Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-10T12:11:52.600Z Has data issue: false hasContentIssue false
  • English
  • Français

Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814)

Published online by Cambridge University Press:  02 December 2019

Thaís T.S. Souza ,
Maria J.B. Bezerra ,
Maurício F. van Tilburg ,
Celso S. Nagano ,
Luciana D. Rola ,
José M.B. Duarte ,
Luciana M. Melo ,
Arlindo A. Moura  and
Vicente J.F. Freitas Open the ORCID record for Vicente J.F. Freitas [Opens in a new window]
Thaís T.S. Souza
Affiliation:
Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil
Maria J.B. Bezerra
Affiliation:
Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil
Maurício F. van Tilburg
Affiliation:
Health Sciences Center, State University of Ceará, Fortaleza, Brazil
Celso S. Nagano
Affiliation:
Laboratory of Mass Spectrometry, Federal University of Ceará, Fortaleza, Brazil
Luciana D. Rola
Affiliation:
Department of Animal Science, State University of São Paulo, Jaboticabal, Brazil
José M.B. Duarte
Affiliation:
Department of Animal Science, State University of São Paulo, Jaboticabal, Brazil
Luciana M. Melo
Affiliation:
Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil Molecular Genetics Research Unit, University Center Fametro, Fortaleza, Brazil
Arlindo A. Moura
Affiliation:
Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil
Vicente J.F. Freitas*
Affiliation:
Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil
*
Author for correspondence: V.J.F. Freitas. Faculty of Veterinary, State University of Ceará, Av. Dr. Silas Munguba, 1700, 60714-903, Fortaleza, Ceará, Brazil. Tel: +55 85 31019861. E-mail: vicente.freitas@uece.br
Get access Rights & Permissions [Opens in a new window]

Summary

The aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (≤3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P > 0.05) was observed between small/medium and large follicles: 49.2 ± 22.8 and 56.7 ± 27.4 μg/μl, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P > 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer.

Keywords

DeerElectrophoresisMS/MSSDS-PAGE
Type
Short Communication
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 170 - 173
Copyright
© Cambridge University Press 2019

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

Present address: Federal University of Paraiba, Areia, Brazil.

