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Persistence of human mitochondrial DNA throughout the development to the blastocyst of mouse zygotes microinjected with human mitochondria

Published online by Cambridge University Press:  01 November 1999

V.B. Vasilyev ,
V.A. Sokolova ,
A.V. Sorokin ,
M.G. Bass ,
N.I. Arbuzova ,
E.l. Patkin ,
V.I. Golubkov ,
A.P. Dyban  and
V.S. Gaitskhoki
V.B. Vasilyev
Affiliation:
Department of Molecular Genetics, Institute for Experimental Medicine, 12, Pavlov Street, Saint Petersburg, 197376 Russia.
V.A. Sokolova
Affiliation:
Department of Molecular Genetics, Institute for Experimental Medicine, 12, Pavlov Street, Saint Petersburg, 197376 Russia.
A.V. Sorokin
Affiliation:
Department of Molecular Genetics, Institute for Experimental Medicine, 12, Pavlov Street, Saint Petersburg, 197376 Russia.
M.G. Bass
Affiliation:
Department of Molecular Genetics, Institute for Experimental Medicine, 12, Pavlov Street, Saint Petersburg, 197376 Russia.
N.I. Arbuzova
Affiliation:
Department of Molecular Genetics, Institute for Experimental Medicine, 12, Pavlov Street, Saint Petersburg, 197376 Russia.
E.l. Patkin
Affiliation:
Department of Molecular Genetics, Institute for Experimental Medicine, 12, Pavlov Street, Saint Petersburg, 197376 Russia.
V.I. Golubkov
Affiliation:
Department of Molecular Genetics, Institute for Experimental Medicine, 12, Pavlov Street, Saint Petersburg, 197376 Russia.
A.P. Dyban
Affiliation:
Department of Molecular Genetics, Institute for Experimental Medicine, 12, Pavlov Street, Saint Petersburg, 197376 Russia.
V.S. Gaitskhoki
Affiliation:
Department of Molecular Genetics, Institute for Experimental Medicine, 12, Pavlov Street, Saint Petersburg, 197376 Russia.
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Abstract

The conditions for transfer of human mitochondria into fertilised mouse ova were elaborated. Species-specific primers were designed to discriminate human mitochondrial DNA (mtDNA) and the endogenous mtDNA in the preimplantation embryos. Human mitochondria isolated from the HepG2 cell line were microinjected into murine zygotes, and the latter cultured for 96 h to the blastocyst stage. The polymerase chain reaction allowed the detection of human mtDNA at every stage of embryo cleavage. In some cases a clear disparity in distribution of human mtDNA among blastomeres was evident.

Keywords

Human mtDNA transferMitochondria microinjectionPreimplantation developmentTransgenic mice
Type
Research Article
Information
Zygote , Volume 7 , Issue 4 , November 1999 , pp. 279 - 283
Copyright
1999 Cambridge University Press

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