Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-10T16:44:13.087Z Has data issue: false hasContentIssue false
  • English
  • Français

Histochemical and morphological features of biopsied and stripped oocytes from the Brazilian endangered teleost pirapitinga, Brycon nattereri (Characiformes)

Published online by Cambridge University Press:  15 January 2014

Alexandre N. Maria ,
Laura H. Orfão ,
Elizete Rizzo ,
Alexandre Ninhaus-Silveira  and
Ana T.M. Viveiros
Alexandre N. Maria
Affiliation:
Department of Animal Science (DZO), Federal University of Lavras, UFLA, Lavras, MG, Brazil.
Laura H. Orfão
Affiliation:
Department of Animal Science (DZO), Federal University of Lavras, UFLA, Lavras, MG, Brazil.
Elizete Rizzo
Affiliation:
Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, UFMG, Belo Horizonte, MG, Brazil.
Alexandre Ninhaus-Silveira
Affiliation:
Department of Biology and Animal Science, São Paulo State University - FEIS, Ilha Solteira, SP, Brazil.
Ana T.M. Viveiros*
Affiliation:
Department of Animal Science (DZO), Federal University of Lavras, UFLA, PO Box 3037, Lavras, MG, 37200–000, Brazil. Department of Animal Science (DZO), Federal University of Lavras, UFLA, Lavras, MG, Brazil.
*
All correspondence to: Ana TM Viveiros. Department of Animal Science (DZO), Federal University of Lavras, UFLA, PO Box 3037, Lavras, MG, 37200–000, Brazil. e-mail: ana.viveiros@dzo.ufla.br; anatmviveiros@hotmail.com
Get access Rights & Permissions [Opens in a new window]

Summary

The aim of the present study was to characterize biopsied and stripped oocytes from the Brazilian endangered teleost pirapitinga (Brycon nattereri) using histochemical and morphological analyses. Biopsied oocytes had a mean diameter of 2.225 mm (modal diameter: 2.312 mm), complete vitellogenesis and a central or slightly eccentric nucleus. Neutral polysaccharides were detected in the follicular cells, zona radiata and yolk globules, while acidic polysaccharides were detected in the follicular cells and cortical alveoli. Ten out of the 19 females treated with two doses of carp pituitary extract (cPE) released oocytes, which were also analysed. Stripping occurred 292 ± 39 degree-hours after the second dose of cPE and led to a mean spawning weight of 36.2 g, 10% spawning index, 241 oocytes/g of ova, 8222 oocytes/female and 23 oocytes/g of body weight. Stripped oocytes had a mean diameter of 2.33 mm and a mode at 2.375 mm, were weakly adhesive and coloration ranged from wine to brown. Under scanning electron microscopy, stripped oocytes exhibited a single funnel-shaped micropyle located at the animal pole and a zona radiata that measured 7.7 μm in thickness with eight pore canals/μm2. Oocyte morphology in Brycon nattereri is similar to that found in other species of the genus, except for the larger size and weaker adhesiveness. These findings provide essential information for a better understanding of the reproductive biology of B. nattereri and the establishment of conservation measures for this threatened species.

