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A new isolate of Mesorhabditis monhystera (Bütschli, 1873) Dougherty, 1955 (Rhabditida: Rhabditidae): re-evaluated with molecular data and scanning electron microscopic observations

Published online by Cambridge University Press:  26 January 2023

I. Bashir ,
M. Mahboob Open the ORCID record for M. Mahboob [Opens in a new window]  and
Q. Tahseen Open the ORCID record for Q. Tahseen [Opens in a new window]
I. Bashir
Affiliation:
Nematode Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India
M. Mahboob
Affiliation:
Nematode Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India
Q. Tahseen*
Affiliation:
Nematode Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India
*
Author for correspondence: Q. Tahseen, E-mail: qtahseen@gmail.com
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Abstract

A new isolate of Mesorhabditis monhystera (Bütschli, 1873) Dougherty, 1955 is described and illustrated with morphological and molecular data. The phylogenetic analysis based on the D2/D3 segment of 28S rDNA using the Bayesian inference method, revealed monophyly of the genus Mesorhabditis as the subordinate taxa clustered in one clade. The clade further divided into two subclades representing the Monhystera-group and Spiculigera-group with 100% posterior probability values. However, GenBank sequences of several species constituting the Monhystera-group, showed high similarity and very little genetic divergence (98–99%) of up to 4–5 bases. In order to ascertain the status of those isolates, detailed morphological comparison is provided along with a pictorial key. A sequence-based phylogeography of haplogroups of Mesorhabditis using the median-joining network method, was also inferred. The results suggested the need for morphological validation of a species before its sequences are deposited in GenBank.

Keywords

BiogeographyD2/D3 regionhaplotype networkmorphologymolecularphylogenyscanning electron microscopy
Type
Research Paper
Information
Journal of Helminthology , Volume 97 , 2023 , e11
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press

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