Keywords

CapacitationMagnetic-activated cell sorting systemThawingViability
Type
Research Article
Information
Zygote , Volume 22 , Issue 3 , August 2014 , pp. 378 - 386
Copyright
Copyright © Cambridge University Press 2012 

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References

Aamdal, J. & Andersen, K. (2007). Fast thawing of semen frozen in straws. Reprod. Dom. Anim. 3, 22–4.Google Scholar
Aitken, R.J. (2011). The capacitation-apoptosis highway: oxysterols and mammalian sperm function. Biol. Reprod. 85, 9–12.CrossRefGoogle ScholarPubMed
Anel, L., Gomes-Alves, S., Alvarez, M., Borragan, S., Anel, E., Nicolas, M., Martinez-Pastor, F. & de Paz, P. (2010). Effect of basic factors of extender composition on post-thawing quality of brown bear electroejaculated spermatozoa. Theriogenology 74, 643–51.CrossRefGoogle ScholarPubMed
Bailey, J., Bilodeau, J. & Cormier, N. (2000). Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. J. Androl. 21, 1–7.Google Scholar
Brouwers, J.F., Boerke, A., Silva, P.F.N., Garcia-Gil, N., van Gestel, R.A., Helms, J.B., van de Lest, C.H.A. & Gadella, B.M. (2011). Mass spectrometric detection of cholesterol oxidation in bovine sperm. Biol. Reprod. 85, 128–36.Google Scholar
Calamera, J., Buffone, M., Doncel, G., Brugo-Olmedo, S., de Vincentiis, S., Calamera, M., Storey, B. & Alvarez, J. (2010). Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa. Fertil. Steril. 93, 789–94.Google Scholar
Córdova-Izquierdo, A., Oliva, J., Lleó, B., Garcia-Artiga, C., Corcuera, B. & Pérez-Gutiérrez, J. (2006). Effect of different thawing temperatures on the viability, in vitro fertilizing capacity and chromatin condensation of frozen boar semen packaged in 5 ml straws. Anim. Reprod. Sci. 92, 145–54.Google Scholar
de Lamirande, E., Jiang, H., Zini, A., Kodama, H. & Gagnon, C. (1997). Reactive oxygen species and sperm physiology. Reproduction 2, 48–54.Google ScholarPubMed
Dirican, E. K., Özgün, O. D., Akarsu, S., Akın, K. O., Ercan, Ö., Uğurlu, M., Çamsarı, Ç., Kanyılmaz, O., Kaya, A. & Ünsal, A. (2008). Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction. J. Assoc. Reprod. Genet. 25, 375–81.Google Scholar
Donnelly, E., Lewis, S., McNally, J. & Thompson, W. (1998). In vitro fertilization and pregnancy rates: the influence of sperm motility and morphology on IVF outcome. Fertil. Steril. 70, 305–14.CrossRefGoogle ScholarPubMed
Grunewald, S., Paasch, U. & Glander, H. (2001). Enrichment of non-apoptotic human spermatozoa after cryopreservation by immunomagnetic cell sorting. Cell Tissue Banking 2, 127–33.CrossRefGoogle ScholarPubMed
Grunewald, S., Paasch, U., Said, T., Rasch, M., Agarwal, A. & Glander, H. (2006). Magnetic-activated cell sorting before cryopreservation preserves mitochondrial integrity in human spermatozoa. Cell Tissue Banking 7, 99–104.Google Scholar
Ibrahim, S.F., Osman, K., Das III, S., Othman, A.M., Majid, N.A. & Rahman IV, M.P.A. (2008). Basic Research Clinics 64, 545–50.Google Scholar
Ibrahim, S.F., Jaffar, F.H.F., Osman, K. & Syed, S.F. (2011). Bull spermatozoa motility: optimization of coenzyme q10 and alpha-lipoic acid concentration. IIOAB J. 2, 8–13.Google Scholar
Kagan, V.E., Fabisiak, J.P., Shvedova, A.A., Tyurina, Y.Y., Tyurin, V.A., Schor, N.F. & Kawai, K. (2000). Oxidative signaling pathway for externalization of plasma membrane phosphatidylserine during apoptosis. FEBS Letts 477, 1–7.Google Scholar
Kang, J.H. & Park, J.K. (2004). Cell separation technology. Yeast, 90.Google Scholar
Kim, S., Yu, D. & Kim, Y. (2009). Effects of cryopreservation on phosphatidylserine translocation, intracellular hydrogen peroxide, and DNA integrity in canine sperm. Theriogenology 73, 282–92.Google Scholar
Kurz, A., Viertel, D., Herrmann, A. & Müller, K. (2005). Localization of phosphatidylserine in boar sperm cell membranes during capacitation and acrosome reaction. Reproduction 130, 615–26.Google Scholar
Langlais, J. & Roberts, K.D. (1985). A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa. Gamete Res. 12, 183–224.CrossRefGoogle Scholar
