Brine shrimps (Artemia spp.) are aquatic crustaceans known as important intermediate hosts for a wide range of helminth species. From 2011 to 2021, 4,347 individuals of brine shrimp were collected for this study, investigating the diversity and infection rates of helminth species in Artemia spp. from hypersaline waters in southern and eastern Ukraine. Seven helminth species were found: six cestodes (Anomotaenia tringae, Eurycestus avoceti, Branchiopodataenia gvozdevi, Confluaria podicipina, Fimbriarioides tadornae, Hymenolepis s.l. stellorae) and one unidentified acuariid nematode (Acuariidae gen. sp.). All these helminths were recorded for the first time in intermediate hosts in Ukraine, although they had been known from other regions. Additionally, partial sequences of the 18S rDNA gene as well as the mitochondrial cytochrome c oxidase subunit 1 (cox1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) genes were obtained for varying numbers of cestode and nematode isolates for the first time. The overall prevalence of helminth infection in Artemia spp. was 21.9%, and the intensity ranged from one to three specimens.