Associations of serum 25-hydroxycholecalciferol and parathyroid hormone with serum lipids differ by sex and vitamin D status

Abstract

Objective

Although emerging evidence indicates an association between vitamin D and serum lipids, the data are still inconsistent. The purpose of the present study was to investigate whether 25-hydroxycholecalciferol (25-hydroxyvitamin D₃; 25(OH)D₃) or intact parathyroid hormone (iPTH) was independently related to serum lipids in elderly women and men.

Design

Cross-sectional study. Fasting serum levels of 25(OH)D₃, iPTH, TAG, total cholesterol (TC), HDL cholesterol (HDL-C) and LDL cholesterol (LDL-C) were assessed. Body composition was measured by bioelectrical impedance analysis. Lifestyle factors, such as nutrient intake, time spent outdoors, physical activity, smoking, supplement intake and medication, were assessed by questionnaires. Multiple regression analyses were performed to examine associations of 25(OH)D₃ and iPTH with serum lipids.

Setting

Giessen, Germany.

Subjects

One hundred and ninety-three well-functioning German elderly aged 66–96 years.

Results

After adjusting for age, body fat, physical activity, smoking, alcohol intake, lipid-modifying drugs and either iPTH or 25(OH)D₃, 25(OH)D₃ was a predictor of TAG (standardised coefficient beta (β)=–0·180), HDL-C (β=0·197), LDL-C:HDL-C (β=−0·298) and TC:HDL-C (β=−0·302) in women, whereas iPTH was a predictor of HDL-C (β=−0·297) in men. In sub-analysis, associations between 25(OH)D₃ and TC (β=−0·252), HDL-C (β=0·230), LDL-C (β=−0·324), LDL-C:HDL-C (β=−0·412) and TC:HDL-C (β=−0·380) were found in women with 25(OH)D₃ concentrations above or equal the median vitamin D status (62·3 nmol/l), but not in women with lower 25(OH)D₃ concentrations.

Conclusions

In the elderly, associations of 25(OH)D₃ and iPTH with serum lipids may differ by sex and may require a vitamin D status above 62 nmol/l.

Accumulating epidemiological data indicate that vitamin D deficiency may be accompanied by susceptibility for chronic diseases^{( 1 )}. Recently, a low vitamin D status has been linked to increased serum levels of TAG, total cholesterol (TC) and LDL cholesterol (LDL-C), as well as low concentrations of HDL cholesterol (HDL-C)^{( 2 – 8 )}. Parathyroid hormone (PTH), which increases in the presence of vitamin D insufficiency, was also linked to serum lipids^{( 9 )}. However, the results are inconsistent and associations often vanished after controlling for confounding variables^{( 5 , 6 , 8 )}.

The question arises whether the assumed associations between serum lipids and vitamin D and PTH are independent from other cardiovascular risk factors like obesity, which has been associated with elevated serum lipids^{( 10 )} and may also interfere with the vitamin D endocrine system^{( 11 )}. In addition, the associations may differ by sex considering the differences in body composition and serum lipid levels between women and men. Previous studies were mostly performed on ambulatory patients and/or vitamin D-deficient, middle-aged or overweight and obese individuals and often without adjustments for potential confounders, such as the association between vitamin D and PTH, body composition, medical conditions and/or lifestyle factors, including sun exposure, physical activity and habitual diet^{( 4 – 7 , 9 , 12 – 15 )}.

As dyslipidaemia is an important CVD risk factor and an established component of the metabolic syndrome, treatment and prevention strategies are of particular importance^{( 16 )}. This applies especially to elderly people, who are at risk of developing dyslipidaemia. Moreover, an advanced age is linked to vitamin D deficiency because of age-related declines in endogenous vitamin D synthesis, sun exposure and dietary intake^{( 17 )}, which in turn may lead to a rise in PTH. However, studies in which the associations of vitamin D and PTH with serum lipids and lipid ratios are simultaneously examined in well-functioning elderly persons are scarce.