Li, G., Saenz, J., Godke, R. & Devireddy, R. (2006). Effect of glycerol and cholesterol-loaded cyclodextrin on freezing-induced water loss in bovine spermatozoa. Reproduction 131, 875–86.Google Scholar
Martin, S.J., Finucane, D.M., Amarante-Mendes, G.P., O'Brien, G.A. & Green, D.R. (1996). Phosphatidylserine externalization during CD95-induced apoptosis of cells and cytoplasts requires ICE/CED-3 protease activity. J. Biol. Chem. 271, 28753–6.Google Scholar
Maxwell, W. & Johnson, L. (1997). Chlortetracycline analysis of boar spermatozoa after incubation, flow cytometric sorting, cooling, or cryopreservation. Mol. Reprod. Dev. 46, 408–18.Google Scholar
Mazur, P. & Schmidt, J. (1968). Interactions of cooling velocity, temperature, and warming velocity on the survival of frozen and thawed yeast. Cryobiology 5, 1–17.Google Scholar
Mortimer, S. & Mortimer, D. (1990). Kinematics of human spermatozoa incubated under capacitating conditions. J. Androl. 11, 195–203.Google Scholar
Muiño, R., Rivera, M., Rigau, T., Rodriguez-Gil, J. & Peña, A. (2008). Effect of different thawing rates on post-thaw sperm viability, kinematic parameters and motile sperm subpopulations structure of bull semen. Anim. Reprod. Sci. 109, 50–64.Google Scholar
Nur, Z., Dogan, I., Soylu, M. & Ak, K. (2003). Effect of different thawing procedures on the quality of bull semen. Revue de Médecine Vétérinaire 154, 487–90.Google Scholar
Pace, M., Sullivan, J., Elliott, F., Graham, E. & Coulter, G. (1981). Effects of thawing temperature, number of spermatozoa and spermatozoal quality on fertility of bovine spermatozoa packaged in .5-ml French straws. J. Anim. Sci. 53, 693–701.Google Scholar
Pickett, B., Berndtson, W. & Sullivan, J. (1978). Influence of seminal additives and packaging systems on fertility of frozen bovine spermatozoa. J. Anim. Sci. 47 (Suppl. 2), 12–47.Google Scholar
Robbins, R., Saacke, R. & Chandler, P. (1976). Influence of freeze rate, thaw rate and glycerol level on acrosomal retention and survival of bovine spermatozoa frozen in French straws. J. Anim. Sci. 42, 145–54.CrossRefGoogle ScholarPubMed
Rodriguez, P., Valdez, L., Zaobornyj, T., Boveris, A. & Beconi, M. (2011). Nitric oxide and superoxide anion production during heparin-induced capacitation in cryopreserved bovine spermatozoa. Reprod. Domest. Anim. 46, 74–81.Google Scholar
Ryan, L., O'Callaghan, Y.C. & O'Brien, N.M. (2005). The role of the mitochondria in apoptosis induced by 7β-hydroxycholesterol and cholesterol-5β,6β-epoxide. Br. J. Nutr. 94, 519–25.CrossRefGoogle ScholarPubMed
Said, T., Grunewald, S., Paasch, U., Rasch, M., Agarwal, A. & Glander, H. (2005). Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates. Fertil. Steril. 83, 1442–6.Google Scholar
Said, T., Agarwal, A., Zborowski, M., Grunewald, S., Glander, H. & Paasch, U. (2008). Utility of magnetic cell separation as a molecular sperm preparation technique. J. Androl. 29, 134–42.Google Scholar
Söderquist, L., Madrid-Bury, N. & Rodriguez-Martinez, H. (1997). Assessment of ram sperm membrane integrity following different thawing procedures. Theriogenology 48, 1115–25.Google Scholar
Vermes, I., Haanen, C., Steffens-Nakken, H. & Reutellingsperger, C. (1995). A novel assay for apoptosis flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled annexin V. J. Immunol. Methods 184, 39–51.CrossRefGoogle ScholarPubMed
Wang, W., Abeydeera, L., Fraser, L. & Niwa, K. (1995). Functional analysis using chlortetracycline fluorescence and in vitro fertilization of frozen–thawed ejaculated boar spermatozoa incubated in a protein-free chemically defined medium. Reproduction 104, 305–13.Google Scholar
Ward, C. & Storey, B. (1984). Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay. Dev. Biol. 104, 287–96.Google Scholar
Watson, P. (1995). Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod. Fertil. Dev. 7, 871–92.Google Scholar
Yanagimachi, R. (1994). Mammalian fertilization. In The Physiology of Reproduction, 2nd edn (eds Knobil, E. & Neill, J.D.) pp. 189–317. New York: Raven Press.Google Scholar