Growth chamber experiments were conducted to assess the effects of foliage-only, soil-only, and foliage-plus-soil placements of amicarbazone on annual bluegrass and creeping bentgrass growth. Evaluated herbicide treatments included amicarbazone at 49 or 147 g ai ha−1, as well as bispyribac-sodium at 74 g ai ha−1 for comparative purposes. Data from this research agree with previous reports of amicarbazone plant uptake. Amicarbazone is absorbed via above- and belowground pathways; however, plant growth is inhibited more by root uptake. Compared to foliage-only amicarbazone placement, soil-only placement more than doubled reductions in aboveground biomass and root mass 56 d after treatment (DAT), whereas no differences were detected between placements including soil contact. Across all evaluated parameters in this research, amicarbazone (49 g ha−1) impacted creeping bentgrass growth similarly to bispyribac-sodium, whereas annual bluegrass growth was inhibited more by amicarbazone, suggesting it provides a more efficacious chemical option for end-user applications.