The objective of our study was to analyse whether vitamin D status, as measured by serum 25-hydroxycholecalciferol (25-hydroxyvitamin D₃; 25(OH)D₃) levels, and the levels of intact PTH (iPTH) are related to serum lipids and lipid ratios in non-institutionalised elderly people. Thereby, we took into account that these associations may depend on sex, age, body composition or lifestyle. Furthermore, as some experts hypothesised that the associations between vitamin D and serum lipids may be more pronounced in vitamin D-deficient individuals^{( 18 )}, we tested the hypothesis that associations may only occur at lower 25(OH)D₃ levels.

Methods

Study population and design

Participants were drawn from an ongoing cohort study in which the nutrition and health status of senior citizens in Giessen (GISELA), Germany (latitude 50·6°N) have been observed at annual intervals since 1994 and at biannual intervals since 1998. The GISELA study is a non-intervening observational study. The investigations took place at the Institute of Nutritional Science in Giessen, Germany. Participants were recruited by physicians, notices, senior citizens’ meetings, advertisements in local newspapers and through individuals who had already participated. Recruitment was continued until 2004 with a total number of 584 participants enrolled. Not all individuals participated in each follow-up. For enrolment, individuals had to be at least 60 years of age and physically mobile. The study was conducted according to the guidelines laid down in the Declaration of Helsinki and approved by the Ethical Committee of the Faculty of Medicine at the Justus-Liebig-University, Giessen, Germany. All participants provided written informed consent.

The present investigation reports cross-sectional data that were obtained in the summer of 2008 from July to October. Participants with missing data (n 73) on blood samples, food record, body composition, chronic kidney disease history and lifestyle factors, including time spent outdoors, physical activity and smoking history, were excluded, as were those who suffered from chronic kidney disease (n 5). In addition, two elderly people were excluded because their TAG concentrations were >400 mg/dl and two elderly people were identified as outliers due to their deviation from the mean values of the cohort and/or from the normal distribution of the residuals of the regression analyses (one person with an iPTH value of 0·6 pmol/l and one with a LDL-C:HDL-C of 5·4 as well as a TC:HDL-C of 8·2) and were therefore not included. Of the 275 elderly people who took part in the follow-up in 2008, data from 138 women and fifty-five men were included in the following analysis.

Anthropometric data and body composition

BMI was calculated as the measured weight in kilograms divided by the square of the measured body height in metres^{( 11 )}. Waist circumference (WC) was determined in an upright position by a tape measure to the nearest 1·0 cm. The percentage of total body fat (%TBF) was recorded by a single-frequency (50 kHz) bioelectrical impedance analyser (Akern-RJL BIA 101/S; Data Input, Frankfurt, Germany) according to the manufacturer’s instructions and the predictive formula from Roubenoff et al. ^{( 19 )}.

Laboratory measurements

Blood samples were collected after an overnight fast and immediately centrifuged, aliquoted and frozen at −70°C until further analysis. Serum concentrations of both 25(OH)D₃ and iPTH were measured by an electrochemiluminescence immunoassay (Roche Diagnostics GmbH, Mannheim, Germany) in the Limbach Laboratory, Heidelberg, Germany, which participates in the Vitamin D External Quality Assessment Scheme (DEQAS). This assay has been standardised against LC–MS/MS^{( 20 )}. The CV for the total analytic precision of this assay was ≤9·9 % for 25(OH)D₃ and ≤5·9 % for iPTH^{( 20 , 21 )}. The lower detection limit of this assay was 10·0 nmol/l for 25(OH)D₃ and 0·127 pmol/l for iPTH. Concentrations of 25(OH)D₃ <25 nmol/l were defined as vitamin D deficiency^{( 22 )}. Due to the lack of an international consensus on optimal 25(OH)D₃ status, we applied two 25(OH)D₃ cut-off values as adequate (≥50 nmol/l^{( 23 )} and ≥75 nmol/l^{( 24 )}). The fasting serum concentrations of TAG, TC and HDL-C (after precipitation with phosphotungstic acid and Mg²⁺ ions) were measured using commercial enzymatic methods, such as GPO-PAP for TAG and CHOD-PAP for TC and HDL-C, according to the manufacturer’s procedures (Roche Diagnostics GmbH). The cut-off values for elevated TAG and TC levels were set at ≥200 mg/dl and ≥240 mg/dl, respectively, whereas HDL-C levels <50 mg/dl and <40 mg/dl were classified as low for women and men, respectively^{( 25 – 27 )}. The levels of LDL-C were indirectly ascertained by the Friedewald equation^{( 28 )} as follows: LDL-C = TC−HDL-C−TAG/5; all participants had TAG levels <400 mg/dl. LDL-C concentrations ≥160 mg/dl were considered as elevated^{( 27 )}. In addition, ratios of LDL-C to HDL-C and TC to HDL-C were determined.

