We consider the Vlasov equation in any spatial dimension, which has long been known [ZI76, Mor80, Gib81, MW82] to be an infinite-dimensional Hamiltonian system whose bracket structure is of Lie–Poisson type. In parallel, it is classical that the Vlasov equation is a mean-field limit for a pairwise interacting Newtonian system. Motivated by this knowledge, we provide a rigorous derivation of the Hamiltonian structure of the Vlasov equation, both the Hamiltonian functional and Poisson bracket, directly from the many-body problem. One may view this work as a classical counterpart to [MNP+20], which provided a rigorous derivation of the Hamiltonian structure of the cubic nonlinear Schrödinger equation from the many-body problem for interacting bosons in a certain infinite particle number limit, the first result of its kind. In particular, our work settles a question of Marsden, Morrison and Weinstein [MMW84] on providing a ‘statistical basis’ for the bracket structure of the Vlasov equation.