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Paternal breed effects on expression of IGF-II, BAK1 and BCL2-L1 in bovine preimplantation embryos

Published online by Cambridge University Press:  02 September 2014

Mehdi Vafaye Valleh ,
Mojtaba Tahmoorespur ,
Morteza Daliri Joupari ,
Hesam Dehghani ,
Mikkel Aabech Rasmussen ,
Poul Hyttel  and
Lotte Strøbech
Mehdi Vafaye Valleh*
Affiliation:
Department of Animal Science, Faculty of Agriculture, University of Zabol, P.O. Box 98615–538, Zabol, Iran.
Mojtaba Tahmoorespur
Affiliation:
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, I.R. Iran.
Morteza Daliri Joupari
Affiliation:
National Institutes for Genetic Engineering and Biotechnology, Karaj, Iran.
Hesam Dehghani
Affiliation:
Embryonic and Stem Cell Biology and Biotechnology Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Mikkel Aabech Rasmussen
Affiliation:
Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Groennegardsvej, 7,1870 Frederiksberg C, Denmark.
Poul Hyttel
Affiliation:
Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Groennegardsvej, 7,1870 Frederiksberg C, Denmark.
Lotte Strøbech
Affiliation:
Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Groennegardsvej, 7,1870 Frederiksberg C, Denmark.
*
All correspondence to: Mehdi Vafaye Valleh. Department of Animal Science, Faculty of Agriculture, University of Zabol, P.O. Box 98615-538, Zabol, Iran. e-mail: mehdi.valleh@uoz.ac.ir
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Summary

The effects of the paternal breed on early embryo and later pre- and postnatal development are well documented. Several recent studies have suggested that such paternal effects may be mediated by the paternally induced epigenetic modifications during early embryogenesis. The objective of this study was to investigate the effects of the paternal breed on the early embryonic development and relative expression of the maternally imprinted gene, IGF-II, and the apoptosis-related genes BAK1 and BCL2-L1 in in vitro produced (IVP) bovine embryos derived from two unrelated paternal breeds (Holstein and Brown Swiss). The degree of correlation of IGF-II expression pattern with embryo developmental competence and apoptosis-related genes was also investigated. The relative abundance of IGF-II, BCL2-L1 and BAK1 transcripts in day 8 embryos was measured by quantitative reverse-transcription polymerase chain reaction using the comparative Cp method. Our data revealed that the paternal breed did not influence cleavage rate, blastocyst rate and relative abundance of IGF-II, BAK1 and BCL2-L1 in day 8 blastocysts (P > 0.05). Nevertheless, IGF-II expression levels were highly correlated with embryonic developmental competence (r = 0.66, P < 0.1), relative expression of BCL2-L1 (r = 0.72, P < 0.05) and ratio of BCL2-L1/BAK1 (r = 0.78, P < 0.05). In conclusion, our data show that IGF-II, BCL2-L1 and BAK1 expression is not related to the chosen combination of paternal breed, but that IGF-II expression is correlated with embryonic viability and apoptosis-related gene expression.

Keywords

BAK1BCL2-L1Bovine blastocystIGF-IIPaternal breed
Type
Research Article
Information
Zygote , Volume 23 , Issue 5 , October 2015 , pp. 712 - 721
Copyright
Copyright © Cambridge University Press 2014 

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