References

Aquino-Cortez, A, Pinheiro, BQ, Lima, DBC, Silva, HVR, Mota-Filho, AC, Martins, JAM, Rodriguez-Villamil, P, Moura, AA and Silva, LDM (2017) Proteomic characterization of canine seminal plasma. Theriogenology 95, 178–86.CrossRefGoogle ScholarPubMed
Black-Décima, P, Rossi, RV, Vogliotti, A, Cartes, JL, Maffei, L, Duarte, JMB and Juliá, JP (2010) Brown brocket deer Mazama gouazoubira (Fischer 1814). In Duarte, JMD and González, S (eds) Neotropical Cervidology. Jaboticabal: FUNEP, pp 119–32.Google Scholar
Bradford, MM (1976) A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of dye binding. Anal Biochem 72, 248–54.CrossRefGoogle Scholar
Fahiminiya, S and Gérard, N (2010) Follicular fluid in mammals. Gynecol Obstet Fertil 38, 402–4.CrossRefGoogle ScholarPubMed
Fahiminiya, S, Reynaud, K, Labas, V, Batard, S, Chastant-Maillard, S and Gérard, N (2010) Steroid hormones content and proteomic analysis of canine follicular fluid during the preovulatory period. Reprod Biol Endocrinol 8, 132–46.CrossRefGoogle ScholarPubMed
Fahiminiya, S, Labas, V, Roche, S, Dacheux, JL and Gérard, N (2011) Proteomic analysis of mare follicular fluid during late follicle development. Proteome Sci 9, 54–73.CrossRefGoogle ScholarPubMed
Ferrazza, RA, Garcia, HDM, Schmidt, EMDS, Mihm Carmichael, M, Souza, FF, Burchmore, R, Sartori, R, Eckersall, PD and Ferreira, JCP (2017) Quantitative proteomic profiling of bovine follicular fluid during follicle development. Biol Reprod 97, 835–49.CrossRefGoogle ScholarPubMed
Fu, Q, Huang, Y, Wang, Z, Chen, F, Huang, D, Lu, Y, Liang, X and Zhang, M (2016) Proteome profile and quantitative proteomic analysis of buffalo (Bubalus bubalis) follicular fluid during follicle development. Int J Mol Sci 17, 618–38.CrossRefGoogle ScholarPubMed
Ginther, OJ, Kot, K, Kulick, LJ and Wiltbank, MC (1997) Sampling follicular fluid without altering follicular status in cattle: oestradiol concentrations early in a follicular wave. J Reprod Fert 109, 181–6.CrossRefGoogle Scholar
Handrieder, J, Nyakas, A, Naessén, T and Bergquist, J (2008) Proteomic analysis of human follicular fluid using an alternative bottom-up approach. J Proteome Res 7, 443–9.CrossRefGoogle Scholar
IUCN Red List of Threatened Species (2016) Available at http://wwwiucnredlistorg. Accessed 4 January 2019.Google Scholar
Levay, PF, Huyser, C, Fourie, FL and Rossouw, DJ (1997) The detection of blood contamination in human follicular fluid. J Assist Reprod Genet 14, 212–7.CrossRefGoogle ScholarPubMed
Mortarino, M, Vigo, D, Maffeo, G and Ronchi, S (1999) Two-dimensional polyacrylamide gel electrophoresis map of bovine ovarian fluid proteins. Electrophoresis 20, 866–9.3.0.CO;2-V>CrossRefGoogle ScholarPubMed
Murata, Y, Magner, JA and Refetoff, S (1986) The role of glycosylation in the molecular conformation and secretion of thyroxine-binding globulin. Endocrinology 118, 1614–21.CrossRefGoogle ScholarPubMed
Paula Junior, AR, Van Tilburg, MF, Lobo, MDP, Monteiro-Moreira, AC, Moreira, RA, Melo, CH, Souza-Fabjan, JM, Araújo, AA, Melo, LM, Teixeira, DIA, Moura, AA and Freitas, VJF (2018) Proteomic analysis of follicular fluid from tropically-adapted goats. Anim Reprod Sci 188, 35–44.CrossRefGoogle ScholarPubMed
Pereira, RJ, Polegato, BF, Souza, S, Negrão, JA and Duarte, JM (2006) Monitoring ovarian cycles and pregnancy in brown brocket deer (Mazama gouazoubira) by measurement of fecal progesterone metabolites. Theriogenology 65, 387–99.CrossRefGoogle ScholarPubMed
Sánchez, L, Calvo, M and Brock, JH (1992) Biological role of lactoferrin. Arch Dis Child 67, 657–61.CrossRefGoogle ScholarPubMed
SEMA Portaria SEMA nº 001 de 9 de junho de 1998; Rio de Janeiro: 1998 Lista das Espécies Ameaçadas de Extinção no Estado do Rio de Janeiro.Google Scholar
Sun, YL, Ping, ZG, Li, CJ, Sun, YF, Yi, KL, Chen, L, Li, XY, Wang, XL and Zhou, X (2011) Comparative proteomic analysis of follicular fluids from normal and cystic follicles in sows. Reprod Domest Anim 46, 889–95.CrossRefGoogle ScholarPubMed
Wu, Y, Lin, J, Han, B, Wang, L, Chen, Y, Liu, M and Huanget, J (2018) Proteomic profiling of follicle fluids after superstimulation in one-month-old lambs. Reprod Domest Anim 53, 186–94.CrossRefGoogle ScholarPubMed
Zanetti, ES, Polegato, BF and Duarte, JM (2010) Comparison of two methods of synchronization of estrus in brown brocket deer (Mazama gouazoubira). Anim Reprod Sci 117, 266–74.CrossRefGoogle Scholar
Zanetti, ES and Duarte, JM (2011) Comparison of three protocols for superovulation of brown brocket deer (Mazama gouazoubira). Zoo Biol 31, 642–55.CrossRefGoogle Scholar
Supplementary material: File

Souza et al. Supplementary material

Table S1

Download Souza et al. Supplementary material(File)
File 59.6 KB