Lifestyle factors

Intakes of vitamin D, SFA and alcohol were determined by a 3 d estimated dietary record, which was developed and validated for the GISELA study^{( 29 )}. This record did not assess nutrient intake from supplements. Using self-administered questionnaires data on age, time spent outdoors, smoking behaviour, physical activity patterns, diseases, medications and vitamin D supplement intake were collected. We assessed the daily time spent outdoors as a proxy for the daily sun exposure by asking the following question: ‘Please estimate, how much time do you currently spend outdoors in minutes per day?’ The smoking behaviour was classified as a dichotomous variable in constant non-smoker v. current and ex-smoker. The use of lipid-modifying drugs and the intake of vitamin D supplements were also coded as dichotomous variables (no/yes). The physical activity level (PAL) of each participant was assessed as described elsewhere^{( 30 )}.

Statistical analysis

The characteristics of the study population were expressed as medians and 5th–95th percentiles due to non-normally distributed data, which was tested by the Kolmogorov–Smirnov test. Descriptive characteristics were compared between groups via the Mann–Whitney U test for continuous variables and via the χ ² test or Fisher’s exact test for categorical variables.

At first, we examined univariate associations of either 25(OH)D₃ or iPTH as the independent variable with the respective serum lipid as the dependent variable. Subsequently, stepwise multiple regression analyses were performed with serum concentrations of 25(OH)D₃ and iPTH simultaneously included as fixed independent variables in each of the models and the respective serum lipid as the dependent variable. Sex, age, %TBF, PAL, time spent outdoors, intake of SFA, vitamin D and alcohol, smoking behaviour and the use of lipid-modifying drugs were considered as covariates and were allowed to remain in the model with a P value ≤0·100. In consideration of the observed collinearity of BMI, WC and %TBF (data not shown), the variable that showed the strongest association with serum lipids was added as a potential confounder to the regression model. Effect modification by sex was evaluated by stratified analyses and tests for statistical interaction by adding product terms (sex×25(OH)D₃ and sex×iPTH) into the multiple regression analyses, respectively. Because of skewed distributions, TAG and HDL-C were logarithmically transformed (log₁₀).

Finally, we examined whether the associations between 25(OH)D₃ and serum lipids differed between participants with 25(OH)D₃ levels below and above the sex-specific median 25(OH)D₃ status by using stepwise multiple regression analyses with adjustments for age, %TBF, PAL, alcohol consumption, smoking behaviour, use of lipid-modifying drugs and iPTH. The median 25(OH)D₃ status was used as cut-off value to get two groups almost equal in size.

Statistical analyses were conducted using the statistical software package IBM SPSS^® Statistics version 21·0 for Windows. The significance level was set at P<0·05, and two-sided.

Results

Characteristics of the study participants

The characteristics of the study participants are shown in Table 1. None of the participants were vitamin D-deficient, but 20·7 % had 25(OH)D₃ levels <50 nmol/l and 20·7 % had 25(OH)D₃ levels ≥75 nmol/l. Hyperparathyroidism, which was defined as iPTH levels >6·9 pmol/l, was found in 15·5 % of the participants. Of the study population, 6·2 %, 13·5 % and 7·3 % had elevated levels of TAG, TC and LDL-C, respectively, and 24·9 % showed low HDL-C levels. No significant sex differences were observed in any of these analyses (data not shown).

