Introduction. This study was carried out in French Guiana, over ten years (1994–2003) by three institutions (SPV, FDGPC and CIRAD); it updates the current state of knowledge of Tephritidae (both Dacini and Toxotrypanini tribes) species present in this country. Materials and methods. The work was mainly conducted in inhabited areas (from the Brazilian border to the Surinamese border) where cultivated fruit crops are located. Specimens were obtained by adult trapping and fruit sampling in nearby orchards and at the edge of the rainforest. Trapping was done consistently for 10 years, while fruit sampling was a discontinuous activity. We present only the results for fruit sampling from three consecutive years (2001–2003) in which a total of 880 kg from 45 fruit species in 22 plant families were collected. Results. Twenty-nine plant species from fourteen plant families were found to be hosts of twenty-one Anastrepha species and one Bactrocera species, Bactrocera carambolae Drew and Hancock. During this period, no specimen of Ceratitis capitata (Wiedemann) was collected in traps or fruit samples. We registered the main fruit trees which were hosts for B. carambolae and Anastrepha spp. Five hymenopterous parasitoid species were identified. Among them, Diachasmimorpha longicaudata (Ashmead) (Hymenoptera, Braconidae) is an exotic species and was introduced into French Guiana in collaboration with Brazilian authorities (EMBRAPA) in 2000 and 2001 within the framework of a classical biological control program. Conclusion. Our data provide baseline information about the tephritid species of economic importance present in French Guiana and assist in developing potential future control programs of both the B. carambolae and Anastrepha species in the Amazon Basin. These preliminary results are discussed in the light of their implication for rainforest conservation efforts and also evolutionary relationships between fruit flies and their hosts.

Fopius arisanus is a solitary egg–pupal endoparasitoid that attacks several species of tephritid fruit flies, particularly Bactrocera spp. This species, indigenous from the Indo-Australian region, was introduced into Mexico for biological control purposes. From the standpoint of the ‘new associations’ concept this parasitoid has been evaluated against fruit flies in the Anastrepha complex. We investigated the specificity of F. arisanus responses to fruits infested with two species of Anastrepha. We examined whether fruit volatiles attractive to this parasitoid are induced as a result of fruit fly oviposition. We also investigated whether F. arisanus females are able to discriminate between the oviposition-induced volatiles from host eggs parasitised by conspecifics and volatiles from unparasitised eggs. All experiments were performed in a wind tunnel. Results showed that mango fruits infested with A. ludens eggs (2–3 days after egg deposition) were significantly more attractive to naïve F. arisanus females compared with non-infested fruits or fruits infested with larvae. In addition, guava fruits harbouring A. striata eggs were significantly more attractive to the parasitoid than non-infested fruits or fruits infested with larvae. Thus, the parasitoid was attracted to fruits with eggs, but fruit and fly species did not influence the parasitoid attraction. We also found that F. arisanus females were more attracted to fruits exposed to fertile A. ludens females (i.e. fruits with eggs inside) compared with fruits exposed to sterile females (i.e. fruits with no eggs inside) or fruits with mechanical damage. Parasitoid females were not attracted to A. ludens eggs. The results suggest that the presence of eggs induces volatiles that attract parasitoids. Finally, we found that F. arisanus was able to discriminate between fruits with unparasitised eggs vs. eggs parasitised by conspecifics, indicating that host discrimination could be mediated by olfactory cues.

The results presented in this paper refer to a host survey, lasting approximately three and a half years (February 2003–July 2006), undertaken in the Vale do Rio Doce Natural Reserve, a remnant area of the highly endangered Atlantic Rain Forest located in Linhares County, State of Espírito Santo, Brazil. A total of 330 fruit samples were collected from native plants, representing 248 species and 51 plant families. Myrtaceae was the most diverse family with 54 sampled species. Twenty-eight plant species, from ten families, are hosts of ten Anastrepha species and of Ceratitis capitata (Wiedemann). Among 33 associations between host plants and fruit flies, 20 constitute new records, including the records of host plants for A. fumipennis Lima and A. nascimentoi Zucchi. The findings were discussed in the light of their implications for rain forest conservation efforts and the study of evolutionary relationships between fruit flies and their hosts.

We studied the behaviour of three Anastrepha spp. (Diptera: Tephritidae) that were attracted to protein hydrolysate in glass McPhail traps, hung on an 8 m tall wild mango tree, Mangifera indica at three different heights. Out of 665 flies that landed on the exterior of the trap only 31 % were caught. Females represented 60.2 % of all flies trapped. The intermediate trap hung at 5m caught the most flies (57.5%). We observed a great deal of feeding activity (on trap, leaves, branches and fruit) including régurgitation and reingestion of liquid food droplets. When not on the trap surface, flies were more commonly seen on leaves than on branches and fruits. Inter- and intraspecific encounters between all Anastrepha spp. and both sexes were commonly recorded. Males were observed calling alone or in leks. All leks were formed on leaves and on two occasions included individuals of two species. Our observations suggested that the McPhail traps attracted different Anastrepha spp. with varying degrees of intensity.