Keywords

FishOocyte surfaceReproductionSpawningUltrastructure
Type
Research Article
Information
Zygote , Volume 23 , Issue 3 , June 2015 , pp. 360 - 366
Copyright
Copyright © Cambridge University Press 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alexandre, J.S., Ninhaus-Silveira, A., Veríssimo-Silveira, R., Buzollo, H., Senhorini, J.A. & Chaguri, M.P. (2010). Structural analysis of the embryonic development in Brycon cephalus (Günther, 1869). Zygote 18, 173–83.Google Scholar
Andrade-Talmelli, E.F., Kavamoto, E.T., Narahara, M.Y. & Fenerich-Verani, N. (2002). Reprodução Induzida da piabanha, Brycon insignis (Steindachner, 1876) (Characiformes, Bryconinae), mantida em confinamento. Rev. Bras. Zootec. 31, 803–11.Google Scholar
Bazzoli, N. & Godinho, H.P. (1994). Cortical alveoli in oocytes of freshwater neotropical teleost fish. Boll. Zool. 61, 301–08.Google Scholar
Ganeco, L.N. & Nakaghi, L.S.O. (2003). Morfologia da superfície dos ovócitos e caracterização da micrópila de piracanjuba, Brycon orbignyanus, sob microscopia eletrônica de varredura. Acta Sci. 25, 227–31.Google Scholar
Ganeco, L.N., Franceschini-Vicentini, I.B. & Nakaghi, L.S.O. (2009). Structural analysis of fertilization in the fish Brycon orbignyanus . Zygote 17, 93–9.Google Scholar
Gomes, B.V.C., Scarpelli, R.S., Arantes, F.P., Sato, Y., Bazzoli, N. & Rizzo, E. (2007). Comparative oocyte morphology and early development in three species of trahiras from the São Francisco River basin, Brazil. J. Fish Biol. 70, 1412–29.Google Scholar
Gomiero, L.M. & Braga, F.M.S. (2007). Reproduction of pirapitinga do sul (Brycon opalinus, Cuvier, 1819) in the Parque Estadual da Serra do Mar-Núcleo Santa Virgínia, São Paulo, Brazil. Braz. J. Biol. 67, 541–49.Google Scholar
Grier, H. & Neidig, C. (2011). Gonads and gametes of fishes. In Cryopreservation in Aquatic Species, 2nd edn (eds. Tiersch, T.R. & Green, C.C.), pp. 19–32. Baton Rouge, LA, USA: The World Aquaculture Society.Google Scholar
Isaú, Z.A., Rizzo, E., Amaral, T.B., Mourad, N.M.N. & Viveiros, A.T.M. (2013). Structural analysis of oocytes, post-fertilization events and embryonic development of the Brazilian endangered teleost Brycon insignis (Characiformes). Zygote 21, 85–94.Google Scholar
Kramer, D.L. (1978). Terrestrial group spawning of Brycon petrosus in Panamá. Copeia 1978, 536–37.CrossRefGoogle Scholar
Lima, F.C.T., Albrecht, M.P., Pavanelli, C.S. & Vono, V. (2007). Threatened fishes of the world: Brycon nattereri Günther, 1864 (Characidae). Environ. Biol. Fishes 1, 1–2.Google Scholar
Narahara, M.Y., Andrade-Talmelli, E.F., Kavamoto, E.T. & Godinho, H.M. (2002). Reprodução induzida da pirapitinga-do-sul, Brycon opalinus (Curvier, 1819), mantida em condições de confinamento. R. Bras. Zootec. 31, 1070–75. [in Portugese]Google Scholar
Ohta, T., Iwamatsu, T., Tanaka, M. & Yashimoto, Y. (1990). Cortical alveolus breakdown in the eggs of the freshwater teleost Rhodeus ocellatus ocellatus . Anat. Rec. 227, 486–96.CrossRefGoogle ScholarPubMed
Oliveira, A.V., Viveiros, A.T.M., Maria, A.N., Freitas, R.T.F. & Izaú, Z.A. (2007). Sucesso do resfriamento e congelamento de sêmen de pirapitinga Brycon nattereri . Arq. Bras. Med. Vet. Zootec. 59, 1509–15. [in Portugese]Google Scholar
Pearse, A.G.E. (1985). Histochemistry: Theoretical and Applied. London: Churchill Livingstone.Google Scholar
Riehl, R. & Patzner, R.A. (1998). The modes of egg attachment in teleost fishes. Ital. J. Zool. 65, 415–20.Google Scholar
Rizzo, E. & Bazzoli, N. (1991). The zona pellucida of the Brazilian white piranha, Serrasalmus brandtii Reinhardt, 1874 (Pisces, Characidae): a cytological and cytochemical study. Funct. Dev. Morphol. 1, 21–4.Google Scholar
Rizzo, E. & Godinho, H.P. (2003). Superfície de ovos de peixes Characiformes e Siluriformes. In Águas, Peixes e Pescadores do São Francisco das Minas Gerais (eds. Godinho, H.P. & Godinho, A. L.), pp. 115–132. Belo Horizonte, MG, Brazil: PUC Minas. [in Portugese]Google Scholar
Rizzo, E., Sato, Y., Barreto, B.P. & Godinho, H.P. (2002). Adhesiveness and surface patterns of eggs in neotropical freshwater teleosts. J. Fish Biol. 61, 615–32.CrossRefGoogle Scholar
Rosa, R.S. & Lima, F.C.T. (2008). Os peixes brasileiros ameaçados de extinção. In Livro Vermelho da Fauna Brasileira Ameaçada de Extinção, 2nd vol. (eds. Machado, A.B.M., Drummond, G.M. & Paglia, A.P.), pp. 8–285. Belo Horizonte, MG, Brazil: Fundação Biodiversitas. [in Portugese]Google Scholar
Sato, Y., Fenerich-Verani, N. & Godinho, H.P. (2003). Reprodução induzida de peixes da bacia do São Francisco. In Águas, Peixes e Pescadores do São Francisco das Minas Gerais (eds. Godinho, H.P. & Godinho, A.L.), pp. 275–89. Belo Horizonte, MG, Brazil: PUC Minas. [in Portugese]Google Scholar
Tyler, C.R. & Sumpter, J.P. (1996). Oocyte growth and development in teleosts. Rev. Fish Biol. Fish. 6, 287–318.CrossRefGoogle Scholar
Van Zutphen, L.F., Baumans, V. & Beynen, A.C. (2001). Principles of Laboratory Animal Science: A Contribution to the Humane Use and Care of Animals and to the Quality of Experimental Results. Revised edition. Amsterdam: Elsevier.Google Scholar
Viveiros, A.T.M., Maria, A.N., Amaral, T.B., Orfão, L.H., Isaú, Z.A. & Verissimo-Silveira, R. (2012). Spermatozoon ultrastructure and sperm cryopreservation of the Brazilian dry season spawner fish pirapitinga, Brycon nattereri . Aquac. Res. 43, 546–55.Google Scholar
Weber, A.A., Arantes, F.P., Sato, Y., Rizzo, E. & Bazzoli, N. (2012). Oocyte adhesiveness and embryonic development of Astyanax bimaculatus (Linnaeus, 1758) (Pisces: Characidae). Zygote 21,198–202.CrossRefGoogle ScholarPubMed
West, G. (1990). Methods of assessing ovarian development in fishes: a review. Aust. J. Mar. Freshwater Res. 41, 199–222.Google Scholar
Zaniboni-Filho, E., Reynalte-Tataje, D. & Weingartner, M. (2006). Potencialidad del género Brycon en la piscicultura brasileña. Rev. Colomb. Cienc. Pecu. 19, 233–40. [in Spanish]Google Scholar