Table 1 Characteristics of the study population*: well-functioning German elderly aged 66–96 years (n 193), Giessen, Germany, July–October 2008 (GISELA study)

WC, waist circumference; %TBF, percentage of total body fat; 25(OH)D₃, 25-hydroxycholecalciferol (25-hydroxyvitamin D₃); iPTH, intact parathyroid hormone; TC, total cholesterol; HDL-C, HDL cholesterol; LDL-C, LDL cholesterol; PAL, physical activity level.

* Data are presented as median and 5th–95th percentiles (P5–P95) for continuous variables and as absolute and relative frequencies for categorical variables.

† Mann–Whitney U test for continuous variables, χ ² test for categorical variables.

‡ The term dyslipidaemia refers to participants with serum levels of TAG≥200 mg/dl, TC≥240 mg/dl, LDL-C ≥ 160 mg/dl and/or HDL-C<40 (men) and 50 (women) mg/dl, respectively.

No differences in serum lipid concentrations were found between participants who had 25(OH)D₃ levels of ≥50 nmol/l and <50 nmol/l. In contrast, the ratios of LDL-C:HDL-C (median: 1·96 v. 2·21; P=0·022) and TC:HDL-C (median: 3·28 v. 3·68; P=0·031) were significantly lower in participants with 25(OH)D₃ concentrations ≥75 nmol/l compared with those who had lower 25(OH)D₃ levels.

Participants with dyslipidaemia (low HDL-C levels, elevated levels of TAG, TC and/or LDL-C) or participants who used lipid-modifying drugs did not differ in their prevalence of vitamin D insufficiency (25(OH)D₃ levels <50 or <75 nmol/l) from those who showed normal lipid profiles or did not use lipid-modifying drugs. However, participants with dyslipidaemia had lower 25(OH)D₃ levels than those with lipid concentrations in the reference range (median: 59·9 v. 65·5 nmol/l; P=0·017). The use of lipid-modifying drugs had no impact on 25(OH)D₃ in the sex- and age-adjusted regression analysis (data not shown).

Univariate and multiple regression analyses of 25-hydroxycholecalciferol and intact parathyroid hormone with serum lipids and lipid ratios for the entire study population and stratified by sex

The results of the univariate regression analyses regarding the associations of 25(OH)D₃ and iPTH with serum lipids are given in Table 2. Serum 25(OH)D₃ was positively associated with log HDL-C and negatively associated with ratios of LDL-C:HDL-C and TC:HDL-C in women and in the pooled analysis of women and men together. In addition, serum 25(OH)D₃ was negatively associated with log TAG in women. Serum iPTH was negatively associated with log HDL-C in men and in the pooled analysis.

Table 2 Univariate regression analyses of 25(OH)D₃ and iPTH with fasting serum lipids* among well-functioning German elderly aged 66–96 years (n 193), Giessen, Germany, July–October 2008 (GISELA study)

25(OH)D₃, 25-hydroxycholecalciferol (25-hydroxyvitamin D₃); iPTH, intact parathyroid hormone; TC, total cholesterol; HDL-C, HDL cholesterol; LDL-C, LDL cholesterol.

* The results of the analyses are expressed in terms of the standardised coefficient beta (β).

As %TBF was more strongly associated with serum lipids than were anthropometric variables as judged by the standardised coefficient beta (β; data not shown), and because %TBF, but not anthropometric parameters, was an independent determinant of 25(OH)D₃ in this cohort^{( 11 )}, the subsequent multiple regression analyses were controlled for %TBF.

