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Non-plasmalemmal localisation of the major ganglioside in sea urchin eggs

Published online by Cambridge University Press:  26 September 2008

Hidehiko Shogomori ,
Kazuyoshi Chiba ,
Hideo Kubo  and
Motonori Hoshi
Hidehiko Shogomori
Affiliation:
Tokyo Institute of Technology, Yokohama and Tokyo Metropolitian Insitute of Medical Science, Tokyo, Japan
Kazuyoshi Chiba
Affiliation:
Tokyo Institute of Technology, Yokohama and Tokyo Metropolitian Insitute of Medical Science, Tokyo, Japan
Hideo Kubo
Affiliation:
Tokyo Institute of Technology, Yokohama and Tokyo Metropolitian Insitute of Medical Science, Tokyo, Japan
Motonori Hoshi*
Affiliation:
Tokyo Institute of Technology, Yokohama and Tokyo Metropolitian Insitute of Medical Science, Tokyo, Japan
*
Motonori Hoshi, Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 227, Japan. Fax: 81-45-923-0368.
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Summary

M5 ganglioside (NeuGcα2–6Glcβl-' Cer) is the predominant glycosphingolipid in sea urchin eggs. Distribution of M5 ganglioside was studied in unfertilised and fertilised eggs of the sea urchin Hemicentrotus pulcherrimus by indirect immunofluorescence microscopy. In the cortices of unfertilised eggs, anti-M5 antibody strongly stained the submembranous, polygonal and tubular network of endoplasmic reticulum that was revealed by a membrane-staining dye, DiIC18(3). In addition to the cortical network of endoplasmic reticulum, at least two morphologically distinct vesicles were positive to the antibody. In the cortices isolated from fertilised eggs 30 min after insemination, the antibody stained only a similar network of endoplasmic reticulum, presumably the one reconstructed 5–10 min after fertilisation. During mitosis the endoplasmic reticulum is known to aggregate within the asters of the mitotic apparatus. Indeed, the antibody stained the asters and (more strongly) the vesicular components attaching to the periphery of the mitotic apparatus.

Keywords

Anti-ganglioside antibodyEndoplasmic reticulumGangliosideMitotic apparatusSeaurchin egg
Type
Review Article
Information
Zygote , Volume 1 , Issue 3 , August 1993 , pp. 215 - 223
Copyright
Copyright © Cambridge University Press 1993

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