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Expression of steroidogenic enzymes and TGFβ superfamily members in follicular cells of prepubertal gilts with distinct endocrine profiles

Published online by Cambridge University Press:  10 May 2021

Veronica Hoyos-Marulanda ,
Cristina S. Haas ,
Karina L. Goularte ,
Monique T. Rovani ,
Rafael G. Mondadori ,
Arnaldo D. Vieira ,
Bernardo G. Gasperin  and
Thomaz Lucia Jr Open the ORCID record for Thomaz Lucia Jr [Opens in a new window]
Veronica Hoyos-Marulanda
Affiliation:
ReproPel, Universidade Federal de Pelotas, Pelotas-RS, Brazil Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas-RS, Brazil
Cristina S. Haas
Affiliation:
ReproPel, Universidade Federal de Pelotas, Pelotas-RS, Brazil Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brazil
Karina L. Goularte
Affiliation:
ReproPel, Universidade Federal de Pelotas, Pelotas-RS, Brazil Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brazil
Monique T. Rovani
Affiliation:
Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Rafael G. Mondadori
Affiliation:
ReproPel, Universidade Federal de Pelotas, Pelotas-RS, Brazil Instituto de Biologia, Universidade Federal de Pelotas, Pelotas-RS, Brazil
Arnaldo D. Vieira
Affiliation:
ReproPel, Universidade Federal de Pelotas, Pelotas-RS, Brazil Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brazil
Bernardo G. Gasperin
Affiliation:
ReproPel, Universidade Federal de Pelotas, Pelotas-RS, Brazil Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brazil
Thomaz Lucia Jr*
Affiliation:
ReproPel, Universidade Federal de Pelotas, Pelotas-RS, Brazil Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brazil
*
Author for correspondence: Thomaz Lucia Jr. ReproPel, Faculdade de Veterinária, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil. E-mails: thomaz.lucia@ufpel.edu.br, tluciajr@gmail.com
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Summary

Regulation of the transforming growth factor beta (TGFβ) superfamily by gonadotrophins in swine follicular cells is not fully understood. This study evaluated the expression of steroidogenic enzymes and members of the TGFβ superfamily in prepubertal gilts allocated to three treatments: 1200 IU eCG at D −3 (eCG); 1200 IU eCG at D −6 plus 500 IU hCG at D −3 (eCG + hCG); and the control, composed of untreated gilts. Blood samples and ovaries were collected at slaughter (D0) and follicular cells were recovered thereafter. Relative gene expression was determined by real-time PCR. Serum progesterone levels were greater in the eCG + hCG group compared with the other groups (P < 0.01). No differences were observed in the expression of BMP15, BMPR1A, BMPR2, FSHR, GDF9, LHCGR and TGFBR1 (P > 0.05). Gilts from the eCG group presented numerically greater mean expression of CYP11A1 mRNA than in the control group that approached statistical significance (P = 0.08) and greater expression of CYP19A1 than in both the eCG and the control groups (P < 0.05). Expression of BMPR1B was lower in the eCG + hCG treatment group compared with the control (P < 0.05). In conclusion, eCG treatment increased the relative expression of steroidogenic enzymes, whereas treatment with eCG + hCG increased serum progesterone levels. Although most of the evaluated TGFβ members were not regulated after gonadotrophin treatment, the downregulation of BMPR1B observed after treatment with eCG + hCG and suggests a role in luteinization regulation.

Keywords

BMPR1BFollicleGenesGiltsGonadotrophinSteroids
Type
Research Article
Information
Zygote , Volume 30 , Issue 1 , February 2022 , pp. 65 - 71
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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