Table 3 presents the results of the multiple regression analyses for the entire study population and, in addition, stratified by sex. Substantial sex differences existed and effect modification by sex was significant with respect to log HDL-C, LDL-C and LDL-C:HDL-C (P<0·05). In women, 25(OH)D₃ was a predictor of log TAG, log HDL-C, LDL-C:HDL-C and TC:HDL-C, whereas, in men, iPTH was a negative predictor of log HDL-C after adjusting for age, %TBF, PAL, alcohol consumption, smoking behaviour and the use of lipid-modifying drugs. Except for the association between iPTH and TC in men, which became significant (β=−0·281; P=0·035), these results remained essentially unchanged after additional adjustment for time spent outdoors, intake of SFA, dietary vitamin D intake and use of vitamin D supplements (data not shown). Because 25(OH)D₃ was associated with serum lipids only in women, the following sub-analysis was restricted to female participants.

Table 3 Multiple regression analyses to scrutinise independent associations of 25(OH)D₃ and iPTH with fasting serum lipids* among well-functioning German elderly aged 66–96 years (n 193), Giessen, Germany, July–October 2008 (GISELA study)

25(OH)D₃, 25-hydroxycholecalciferol (25-hydroxyvitamin D₃); iPTH, intact parathyroid hormone; TC, total cholesterol; HDL-C, HDL cholesterol; LDL-C, LDL cholesterol; %TBF, percentage of total body fat; PAL, physical activity level.

* Multiple linear regression analyses were performed using stepwise procedure and the respective serum lipid as dependent variable. Serum 25(OH)D₃ and iPTH concentrations were simultaneously included as fixed independent variables in each of the models. The results of the analyses are expressed in terms of the standardised coefficient beta (β) and the adjusted coefficient of determination (R ²) for the final model provided that the association of 25(OH)D₃ or iPTH with serum lipids reached statistical significance.

† Adjusted for sex, sex interaction (sex×25(OH)D₃ and sex×iPTH), age, %TBF, PAL, alcohol consumption, smoking behaviour and the use of lipid-modifying drugs. Effect modification by sex was significant for log HDL-C (sex×25(OH)D₃; P<0·0001), LDL-C (sex×iPTH; P=0·017) and LDL-C:HDL-C (sex×25(OH)D₃; P=0·006).

‡ Adjusted for age, %TBF, PAL, alcohol consumption, smoking behaviour and the use of lipid-modifying drugs.

Associations between 25-hydroxycholecalciferol and serum lipids stratified by median vitamin D status in women

In women, 25(OH)D₃ was significantly related to TC (β=−0·252; P=0·030), log HDL-C (β=0·230; P=0·046), LDL-C (β=−0·324; P=0·006), LDL-C:HDL-C (β=−0·412; P<0·001) and TC:HDL-C (β=−0·380; P=0·001) in participants with 25(OH)D₃ levels ≥62·3 nmol/l (n 69) after controlling for age, %TBF, PAL, alcohol consumption, smoking behaviour, use of lipid-modifying drugs and iPTH. Female participants with 25(OH)D₃ levels <62·3 nmol/l (n 69) showed no significant associations. The results of the respective univariate regression analyses are illustrated in the online supplementary material, Supplemental Fig. 1.

Discussion

To our knowledge, the present study is the first one to investigate the associations of 25(OH)D₃ and iPTH with serum lipids and lipid ratios by sex and vitamin D status in primarily non-obese, non-vitamin D-deficient, well-functioning German elderly. Our results indicate that: (i) 25(OH)D₃ is associated with serum lipids only in women, whereas iPTH is associated with serum lipids only in men; and (ii) associations between 25(OH)D₃ and serum lipids may require 25(OH)D₃ concentrations above 62 nmol/l to significantly affect the serum lipid profile in women. Thus, contrary to the initial assumption, significant associations between 25(OH)D₃ and serum lipids may not be restricted to a low/insufficient vitamin D status. However, we cannot exclude that the true threshold level of 25(OH)D₃ is below or above 62 nmol/l or that vitamin D-deficient individuals show associations as well, because none of our participants had a severe vitamin D deficiency. Supplemental Fig. 1 (see online supplementary material) illustrates that non-linear relationships may exist, which could explain the conflicting results in the literature. In this context, one might speculate whether there is an association in deficient and sufficient stages and no significant association at concentrations between 25 and about 60 nmol/l.

The reason why 25(OH)D₃ was positively associated with HDL-C and negatively associated with TAG, LDL-C:HDL-C and TC:HDL-C only in women, whereas iPTH showed a negative relationship with TC and HDL-C only in men, is unknown. Possibly, the sex differences in HDL-C levels and in the amount and distribution of body fat act as confounders. Next to sex, %TBF was one of the strongest predictors of serum lipids in our cohort (data not shown), and an inverse association between %TBF and 25(OH)D₃ was observed only in the female participants of the GISELA study^{( 11 )}. Obesity is an established risk factor of dyslipidaemia^{( 10 )} and may negatively impact circulating 25(OH)D₃ levels^{( 11 )} due to a sequestration of vitamin D in adipose tissue or, alternatively, a volumetric dilution effect^{( 31 )}. Thus, a confounding effect of TBF seems likely. However, the greater sample size of women compared with men may have led to the observed sex differences.

Previous studies have reported conflicting findings regarding the associations between vitamin D, iPTH and serum lipids. For instance, in the Framingham Offspring Study, age- and sex-adjusted associations between 25-hydroxyvitamin D (25(OH)D) and TAG and HDL-C were reported in 678 individuals (mean age of 59·6 (sd 0·3) years) not using cholesterol-lowering medications, but these associations vanished after additional adjustments for BMI, WC and smoking^{( 8 )}. In the National Health and Nutrition Examination Survey, an independent association between 25(OH)D and HDL-C was reported^{( 4 , 5 )}, but no associations between 25(OH)D and TAG or LDL-C^{( 5 )}. In the Women’s Health Initiative Calcium–Vitamin D trial, a significant inverse association between 25(OH)D and TAG was found in 292 postmenopausal women aged 50–79 years after considering potential confounders, but no associations between 25(OH)D and TC, LDL-C or HDL-C^{( 32 )}. In the study by Karhapää et al. with 909 Finnish men aged 45–70 years, significant inverse associations were observed between 25(OH)D and TAG, TC and LDL-C, but no association between 25(OH)D and HDL-C was noticed after controlling for confounding factors^{( 7 )}. In the Tromsø Study, non-fasting serum levels of TC, HDL-C and LDL-C increased, while LDL-C:HDL-C and TAG levels decreased, across 25(OH)D quartiles in 8018 non-smokers with a mean age of 55·9 (sd 12·6) years after controlling for sex, age, sample month and BMI^{( 33 )}. In previous investigations, the associations between PTH and serum lipids were less often examined and frequently no independent relationships were found^{( 5 , 12 )}. In our male participants, iPTH remained a negative predictor of HDL-C even after controlling for relevant confounders. The consideration of different confounders and the fact that some studies examined only one sex and others analysed women and men together without considering potential effect modifications by sex may be partly responsible for the inconsistent results. Furthermore, as indicated by our results, the vitamin D status of an individual might also be an important factor in this context.

The associations between 25(OH)D concentrations and serum lipids point towards a potentially favourable effect of vitamin D supplements on CVD risk markers. However, the majority of previous intervention trials did not report a significant increase in HDL-C or a decrease in unfavourable serum lipids, such as TAG, TC or LDL-C, due to vitamin D supplementation^{( 34 – 36 )}. A recent meta-analysis of twelve randomised controlled trials evaluated the effects of vitamin D supplements on blood lipids and indicated that vitamin D supplementation could increase LDL-C levels, but may not have statistically significant effects on TAG, TC or HDL-C^{( 37 )}. In our study, neither the use of vitamin D supplements nor the dietary intake levels of vitamin D were significantly associated with serum lipids after adjusting for sex, age, %TBF, iPTH, PAL, time spent outdoors, intake of SFA and alcohol, smoking behaviour and the use of lipid-modifying drugs (data not shown). Considering the low vitamin D intake and the low percentage of supplement users in our study, these amounts may have been insufficient to affect 25(OH)D₃ levels^{( 11 )} and, consequently, to influence concentrations of serum lipids. Moreover, in a previous study, we could show that the use of vitamin D supplements was associated with 25(OH)D₃ levels in individuals with a BMI<25 kg/m^{2 ( 38 )}, but not in those with a higher BMI. The higher TBF may decrease the bioavailability of vitamin D and thus may constrain favourable effects of vitamin D supplements on serum lipids. Therefore, a beneficial effect of vitamin D supplements on serum lipids cannot be excluded and may depend on dosage, vitamin D status and/or body composition.

As serum lipids represent markers for cardiovascular events and participants with dyslipidaemia had lower levels of 25(OH)D₃ than participants with lipid concentrations in the reference range in our study, this might support the hypothesis of a protective role of 25(OH)D₃ regarding CVD risk. At present, the mechanisms for the potential associations between vitamin D, iPTH and serum lipids still await elucidation. The vitamin D status of an individual may represent a surrogate marker of cardiovascular health^{( 13 )}, but may not occupy a central position in the pathogenesis of dyslipidaemia. Some researchers considered circulating Ca as a causal factor for the association between vitamin D and cardiovascular risk factors^{( 15 )}; whereas others stated that this association may not be mediated by circulating Ca^{( 5 )}. The increased absorption of intestinal Ca due to a higher vitamin D status may reduce circulating TAG by decreasing hepatic TAG formation and secretion^{( 15 )}. Alternatively, the suppression of iPTH by a high vitamin D status may result in a reduction of serum TAG by increased peripheral elimination^{( 15 , 32 )}. Other tentative explanations are the inverse association of vitamin D with insulin resistance and the vitamin D-mediated increase in the expression of VLDL receptors^{( 3 , 7 , 39 )}.

The strengths of the present study include the evaluation of different serum lipids and also lipid ratios in independently living elderly without vitamin D deficiency, the conduction of sex-specific analyses and the consideration of a variety of possible confounders as well as the application of different 25(OH)D₃ cut-off levels. However, as with any cross-sectional study, we cannot infer causality. Our study subjects were participants of a prospective cohort study and thus not recruited by representative criteria. In general, prospective cohort studies are susceptible to attrition of participants and selection bias, especially in older cohorts. Due to the presence of missing data the sample size was relatively small and selection bias cannot be excluded as the participants were volunteers, had higher levels of education and physical activity, and were more often normal-weight and non-smokers than their peers in the general German population^{( 30 )}. We did not analyse lipoprotein particle composition, carry out apolipoprotein testing or perform adjustment for multiple testing due to the exploratory approach of our study. Further limitations are the use of self-reported data, the indirect ascertainment of sun exposure and physical activity, and missing information on the brand, exact dosage and duration of the use of vitamin D supplements and lipid-modifying drugs. Frequently, the use of immunoassays rather than LC–MS/MS for measuring 25(OH)D is a matter of dispute. Concerns have been raised regarding for example the interaction with other compounds, such as vitamin D-binding protein or the C3-epimer of 25(OH)D₃. In addition, variations in the matrix in which the measurement is performed can influence antibody binding and may produce a random error^{( 40 )}. However, our assay was standardised against LC–MS/MS^{( 20 )} and the laboratory participates in the DEQAS.

Conclusions

In conclusion, the present study indicates that the associations of 25(OH)D₃ and iPTH with serum lipids may differ by sex and vitamin D status. The associations between 25(OH)D₃ and lipids were predominantly observed in women, while the associations between iPTH and lipids were restricted to men. Only at 25(OH)D₃ levels ≥62 nmol/l were associations of 25(OH)D₃ with TC, HDL-C, LDL-C, LDL-C:HDL-C and TC:HDL-C observed independent of body composition and lifestyle in women. Therefore, sex and vitamin D status should be taken into account when analysing potentially beneficial effects of vitamin D on CVD risk markers. In clinical practice, especially overweight and obese elderly individuals who are at risk of developing dyslipidaemia, hyperparathyroidism and vitamin D deficiency might benefit from a routine vitamin D screening and, if necessary, vitamin D supplementation, to counteract low 25(OH)D₃ levels. Overall, an adequate vitamin D status may have an advantageous impact on dyslipidaemia in the elderly.